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Role of the C Terminus of FtsK in Escherichia coli Chromosome Segregation

Abstract
FtsK is essential for Escherichia coli cell division. We report that cells lacking the C terminus of FtsK are defective in chromosome segregation as well as septation, often exhibiting asymmetrically positioned nucleoids and large anucleate regions. Combining the corresponding truncated ftsK gene with a mukB null mutation resulted in a synthetic lethal phenotype. When the truncated ftsK was combined with a minCDE deletion, chains of minicells were generated, many of which contained DNA. These results suggest that the C terminus of FtsK has an important role in chromosome partitioning.

Publication details
Download http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=107737
Publisher American Society for Microbiology
Repository PubMed Central (PMC3 - NLM DTD) (United States)
Keywords Genetics and Molecular Biology
Type Text
Language Englisch