| Variabilities of the impacts of cold pathogen and cold-dampness pathogen on fractalkine mRNA expression in lung tissues of rats (2300) | |||||||||
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| Objective: To study the variabilities of the effects of cold pathogen and cold-dampness pathogen on the fractalkine (FKN) mRNA expression in lung tissues of rats.Methods: Twenty-four Wistar rats of SPF grade were randomly divided into 3 groups: normal temperature group, cold pathogen group and cold-dampness pathogen group. There were 8 rats in each group. Rats in normal temperature group were bred at (20±2)℃ and under normal humidity (50％-55％) for 2 h. Rats in cold pathogen group were bred at －10℃ and under normal humidity (50％-55％) for 2 h, and the rats in cold-dampness pathogen group were bred at －10℃ and under high humidity (90％-100％) for 2 h. Rats in the three groups were bred in thermostats under the corresponding conditions on the first day of experiment, and then the rats in different groups were all bred at normal temperature. Lung specimens in 3 groups were gathered four days later. The behavior and the pathological changes in the lung tissues of rats in different groups were observed. The content of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in lung homogenate was detected by radioimmunoassay (RIA) method. Expression of FKN mRNA in lung homogenate was detected by reverse transcription-polymerase chain reaction (RT-PCR).Results: The lung tissues of rats in both cold pathogen group and cold-dampness pathogen group had various degrees of pathological changes. Compared with normal temperature group, the content of IL-6 and TNF-α was increased obviously in lung homogenate of rats in both cold pathogen group and cold-dampness pathogen group (P | |||||||||
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