| Golgi structure in three dimensions: functional insights from the normal rat kidney cell (1999) | |||||||||||||||
Abstract | |||||||||||||||
| Abstract. Three-dimensional reconstructions of portions of the Golgi complex from cryofixed, freeze-substituted normal rat kidney cells have been made by dual-axis, high-voltage EM tomography at �7-nm resolution. The reconstruction shown here ( �1 � 1 � 4 3 �m) contains two stacks of seven cisternae separated by a noncompact region across which bridges connect some cisternae at equivalent levels, but none at nonequivalent levels. The rest of the noncompact region is filled with both vesicles and polymorphic membranous elements. All cisternae are fenestrated and display coated buds. They all have about the same surface area, but they differ in volume by as much as 50%. The transmost | |||||||||||||||
Publication details | |||||||||||||||
| |||||||||||||||