| Pseudo-immunolabelling with the avidin-biotin-peroxidase complex (ABC) due to the presence of endogenous biotin in retinal Muller cells of goldfish and salamander (1997) | |||||||||
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Abstract | |||||||||
| Journal Neuroscience Methods Pseudo immunolabelling with the avidin biotin peroxidase complex ABC due the presence endogenous biotin retinal Muller cells gold and salamander Josobanta Bhattacharjee Bob Nunes Cardozo Willem Kamphuis Maarten Kamermans Gijs Vrensen a Graduate School Neurosciences Amsterdam The Netherlands The Netherlands Ophthalmic Research Institute Box Amsterdam The Netherlands Department Zoology Egerton Uni ersity Box Njoro Kenya Department Medical Physics Uni ersity Amsterdam Amsterdam The Netherlands Received February accepted June Abstract Immunodetection techniques are dependent enzyme protein conjugates for the visualisation antigen antibody complexes One the most widely used the avidin biotin peroxidase complex ABC method The present study demonstrates that direct treatment gold and salamander retinal sections with ABC followed incubation with the chromogenic substrate diaminobenzidine tetrahydrochloride DAB and manifested punctate staining pattern across the neural retinae presumably through binding avidin endogenous biotin Incubation with primary antiserum against biotin followed immunoprocessing with the peroxidase anti peroxidase PAP method showed pattern similar the punctuate framework detected with solo ABC treated sections Moreover the ABC DAB mediated pattern corresponded the spatial orientation Muller cells identi GFAP immunostaining These ndings indicate the presence endogenous biotin Muller cells and calls for caution the application the ABC | |||||||||
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