| Surface plasmon microscopy measurements of lipid vesicle adsorption on a micropatterned self-assembled monolayer (2001) | |||||||||||
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| Surface Plasmon Microscopy Measurements Lipid Vesicle Adsorption Micropatterned Self Assembled Monolayer Toby Jenkins Thomas Neumann and Andreas Offenha usser Max Planck Institut Polymerforschung Ackermannweg Mainz Germany Received December Final Form May This paper presents novel method measuring the adsorption egg phosphatidylcholine egg vesicles onto micropatterned heterogeneous self assembled monolayer SAM Microcontact printing was used make array mercaptoethanol patches surrounded octadecanethiol ODT SAM Surface plasmon microscopy SPM followed the change the different plasmon minima the mercaptoethanol and ODT SAMs with respect lipid adsorption time Gray scale analysis GSA the two domains showed clear differences the kinetics lipid adsorption which were equated very different mechanism lipid vesicle fusion microcontactprinted SAMscompared with homogeneous SAMs Surface plasmon microscopy combined with GSA has allowed spatially deconvolute the adsorption lipid patterned surface giving unique insight into the lipid adsorption process Conventional surface plasmon resonance measurements were made the homogeneous SAM moieties order quantify the gray scale analysis terms adsorbed lipid thickness Introduction The theory and background surface plasmon croscopy SPM has been well documented Knoll simple terms can understood considering how the angular position the plasmon resonance minimum created the interaction incident laser light with thinmetalfilm ismediatedbythethicknessandre | |||||||||||
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