| Genome Informatics 14: 458--459 (2003) Characterization of One-Pass and Full-Length Sequences of Oligo-Capping cDNA Clones by Genome Mapping (2004) | |||||||||||||||
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| Introduction Whole human genome sequence determination was completed this year. To obtain accurate gene structures and promoter information, full-length cDNA sequence information as well as the genome sequences is indispensable. Moreover, the full-length cDNA clones and sequences are valuable for functional analysis of genes. The oligo-capping cDNA library developed by Maruyama and Sugano is an e#ective source of the full-length cDNA clones. The full-length human cDNA sequencing project [2] supported by New Energy and Industrial Technology Developmental Organization (NEDO) determined 30,000 full-length and more than one million 5' one pass cDNA sequences of oligo-capping clones obtained at HRI and Tokyo University. Consequently, the NEDO human splicing variant cDNA project started in 2002, taking advantage of these oligo-capping cDNAs which are regarded as a valuable source of splicing variant cDNAs. Several large scale splicing variant studies by mapping EST sequences to genome sequ | |||||||||||||||
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