| Generation of "backbone" free, low transgene copy plants by launching T-DNA from the _Agrobacterium_ chromosome (2009) | |||||||||
Abstract | |||||||||
| In both applied and basic research, _Agrobacterium_-mediated transformation is commonly used to introduce genes into plants. We investigated the effect of three _Agrobacterium tumefaciens_ strains and five T-DNA origins of replication on transformation frequency, transgene copy number, and the frequency of integration of non-T-DNA portions of the T-DNA-containing vector ("backbone") into the genome of _Arabidopsis thaliana_ and _Zea mays_. Launching T-DNA from the _picA_ locus of the _Agrobacterium_ chromosome increases the frequency of single transgene integration events and almost eliminates the presence of vector backbone sequences in transgenic plants. Along with novel _Agrobacterium_ strains we have developed, our findings are useful for improving the quality of T-DNA integration events. | |||||||||
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