| Roles of Inorganic Nitrogen in Gametophytic Growth and in Initiation and Development of Sporophytic Shoots of Equisetum arvense (1992) | |||||||||||||
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| The formation of sporophytic shoots, which is induced by treatment with benzylaminopurine of gametophyte tissue of Equisetum arvense, can be divided into initiation and developmental phases. The nitrogen in MS medium $$\left({\hbox{ NH }}_{3}^{-}:{\hbox{ NH }}_{4}^{+}=2:1\right)$$ was suitable for two phases as well as gametophytic growth, but a reduction in the concentration of available nitrogen was needed for the development of shoots. $${\hbox{ NO }}_{3}^{-}$$ ions alone were effective for gametophytic growth and the initiation of sporophytic shoots, but both $${\hbox{ NO }}_{3}^{-}$$ and $${\hbox{ NH }}_{4}^{+}$$ ions was required for the developmental phase. | |||||||||||||
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