| Alkali-labile structures linked to the 5' ends of Bacillus subtilis short DNA chains (1985) | |||||||||||||
Abstract | |||||||||||||
| Alkali-labile portion covalently linked to the 5′ ends of Bacillus subtilis short DNA chains, the putative primer RNA for discontinuous DNA synthesis, was isolated and analyzed using a temperature sensitive DNA polymerase I mutant, which accumulates nascent DNA fragments at a restrictive temperature. A novel oligonucleotide structure as well as mono- to tri- ribonucleotide stretches were isolated at the 5′ end of the short, DNA chains. A structure for the novel oligonucleotide is proposed to be p5′ X3′ pp5′ rN, where X represents unidentified nucleoside with a peculiar property. Possible metabolic relationship between these molecules and primer RNA has been discussed. | |||||||||||||
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