| Gene alteration of intestinal intraepithelial lymphocytes in response to massive small bowel resection (2003) | |||||||||||||||
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| Pediatr Surg Int DOI R A T Barbara Wildhaber Hua Yang Arnold Coran Daniel Teitelbaum Gene alteration intestinal intraepithelial lymphocytes response massive small bowel resection Accepted December Published online May Springer Verlag Abstract Background The intestinal adaptive response increased epithelial cell proliferation and apoptosis after massive small bowel resection SBR partially controlled intraepithelial lymphocytes IEL identify IEL factors contributing adaptation postSBR utilized microarray assays Methods Mice underwent SBR SBR SBR sham operation Sham Sham After weeks small bowel was harvested and IEL isolated Determination the proliferation rate used BrdU incorporation and the apoptotic rate used Annexin staining ymetrix system microarrays genes were performed examine IEL mRNA expression Results were considered signi cant foldchange between groups was and test and or and Signi cant genes were con rmed conventional PCR Results The SBR proliferation rate increased signi cantly both and groups compared Sham SBR Sham SBR Sham The apoptotic rate was unchanged the group but signi cantly ered from controls after weeks SBR Sham Microarray results were analyzed identify potential growth modifying IEL genes The following were identi function parenthesis apoptosis proliferation lipocalin promotes angiotensin converting enzyme increases Rap interacting protein reduces promotes amphiregulin promotes and leucine rich glycoprotein promotes reduces Based PCR results these genes s. Peer Reviewed. http://deepblue.lib.umich.edu/bitstream/2027.42/47154/1/383_2003_Article_1001.pdf | |||||||||||||||
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