| RNA-primed intitiation sites of DNA replication in the origin region of bacteriophage {lambda} genome (1988) | |||||||||||||
Abstract | |||||||||||||
| Using DNA molecules synthesized in the early stage of λ phage infection, deoxynucleotides at the transition sites from primer RNA to DNA synthesis have been mapped in the 1.5 kbase area of the λ phage genome containing the genetically defined replication origin (oriλ). Sites in the 1-strand (the polarity of the 1-strand is 5′ to 3′ from the left to the right direction of the λ phage genetic map) were distributed both inside and outside of the oriλ, whereas the sites in the r-strand (the strand in the opposite polarity) were mainly distributed more than three hundred nueleotides apart from the oriλ to the right. A CPuPu sequence was found at −12 to −10 region of transition sites of the r- and the 1-strands in the frequency of 80% and 70%, respectively, and over 60% of the CPuPu sequences were CAG. Properties of the transition sites are discussed in relation to the primer synthesis. | |||||||||||||
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