| Physical Mapping Assembling Physical Maps by (2008) | |||||||||||||||
Abstract | |||||||||||||||
| Genetic mapping is only the first step toward positional cloning of a gene. Once a gene has been determined to lie between two genetic markers, the geneticist must produce a physical map—consisting of overlapping clones spanning the chromosomal region between the two flanking markers. Traditionally, physical maps have been produced by the process of chromosomal walking: one starts with clone C1 containing one of the genetic markers, uses C1 as a probe to find an overlapping clone C2, uses C2 as a probe to find C3, and so on until the region has been spanned (Figure 1). Chromosomal walking is an inherently serial procedure, and each | |||||||||||||||
Publication details | |||||||||||||||
| |||||||||||||||