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Genetic Variability Analysis among Clinical Candida spp. Isolates Using Random Amplified Polymorphic DNA (2004) |
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Abstract |
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The patterns of genetic variation of samples of Candida spp. isolated from patients infected with human immunodeficiency virus in Vitória, state of Espírito Santo, Brazil, were examined. Thirty-seven strains were isolated from different anatomical sites obtained from different infection episodes of 11 patients infected with the human immunodeficiency virus (HIV). These samples were subjected to randomly amplified polymorphic DNA (RAPD) analysis using 9 different primers. Reproducible and complex DNA banding patterns were obtained. The experiments indicated evidence of dynamic process of yeast colonization in HIV-infected patients, and also that certain primers are efficient in the identification of species of the Candida genus. Thus, we conclude that RAPD analysis may be useful in providing genotypic characters for Candida species typing in epidemiological investigations, and also for the rapid identification of pathogenic fungi. |
Publication details |
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Cited publications (14) |
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Candida albicans genotyping in studies with patients with AIDS developing resistance to fluconazole.
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Correlation of in vitro fluconazole resistance of Candida isolates in relation to therapy and symptoms of individuals seropositive for human immunodeficiency virus type 1.
- Cameron, M L,
- Schell, W A,
- Bruch, S,
- Bartlett, J A,
- Waskin, H A,
- Perfect, J R
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Comparative analysis of genetic variability among Candida albicans isolates from different geographic locales by three genotypic methods.
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Rapid identification and fingerprinting of Candida krusei by PCR-based amplification of the species-specific repetitive polymorphic sequence CKRS-1.
- Carlotti, A,
- Chaib, F,
- Couble, A,
- Bourgeois, N,
- Blanchard, V,
- Villard, J
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Comparison of four molecular typing methods for evaluating genetic diversity among Candida albicans isolates from human immunodeficiency virus-positive patients with oral candidiasis.
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Genotypic identification and characterization of species and strains within the genus Candida by using random amplified polymorphic DNA.
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Candida albicans strain delineation.
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New method for detection of Candida albicans in human blood by polymerase chain reaction.
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Use of random amplified polymorphic DNA as a typing method for Candida albicans in epidemiological surveillance of a burn unit.
- Robert, F,
- Lebreton, F,
- Bougnoux, M E,
- Paugam, A,
- Wassermann, D,
- Schlotterer, M,
- Tourte-Schaefer, C,
- Dupouy-Camet, J
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Computer-assisted methods for assessing strain relatedness in Candida albicans by fingerprinting with the moderately repetitive sequence Ca3.
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