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Plasmid Isolation in Legionella pneumophila and Legionella-like Organisms. (2002)

Abstract
Agarose gel electrophoresis was employed to screen nine strains of Legionella-like bacteria and one strain of Legionella pneumophila for the presence of extrachromosomal deoxyribonucleic acid. Cryptic plasmids were found in three of the isolates examined. A purification protocol was devised which consolidates the central tenets of several existing isolation techniques into a single procedure. Plasmid molecular weights were determined to range from 46.6 to 59.8 megadaltons (Mdal). (Author)

Publication details
Contributors ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FREDERICK MD
Repository Defense Technical Information Center OAI-PMH Repository (United States)
Keywords MEDICINE AND MEDICAL RESEARCH, MICROBIOLOGY, *BACTERIA, *BACTERIAL DISEASES, ISOLATION, STRAINS(BIOLOGY), GENETICS, ELECTROPHORESIS, ANTIBIOTICS., *Legionella pneumophila, Plasmids
Language eng