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Enrichment of measles virus-like RNA in the nucleocapsid fraction isolated from subacute sclerosing panencephalitis brains (1982)

Abstract

A procedure has been developed which facilitates the detection of measles virus RNA sequences in human brains. The procedure involves isolating subviral components (nucleocapsids) from brain tissues prior to RNA purification, followed by hybridization of these RNAs to cDNA synthesized from measles virus 50 S RNA template. Using these techniques we were able to obtain an RNA fraction which was manyfold enriched in measles virus-specific RNA, relative to unfractionated subacute sclerosing panencephalitis (SSPE) brain RNAs. 70-100% of the measles virus-specific RNA present in these SSPE brain samples were recovered in this enriched fraction.. Peer Reviewed. http://deepblue.lib.umich.edu/bitstream/2027.42/24061/1/0000313.pdf

Publication details

Download	, http://www.sciencedirect.com/science/article/B6T96-476W86X-1P/2/1d33dbb6075fd6b740341d41b4f5842f http://hdl.handle.net/2027.42/24061 http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=retrieve&db=pubmed&list_uids=6895898&dopt=citation http://dx.doi.org/10.1016/0166-0934(82)90052-0
Publisher	Elsevier
Contributors	Departments of Epidemiology and Anaesthesiology, School of Public Health and School of Medicine, University of Michigan, Ann Arbor, MI 48109, U.S.A., Professor of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, MI 48109, U.S.A., Center for Neurologic Study, 11575 Sorrento Valley Road, Suite 203, San Diego, CA 92121, U.S.A., Chief, Neurology Service V.A. Wadsworth Hospital Center, Wilshire and Sawtelle Boulevards, Los Angeles, CA 90073, U.S.A.
Repository	University of Michigan (United States)
Keywords	Public Health, Biological Chemistry, Science, Health Sciences
Language	English

Cited publications (1)

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