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Isolation of DNA sequences on human chromosome 21 by application of a recombination-based assay to DNA from flow-sorted chromosomes (1988)

Abstract

By merging two efficient technologies, bivariate flow sorting of human metaphase chromosomes and a recombination-based assay for sequence complexity, we isolated 28 cloned DNA segments homologous to loci on human chromosome 21. Subregional mapping of these DNA segments with a somatic cell hybrid panel showed that 26 of the 28 cloned DNA sequences are distributed along the long arm of chromosome 21, while the other 2 hybridize with sequences on the short arm of both chromosome 21 and other chromosomes. This new collection of probes homologous to chromosome 21 should facilitate molecular analyses of trisomy 21 by providing DNA probes for the linkage map of chromosome 21, for studies of nondisjunction, for chromosome walking in clinically relevant subregions of chromosome 21, and for the isolation of genes on chromosome 21 following the screening of cDNA libraries.. Peer Reviewed. http://deepblue.lib.umich.edu/bitstream/2027.42/47623/1/439_2004_Article_BF00366237.pdf

Publication details

Download	, http://hdl.handle.net/2027.42/47623 http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=3402991&dopt=citation
Publisher	Springer-Verlag
Contributors	Howard Hughes Medical Institute at the University of Michigan Medical Center, 48109, Ann Arbor, MI, USA, Howard Hughes Medical Institute at the University of Michigan Medical Center, 48109, Ann Arbor, MI, USA, Howard Hughes Medical Institute at the University of Michigan Medical Center, 48109, Ann Arbor, MI, USA, Genetics Division, The Children's Hospital, 300 Longwood Avenue, 02115, Boston, MA, USA; Mental Retardation Center, The Children's Hospital, 02115, Boston, MA, USA; Department of Pediatrics, Harvard Medical School, 02115, Boston, MA, USA, Eleanor Roosevelt Institute for Cancer Research, University of Colorado Health Sciences Center, 80206, Denver, CO, USA, Genetics Division, The Children's Hospital, 300 Longwood Avenue, 02115, Boston, MA, USA; Mental Retardation Center, The Children's Hospital, 02115, Boston, MA, USA; Department of Pediatrics, Harvard Medical School, 02115, Boston, MA, USA, Genetics Division, The Children's Hospital, 300 Longwood Avenue, 02115, Boston, MA, USA; Mental Retardation Center, The Children's Hospital, 02115, Boston, MA, USA; Department of Pediatrics, Harvard Medical School, 02115, Boston, MA, USA, Eleanor Roosevelt Institute for Cancer Research, University of Colorado Health Sciences Center, 80206, Denver, CO, USA, Genetics Division, The Children's Hospital, 300 Longwood Avenue, 02115, Boston, MA, USA; Mental Retardation Center, The Children's Hospital, 02115, Boston, MA, USA; Department of Pediatrics, Harvard Medical School, 02115, Boston, MA, USA; Howard Hughes Medical Institute at The Children's Hospital, 02115, Boston, MA, USA; Department of Genetics, Harvard Medical School, 02115, Boston, MA, USA, Genetics Division, The Children's Hospital, 300 Longwood Avenue, 02115, Boston, MA, USA; Mental Retardation Center, The Children's Hospital, 02115, Boston, MA, USA; Department of Pediatrics, Harvard Medical School, 02115, Boston, MA, USA, Ann Arbor
Repository	University of Michigan (United States)
Keywords	Biomedicine, Metabolic Diseases, Internal Medicine, Molecular Medicine, Human Genetics, Molecular, Cellular and Developmental Biology, Genetics, Biological Chemistry, Science, Health Sciences
Type	Original Investigations
Language	English