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Hyperdynamic plasticity of chromatin proteins in pluripotent embryonic stem cells (2006)

Meshorer,
E.,
Yellajoshula,
D.,
George,
E.,
Scambler,
P.J.,
Brown,
D.T.,
Misteli,
T.

Abstract

Differentiation of embryonic stem (ES) cells from a pluripotent to a committed state involves global changes in genome expression patterns. Gene activity is critically determined by chromatin structure and interactions of chromatin binding proteins. Here, we show that major architectural chromatin proteins are hyperdynamic and bind loosely to chromatin in ES cells. Upon differentiation, the hyperdynamic proteins become immobilized on chromatin. Hyperdynamic binding is a property of pluripotent cells, but not of undifferentiated cells that are already lineage committed. ES cells lacking the nucleosome assembly factor HirA exhibit elevated levels of unbound histones, and formation of embryoid bodies is accelerated. In contrast, ES cells, in which the dynamic exchange of H1 is restricted, display differentiation arrest. We suggest that hyperdynamic binding of structural chromatin proteins is a functionally important hallmark of pluripotent ES cells that contributes to the maintenance of plasticity in undifferentiated ES cells and to establishing higher-order chromatin structure

Publication details

Download	http://www.ucl.ac.uk/research/publications/?action=search&pubid=114057
Repository	UCL Research Publications Index (MyOPIA) (United Kingdom)
Keywords	Animals, Blotting,Western, Cell Count, Cell Cycle, Cell Cycle Proteins, Cell Differentiation, Cells,Cultured, Chromatin, Chromatin Assembly and Disassembly, Chromosomal Proteins,Non-Histone, Comparative Study, deficiency, diagnostic use, Electrophoresis,Polyacrylamide Gel, Embryo, Embryonic Development, Gene Expression Regulation,Developmental, Green Fluorescent Proteins, Histone-Lysine N-Methyltransferase, Histones, Immunohistochemistry, In Situ Hybridization, Indoles, Interleukin-6, Intermediate Filament Proteins, Kinetics, metabolism, methods, Mice, Mice,Knockout, Molecular Biology, Nerve Tissue Proteins, Nonlinear Dynamics, Octamer Transcription Factor-3, physiology, Pluripotent Stem Cells, Protein Array Analysis, Research Support,N.I.H.,Intramural, Research Support,Non-U.S.Gov't, Research Support,U.S.Gov't,Non-P.H.S., Time Factors, Transcription Factors
Type	JOUR
Relation	105-116, 1, 10