| Membrane on a Chip. A functional tethered lipid bilayer membrane on silicon oxide surface (2005) | |||||||||||
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| Biophysical Journal Volume September Membrane Chip Functional Tethered Lipid Bilayer Membrane Silicon Oxide Surfaces Vladimir Atanasov Nikolaus Knorr Randolph Duran Sven Ingebrandt Andreas Offenhausser Wolfgang Knoll and Ingo Koper Max Planck Institute for Polymer Research Mainz Germany yDepartment Chemistry University Florida Gainesville Florida and zResearch Center Julich Julich Germany ABSTRACT Tethered membranes have been proven during recent years powerful and exible biomimetic platform reported previous article the design new architecture based the self assembly thiolipid ultrasmooth gold substrates which shows extremely good electrical sealing properties well functionality bilayer membrane Here describe the synthesis lipids for more modular design and the adaptation the linker part silane chemistry were able form functional tethered bilayer lipid membrane with good electrical sealing properties covering silicon oxide surface demonstrate the functional incorporation the ion carrier valinomycin and the ion channel gramicidin INTRODUCTION Solid supported membrane systems were used the last decade variety ways mimic properties natural membrane Different approaches have been used either creating bilayer directly surface using polymer cushions protein layers spacers The investigated surfaces were either glass silicon substrates metal electrodes gold The advantage using spacer between substrate and bilayer the fact that this construct provides ionic reservoir underneath the m | |||||||||||
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