| The role of surface free energy on the formation of hybrid bilayer membranes (2002) | |||||||||||
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| The Role Surface Free Energy the Formation Hybrid Bilayer Membranes Vitalii Silin Herbert Wieder John Woodward Gintaras Valincius Offenhausser and Anne Plant Contribution from the National Institute Standards and Technology Biotechnology DiVision Gaithersburg Maryland FRIZ Biochem GmbH Martinsried Germany and Institute for Thin Films and Interfaces Research Center Juelich Juelich Germany Received April Abstract The interaction small phospholipid vesicles with well characterized surfaces has been studied assess the effect the surface free energy the underlying monolayer the formation phospholipid alkanethiol hybrid bilayer membranes HBMs The surface free energy was changed systematic manner using single component alkanethiol monolayers and monolayers binary mixtures thiols The binary surfaces were prepared gold self assembly from binary solutions the thiols X CH THF Surface plasmon resonance SPR electrical capacitance and atomic force microscopy AFM measurements were used characterize the interaction palmitoyl oleoyl phosphati dylcholine POPC vesicles with the surfaces For all surfaces examined appears that the polar part surface energy influences the nature the POPC assembly that associates with the surface Comparison optical capacitance and AFM data suggests that vesicles can remain intact partially intact even surfaces with contact angle with water close addition comparison the alkanethiols different chain lengths and the fluorinated compound CF that characterize with low v | |||||||||||
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