| Scanning Probe Microscopic Studies of the Oriented Attachment and Membrane Reconstitution of Cytochrome c Oxidase to a Gold Electrode (2005) | |||||||||||
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| Langmuir Scanning Probe Microscopic Studies the Oriented Attachment and Membrane Reconstitution Cytochrome Oxidase Gold Electrode Dirk Mayer Kenichi Ataka Joachim Heberle and Andreas Offenhausser Research Center Juelich ISG Institute for Bio and Chemosensors IBI Structural Biology Juelich Germany Received May Final Form July Scanning probe microscopy was used monitor the resulting surface the oriented incorporation cytochrome oxidase into electrode supported lipid bilayer four crucial stages with molecular resolution were able reveal the formation densely packed monolayer the active ester dithio succiniimidylepropionate DTSP and the covalent linkage the nitrilotriacetic acid NTA the thiol anchored DTSP scanning tunneling microscopy Atomic force microscopy investigations showed that the detergent solubilized oxidase immobilized monomers and small aggregates via histidine residues Finally the reconstitution the proteins within the supported membrane was verified The amount oxidase immobilized within the solid supported membrane was estimated Introduction Many the vital functions cells are maintained membrane proteins which for instance selectively control the transfer ions functional agents and energy between the periplasm and cytoplasm cell Often certain function accomplished the complex interplay number different proteins supported defined environment The high complexity biological transmembrane machineries with respect their structure the reduced periodicity and the multiste | |||||||||||
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