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The kinetics and feedback inhibition of cytidine 5′-triphosphate synthetase in wild-type and mutant Chinese hamster cells (1984)

Abstract

The kinetics and cytidine 5′-triphosphate (CTP) feedback inhibition of CTP synthetase in wild-type and four mutants of Chinese hamster V79 cells have been studied. The enzymes of the wild type and three of the four mutants exhibited positive cooperativity with the substrate uridine 5′-triphosphate (UTP). Three of the mutants had K m app and S 50 valuves distinctly greater than those of the wild type, while the fourth mutant had values similar to those of the wild type. all four mutants exhibited resistance to CTP feedback inhibition, while the wild type was sensitive to such inhibition. It is postulated that a single mutational event in each mutant had caused a concomitant change of the enzyme in its binding both to the substrate UTP and to the end-product CTP.. Peer Reviewed. http://deepblue.lib.umich.edu/bitstream/2027.42/44151/1/10528_2004_Article_BF00485855.pdf

Publication details

Download	, http://hdl.handle.net/2027.42/44151 http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6497833&dopt=citation
Publisher	Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media
Contributors	Lawrence D. Buhl Center for Human Genetics, Department of Human Genetics, University of Michigan Medical School, 48109, Ann Arbor, Michigan, Lawrence D. Buhl Center for Human Genetics, Department of Human Genetics, University of Michigan Medical School, 48109, Ann Arbor, Michigan, Ann Arbor
Repository	University of Michigan (United States)
Keywords	CTP synthetase, Biochemistry, general, Biomedicine, Human Genetics, Medical Microbiology, Zoology, cytidine 5′-triphosphate (CTP), uridine 5′-triphosphate, Chinese hamster fibroblasts, enzyme kinetics, feedback inhibition, Ecology and Evolutionary Biology, Natural Resources and Environment, Molecular, Cellular and Developmental Biology, Science, Health Sciences
Language	English

Cited publications (2)

Pleiotropic mutants of Chinese hamster cells with altered cytidine 5′-triphosphate synthetase (1984)

Apparent synergism between amino donors for CTP synthesis in Chinese hamster fibroblasts (1983)