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Preparation of Protoplasts from Chlorella vulgaris K-73122 and Cell Wall Regeneration of Protoplasts from C. vulgaris K-73122 and C-27 (2003) |
- Honjoh, Ken-ichi,
- Suga, Koushirou,
- Shinohara, Fuminori,
- Maruyama, Isao,
- Miyamoto, Takahisa,
- Hatano, Shoji,
- Iio, Masayoshi,
- 本城, 賢一,
- 菅, 向志郎,
- 篠原, 史訓,
- 丸山, 功,
- 宮本, 敬久,
- 波多野, 昌二,
- 飯尾, 雅嘉,
- ホンジョウ, ケンイチ,
- スガ, コウシロウ,
- シノハラ, フミノリ,
- マルヤマ, イサオ,
- ミヤモト, タカヒサ,
- ハタノ, ショウジ,
- イイオ, マサヨシ
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Abstract |
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Protoplasts from Chlorella vulgaris K-73112 were obtained by enzymatic digestion with a mixture of Acromopeptidase, Cellulase ONOZUKA R-10, Chitosanas Kl, Gluczyme, and Uskizyme. The formation of naked protoplasts was confirmed by fluorescence microscopy using fluorescent brightner 28, which stains cell walls. About 88% of C. vulgaris K-73112 cells were covereted into osmotically-labile cells. Futhermore, a method for regeneration of intact cells from the protoplasts was developed. Utilization of 0.5M sucrose as an osmoticum, Fe-EDITA as an iron source, and bacto-agar as a supporting was shown to help regeneration of the cell walls of two strains, C. vulgaris K-73112 and C-27. |
Publication details |
| Download |
http://hdl.handle.net/2324/4494 |
| Publisher |
Faculty of Agriculture, Kyushu University,
九州大学大学院農学研究院
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| Contributors |
Laboratory of Food Hygienic Chemistry, Division of Food Biotechnology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University[Honjoh,Suga,Shinohara],
Chlorella Industries Co.Ltd.[Maruyama],
Graduate School if Healts and Social Welfare Science, Nishikyushu University[Hatano]
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| Repository |
Kyushu University Institutional Repository(QIR) (Japan) |
| Type |
紀要論文,
Departmental Bulletin Paper
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| Language |
English
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| Relation |
http://www.agr.kyushu-u.ac.jp/index.html |
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