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Three myotropic peptides belonging to the Arg-amide insect tachykinin family were isolated from whole-body extracts of the mosquito, Culex salinarius . The peptides, APSGFMGMR-NH 2 , APYGFTGMR-NH 2 and APSGFFGMR-NH 2 (designated culetachykinin I, II, and III) were isolated and purified on the basis of their ability to stimulate muscle contractions of isolated Leucophaea maderae hindgut. Biologically inactive methionine sulfoxides of two of the three peptides were isolated using an ELISA system based upon antiserum raised against APYGFTGMR-NH 2 and identified with mass spectrometry. Immunocytochemistry localized these peptides in cells in the brain, antennae, subesophageal, thoracic and abdominal ganglion, proventriculus and midgut. Nerve tracts containing these peptides were found in the median nerve of the brain, central body, nervi corpus cardiaci, cervical nerve, antennal lobe and on the surface of the midgut..
Peer Reviewed.
http://deepblue.lib.umich.edu/bitstream/2027.42/45417/1/11064_2004_Article_419298.pdf |
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http://hdl.handle.net/2027.42/45417
http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=9475514&dopt=citation |
| Publisher |
Kluwer Academic Publishers-Plenum Publishers; Plenum Publishing Corporation ; Springer Science+Business Media
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| Contributors |
Department of Biological Chemistry and Biology, University of Michigan, Ann Arbor, MI, 48109-1048,
Food Animal Protection Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, 2881 F & B Road, College Station, TX, 77845,
Food Animal Protection Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, 2881 F & B Road, College Station, TX, 77845,
Food Animal Protection Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, 2881 F & B Road, College Station, TX, 77845,
Food Animal Protection Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, 2881 F & B Road, College Station, TX, 77845,
Zoological Institute, Catholic University of Leuven, Naamsestraat 59, B-3000, Leuven, (Belgium),
Department of Entomology, Institute for Biosciences and Technology, Texas A & M University, College Station, TX, 77843,
Department of Entomology, Texas Agricultural Experiment Station, Texas A & M University, College Station, TX, 77843,
Department of Entomology, Institute for Biosciences and Technology, Texas A & M University, College Station, TX, 77843,
Food Animal Protection Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, 2881 F & B Road, College Station, TX, 77845,
Ann Arbor
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| Repository |
University of Michigan (United States) |
| Keywords |
Biomedicine,
Peptide identification,
neuromodulators,
myotropins,
Neurosciences,
Neurology,
Biochemistry, general,
insect tachykinins,
homology,
Public Health,
Psychology,
Neurosciences,
Molecular, Cellular and Developmental Biology,
Internal Medicine and Specialties,
Biological Chemistry,
Social Sciences,
Science,
Health Sciences
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| Language |
English
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