| Regional chromosomal localization of the human gene for galactose-1-phosphate uridyltransferase (1977) | |||||||||||||||
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| Hum Genet Springer Verlag Regional Chromosomal Localization the Human Gene for Galactose Phosphate Uridyltransferase Sun Sun and h Biology Division Oak Ridge National Laboratory Oak Ridge Tennessee USA Department Human Genetics University Michigan Medical School Ann Arbor Michigan USA Summary the progeny somatic cell hybrids formed fusion human lymphocytes and Chinese hamster mutant cells single human chromosome was selectively retained when grown appropriate medium Spontaneous breakage this chromosome different hybrid subclones led the assignment the gene for galactose phosphate uridyltransferase the centromeric region this chromosome qll This gene shown syntenic the previously mapped genes for acid phosphatase and malate dehydrogenase Introduction Somatic cell hybridization between rodent and human cells and subsequent preferential elimination human chromosomes from the resulting cell hybrids have provided powerful tool for assigning human genes individual chromosomes Taking this approach has been possible assign nearly genes all human chromosomes except and Kucherlapati and Ruddle and the number increasing Retaining specific human chromosome the hybrid progeny was made feasible method linkage ascertainment fixation first proposed Weiss and Green The technique requires the use mutant rodent cells lacking the capacity synthesize specific enzyme appropriate selective medium the hybrid cell may survive because the rodent mutant phenotype masked the wild type allele specific hu. Peer Reviewed. http://deepblue.lib.umich.edu/bitstream/2027.42/47607/1/439_2004_Article_BF00393609.pdf | |||||||||||||||
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