| Micropatterned substrates for the growth of functional neuronal networks of defined geometry (2003) | |||||||||||
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| Biotechnol Prog Micropatterned Substrates for the Growth Functional Neuronal Networks Defined Geometry Angela Vogt Lars Lauer Wolfgang Knoll and Andreas Offenhausser Max Planck Institute for Polymer Research Ackermannweg Mainz Germany and Institute Thin Films and Interfaces Bio and Chemosensors ISG Forschungszentrum Julich Julich Germany The vitro assembly neuronal networks with control over cell position and connectivity fascinating approach not only for topics basic neuroscience research but also diverse applications such biosensors and tissue engineering grew rat embryonic cortical neurons patterned substrates created microcontact printing Polystyrene was used cell repellent background onto which grid pattern physiological proteins was applied printed laminin and mixture extracellular matrix proteins and additionally both systems mixed with polylysine Attachment cells the pattern with high fidelity well the formation chemical synapses between neighboring cells the pattern could observed all four cases but cell attachment was strongly increased samples containing polylysine Neurons grown patterned substrates had membrane capacity smaller than that neurons homogeneously coated controls which attributed the geometrical restrictions but did not differ either resting membrane potential the quality synapses they formed therefore believe that the cells attach and differentiate normally the pattern and form functional mature synapses following the predefined geometry Introduction | |||||||||||
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