| Cell-transistor coupling: Investigation of potassium currents recorded with p- and n-channel FETs (2005) | |||||||||||
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| Biophysical Journal Volume November Cell Transistor Coupling Investigation Potassium Currents Recorded with and Channel FETs Gunter Wrobel Reinhard Seifert Sven Ingebrandt Jorg Enderlein Holger Ecken Arnd Baumann Benjamin Kaupp and Andreas Offenhausser Institute Thin Films and Interfaces ISG and Center Nanoelectronic Systems for Information Technology and yInstitute Biological Information Processing IBI Research Centre Julich Julich Germany ABSTRACT Microelectronic based biosensors that allow noninvasive measurement cell activity are the focus current developments however the mechanisms underlying the cell transistor coupling are not completely understood particular characteristic properties the extracellular voltage response such the waveform and amplitude are not satisfactorily described electrical circuit models Here examine the electrical coupling between nonmetallized eld effect transistor FET and cell line expressing voltage gated EAG channel The activation kinetics this channel depends the voltage pulse protocol and extracellular divalent cations This feature allows testing whether the extracellular voltage signal recorded with the FET faithfully tracks the current simultaneously recorded with the patch clamp technique that the FET signals contain different kinetic components that cannot entirely explained equivalent electrical circuit models Rather suggest that changes ion concentration the small cleft between cell and FET may change the surface potential the FET This | |||||||||||
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Cited publications (5) | |||||||||||