| A rapid electrophoretic technique for human and Chinese hamster galactokinase (1975) | |||||||||||||||
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| Kumangenetik Springer Verlag Rapid Electrophoretic Technique for Human and Chinese Hamster Galactokinase Sun Chang and Chu Department Human Genetics University Michigan Medical School Ann Arbor Michigan A eceived May Summary this paper simple direct staining method described for the detection the human and Chinese hamster forms galactokinase separated electrophoresis Galactokinase plays key role the utilization galactose catalyzing the conversion galactose galactose phosphate gal the presence and adenosine triphosphate ATP The great bulk galactose metabolized appears follow the Leloir pathway Beutler and Morrison Galactokinase deficiency galactosemia man inherited autosomal recessive trait and results gastrointestinal dysfuction and jaundice the newborn period and the appearance cataracts the older patients unrestricted diets Gitzelmann Segal The gene for galactokinase has been recently assigned specific region the human chromosome Elsevier press The assignment the gene was made possible the development electrophoretic method for separating the homologous enzyme derived from human Chinese hamster and mouse tissues Nichols The technique based the precipitation galactose POt into the matrix the gel lanthanum chloride The gel then washed remove unreacted galaetose dried exposed ray film where the site enzyme activity demonstrated autoradiography The method appears sensitive but laborious and time consuming this paper describe simple direct staining method for the detection galac. Peer Reviewed. http://deepblue.lib.umich.edu/bitstream/2027.42/47602/1/439_2004_Article_BF00394199.pdf | |||||||||||||||
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