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Aerosol gene transfer with inducible nitric oxide synthase reduces hypoxic pulmonary hypertension and pulmonary vascular remodeling in rats (2000)

Abstract

BACKGROUND: Nitric oxide (NO) is a potent vasodilator with an important role in the regulation of pulmonary vascular tone. The effects of NO synthase (NOS) gene transfer on pulmonary vascular remodeling associated with hypoxic pulmonary hypertension are unknown. METHODS AND RESULTS: We aerosolized 3 x 10(9) pfu of an adenoviral vector containing inducible NOS gene (AdNOS2), constitutive NOS3 gene (AdNOS3), or no transgene (AdRR5) into rat lungs. Exhaled NO levels, monitored with chemiluminescence, were higher in AdNOS2-infected rats than in AdNOS3- and AdRR5-infected rats (at 3 days, 33+/-6 ppb, n=9, versus 17+/-4, n=9, and 6+/-2 ppb, n=3, P:. Department of Cardiology, Flanders Interuniversity Institute of Biotechnology, University of Leuven, Belgium.

Publication details

Download	http://circ.ahajournals.org/cgi/pmidlookup?view=long&pmid=11104748
Publisher	Lippincott williams & wilkins
Repository	Lirias is a research document repository at KULeuven (Belgium)
Keywords	nitric oxide synthase, hypertension, pulmonary, genes, vasculature, remodeling, right-ventricular hypertrophy, structural-changes, inhaled no, lungs, circulation, relaxation, expression, arteries, growth, vasoconstriction, Aerosols, Animals, Anoxia, Disease Models, Animal, Endothelium, Vascular, Gene Transfer Techniques, Genetic Vectors, Hypertension, Pulmonary, Male, Nitric Oxide Synthase, Nitric Oxide Synthase Type II, Pulmonary Artery, Pulmonary Circulation, Pulmonary Veins, Rats, Rats, Wistar, Transgenes
Type	Description (Metadata) only, IT, article
Language	English
Relation	Circulation vol:102 issue:23 pages:2880-2885