| Localization and DNA sequence analysis of the C gene of bacteriophage Mu, the positive regulator of Mu late transcription (1986) | |||||||||||||
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| The C gene of bacteriophage Mu, required for transcription of the phage late genes, was localized by construction and analysis of a series of deleted derivatives of pKN50, a plasmid containing a 9.4 kb Mu DNA fragment which complements Mu C amber mutant phages for growth. One such deleted derivative, pWMl0, containing only 0.5 kb of Mu DNA, complements C amber phages and transactivates the mom gene, one of the Mu late genes dependent on C for activation. The DNA sequence of the 0.5 kb fragaent predicts a single long open reading frame coding for a 140 amino acid protein. Sequence analysis of DNA containing a C amber nutation located the base change to the second codon of this reading frame. Generation of a frameshift mutation by filling in a BkIII site spanning codon 114 of this reading frame resulted in the loss of C complementation and transactivation activity. These results indicate that this open reading frame encodes the Mu C gene product. Comparinon of the predicted amino acid sequence of the C protein with those of other transcriptional regulatory proteins revealed some similarity to a region highly conserved among bacterial slgma factors. | |||||||||||||
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