| Quantitative analysis of survivin mRNA expression in bladder transitional cell carcinomas (2008). 膀胱移行上皮癌患者における半定量的サバイビンmRNA測定法の検討 | |||||||||||||||
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| 膀胱移行上皮癌患者におけるサバイビンmRNA発現の診断・予後因子としての有用性を検討した。リアルタイムPCR法を用い膀胱移行上皮癌患者21例の膀胱癌組織と初発例12例の自排尿中のサバイビンmRNA発現量を半定量的に測定し, すべての腫瘍組織と12例中7例の自排尿中にサバイビンmRNAの発現を認めた。サバイビンmRNAの発現量は低分化癌および浸潤性腫瘍で有意に高値であった。表在性膀胱癌症例のうちサバイビンmRNAの発現量が高い3例で再発を認めた。浸潤性膀胱癌症例ではサバイビンmRNAの発現量と局所再発あるいは遠隔転移とは関係がなかった。これらのことより膀胱移行上皮癌患者におけるサバイビンmRNAの半定量的測定は膀胱内再発についての悪性度を示している可能性が考えられた。(著者抄録). We assessed the expression levels of survivin mRNA in bladder transitional cell carcinoma (TCC) to provide additional information regarding its malignant potential. The real-time PCR method was used to detect the survivin mRNA level for 21 bladder tumor specimens, and for urinary exfoliated cells from 12 newly diagnosed bladder tumor patients. All bladder tumor specimens and 7 of 12 voided urine specimens expressed survivin mRNA. In tumor specimens, high grade, high stage tumors had the tendency to express more survivin mRNA. Of 12 superficial bladder tumor patients who had transurethral resection of bladder tumor (TURB), 3 showed high survivin mRNA expression and intravesical recurrence after the surgery. However, for the patients who had total cystectomy due to invasive tumor, no relations were observed between the survivin mRNA expression level and development of local recurrence and/or distant metastasis. Our results suggested that the quantitative analysis of the survivin mRNA may indicate local malignant potential, which contribute to the possibility of an intravesical recurrence. | |||||||||||||||
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