| Gossypol Accumulation and Morphogenesis in Cotton (G. hirsutum L.) Callus Culture (2006) | |||||
Abstract | |||||
| The objective of this study was to determine the influence of different plant hormones on gossypol accumulation in callus culture and morphogenesis of cotton (G.hirsutum.L). Conditions have been described for in vitro culturing of tissues from the seeds of two different types of cotton (glanded and glandless). The callus cultures were initiated using defined levels of Kinetin (Kn) and 6-Benzylaminopurine (BAP) and 2.4-dichlorophenoxyacetic acid (2:4-D) and 1-naphthaleneacetic Acid (NAA) and were then transferred on the same media and on media with no hormones also. The gossypol content of each callus cultures and different parts (root, stem and leaf) of aseptically grown (7 to 10 days old) seedlings and seeds was determined using HPLC for cotton plants. The different levels of auxin and cytokinin in the culture media led to differentiation and increased the accumulation of gossypol. | |||||
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