| Cloning of cDNA encoding a 100 kDa nudeolar protein (nucleoline) of Chinese hamster ovary cells (1985) | |||||||||||||
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| Nucleoline (100 kDa) is the major nucleolar protein in exponentially growing cells that behaves like a nucleolar organizer protein and plays a key role in rDNA transcription and prerRNA processing. We reported the isolation of 5 cDNA clones by probing a cDNA library, constructed in the expression vector λ gt11, with a polyclonal serum raised against nudeoline. A new immunoassay, using hybrid proteins (βgal-cDNA encoded protein)was developped to establish that the isolated cDNAs encoded parts of nucleoline. A further confirmation resulted from the sequence comparison between the cDNA encoded peptide and a 42 aa peptide isolated from rat nudeoline (1). The 5 cDNAs overlapped extensively and covered more than 90 % of a full length cDNA. By probing a Northern blot with the 100kDa cDNA, a 2650 nucleotide polyA+ RNA was detected that contained just enough information to code for nucleoline. | |||||||||||||
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