A. Bacher

The Fluorescence Detector of the Pierre Auger Observatory (2009)

Abreu, P., Aglietta, M., Aguirre, C., Ahn, E. J., Allard, D., ...

The Pierre Auger Observatory is a hybrid detector for ultra-high energy cosmic rays. It combines a surface array to measure secondary particles at ground level together with a fluorescence detector...

Structure of lumazine protein, an optical transponder of luminescent bacteria (2008)

Chatwell, L., Illarionova, V., Illarionov, B., Eisenreich, W., Huber, R., Skerra, A., ...

Biosynthesis of riboflavin: Structure and properties of 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5 '-phosphate reductase of Methanocaldococcus jannaschii (2006)

Chatwell, L., Krojer, T., Fidler, A., Romisch, W., Eisenreich, W., Bacher, A., ...

Crystal structure of an archaeal pentameric riboflavin synthase in complex with a substrate analog inhibitor - Stereochemical implications (2006)

Ramsperger, A., Augustin, M., Schott, A. K., Gerhardt, S., Krojer, T., Eisenreich, W., ...

Photometry from online Digitized Sky Survey Plates (2005)

Bacher, A., Kimeswenger, S., Teutsch, P.

Online Digital Sky Survey (DSS) material is often used to obtain information on newly discovered variable stars for older epochs (e.g. Nova progenitors, flare stars, ...). We present here the results...

Les pesticides issus de la viticulture dans les cours d'eau : des traitements aux impacts sur la macrofaune aquatique (2005)

Bacher, A.

/ Ce rapport fait état dans premier temps de l'historique des produits phytosanitaires afin de comprendre la problématique des pesticides (leurs modes d'action, leur classification et leur...

Réponse des communautés d'invertébrés benthiques d'un cours d'eau du Beaujolais à un gradient de contamination par les pesticides (2005)

Bacher, A.

The catchment area of Morcille is covered by 60% of vineyard. The purpose of this study is to assess the response of the benthic invertebrates to pollution by pesticides (fungicidal, weedkillers,...

Usages des sols et qualité écologique des milieux aquatiques : vignoble et communautés d'invertébrés benthiques (2005)

Roger, M.C., Bacher, A., Gouy, V., Montuelle, B.

/ Les zones rurales peuvent causer des pollutions des milieux aquatiques récepteurs en fonction de l'usage des sols et des pratiques agricoles. Les zones de vignoble sont des milieux où...

Crystal structures of the antitermination factor NusB from Thermotoga maritima and implications for RNA binding (2004)

Bonin,I., Robelek,R., Benecke,H., Urlaub,H., Bacher,A., Richter,G., ...

Evolution of vitamin B-2 biosynthesis. A novel class of riboflavin synthase in Archaea (2004)

Fischer,M., Schott,A. K., Romisch,W., Ramsperger,A., Augustin,M., Fidler,A., ...

Structural basis for the interaction of Escherichia coli NusA with protein N of phage lambda (2004)

Bonin,I., Muhlberger,R., Bourenkov,G. P., Huber,R., Bacher,A., Richter,G., ...

Potential anti-infective targets in pathogenic yeasts: Structure and properties of 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans (2004)

Echt,S., Bauer,S., Steinbacher,S., Huber,R., Bacher,A., Fischer,M.

Structural basis of charge transfer complex formation by riboflavin bound to 6,7-dimethyl-8-ribityllumazine synthase (2004)

Koch,M., Breithaupt,C., Gerhardt,S., Weber,S., Cushman,M., Huber,R., ...

Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways (2004)

Laupitz,R., Hecht,S., Amslinger,S., Zepeck,F., Kaiser,J., Richter,G., ...

Metal sites in 3,4-dihydroxy-2-butanone 4-phosphate synthase from Methanococcus jannaschii in complex with the substrate ribulose 5-phosphate (2004)

Steinbacher,S., Schiffmann,S., Bacher,A., Fischer,M.

Biosynthesis of tetrahydrofolate in plants: Crystal structure of 7,8-dihydroneopterin aldolase from Arabidopsis thaliana reveals a novel adolase class (2004)

Bauer,S., Schott,A. K., Illarionova,V., Bacher,A., Huber,R., Fischer,M.

Structure of the molybdenum-cofactor biosynthesis protein MoaB of Escherichia coli (2004)

Bader,G., Gomez-Ortiz,M., Haussmann,C., Bacher,A., Huber,R., Fischer,M.

Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways (2004)

Laupitz, R., Hecht, S., Amslinger, S., Zepeck, F., Kaiser, J., Richter, G., ...

Biosynthesis of tetrahydrofolate in plants: Crystal structure of 7,8-dihydroneopterin aldolase from Arabidopsis thaliana reveals a novel adolase class (2004)

Bauer, S., Schott, A. K., Illarionova, V., Bacher, A., Huber, R., Fischer, M.

Crystal structures of the antitermination factor NusB from Thermotoga maritima and implications for RNA binding (2004)

Bonin, I., Robelek, R., Benecke, H., Urlaub, H., Bacher, A., Richter, G., ...

Evolution of vitamin B-2 biosynthesis. A novel class of riboflavin synthase in Archaea (2004)

Fischer, M., Schott, A. K., Romisch, W., Ramsperger, A., Augustin, M., Fidler, A., ...

Metal sites in 3,4-dihydroxy-2-butanone 4-phosphate synthase from Methanococcus jannaschii in complex with the substrate ribulose 5-phosphate (2004)

Steinbacher, S., Schiffmann, S., Bacher, A., Fischer, M.

Potential anti-infective targets in pathogenic yeasts: Structure and properties of 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans (2004)

Echt, S., Bauer, S., Steinbacher, S., Huber, R., Bacher, A., Fischer, M.

Structural basis for the interaction of Escherichia coli NusA with protein N of phage lambda (2004)

Bonin, I., Muhlberger, R., Bourenkov, G. P., Huber, R., Bacher, A., Richter, G., ...

Structural basis of charge transfer complex formation by riboflavin bound to 6,7-dimethyl-8-ribityllumazine synthase (2004)

Koch, M., Breithaupt, C., Gerhardt, S., Weber, S., Cushman, M., Huber, R., ...

Structure of the molybdenum-cofactor biosynthesis protein MoaB of Escherichia coli (2004)

Bader, G., Gomez-Ortiz, M., Haussmann, C., Bacher, A., Huber, R., Fischer, M.

Crystal structures of the antitermination factor NusB from Thermotoga maritima and implications for RNA binding (2004)

Bonin, I., Robelek, R., Benecke, H., Urlaub, H., Bacher, A., Richter, G., ...

Structural basis for the interaction of Escherichia coli NusA with protein N of phage lambda (2004)

Bonin, I., Muehlberger, R., Bourenkov, G. P., Huber, R., Bacher, A., Richter, G., ...

Caractérisation des effets des produits phytosanitaires sur les communautés d`invertébrés benthiques de la Morcille (2004)

Bacher, A.

/ Près des trois quarts du bassin versant de la Morcille sont occupés par la culture de la vigne ce qui induit une pollution diffuse du cours d`eau par ruissellement. Cette étude a pour but de...

Land uses and river ecological quality: assessment of pesticide effects on benthic invertebrates communities (2004)

Roger, M.C., Bacher, A., Gouy, V., Montuelle, B.

Rural areas could induce water pollution depending on the land uses and the agricultural practices. Vineyard are typical areas where an intensive use of pesticides cause water quality degradation :...

IspH protein of Escherichia coli: Studies on iron-sulfur cluster implementation and catalysis (2004)

Gräwert, T., Kaiser, J., Zepeck, F., Laupitz, R., Hecht, S., Amslinger, S., ...

The ispH gene of Escherichia coli specifies an enzyme catalyzing the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl diphosphate into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl...

Biochemical characterization of Bacillus subtilis type II isopentenyl diphosphate isomerase, and phylogenetic distribution of isoprenoid biosynthesis pathways (2004)

Laupitz, R., Hecht, S., Amslinger, S., Zepeck, F., Kaiser, J., Richter, G., ...

An open reading frame (Acc. no. P50740) on the Bacillus subtilis chromosome extending from bp 184 997-186 043 with similarity to the idi-2 gene of Streptomyces sp. CL190 specifying type II...

Structure of 3,4-dihydroxy-2-butanone 4-phosphate synthase from Methanococcus jannaschii in complex with divalent metal ions and the substrate ribulose 5-phosphate - Implications for the catalytic mechanism (2003)

Steinbacher,S., Schiffmann,S., Richter,G., Huber,R., Bacher,A., Fischer,M.

Optical Spectroscopy of IRAS 02091+6333 (2003)

Kimeswenger, S., Bacher, A., Emprechtinger, M., Groemer, G. E., Kapferer, W., Kausch, W., ...

We present a detailed spectroscopic investigation, spanning four winters, of the asymptotic giant branch (AGB) star IRAS 02091+6333. Zijlstra & Weinberger (2002) found a giant wall of dust around...

Structure and properties of an engineered transketolase from maize (2003)

Gerhardt,S., Echt,S., Busch,M., Freigang,J., Auerbach,G., Bader,G., ...

Crystal structure of the type II isopentenyl diphosphate: Dimethylallyl diphosphate isomerase from Bacillus subtilis (2003)

Steinbacher,S., Kaiser,J., Gerhardt,S., Eisenreich,W., Huber,R., Bacher,A., ...

Structural basis of fosmidomycin action revealed by the complex with 2-C-methyl-D-erythritol 4-phosphate synthase (IspC) - Implications for the catalytic mechanism and anti-malaria drug development (2003)

Steinbacher,S., Kaiser,J., Eisenreich,W., Huber,R., Bacher,A., Rohdich,F.

RNA DNA discrimination by the antitermination protein NusB (2003)

Mühlberger,R., Robelek,R., Eisenreich,W., Ettenhuber,C., Sinner,E. K., Kessler,H., ...

The regulation of ribosomal RNA biosynthesis in Escherichia coli by anti-termination requires binding of NusB protein to a dodecamer sequence designated boxA on the nascent RNA. The affinity of NusB...

Blue Light Perception in Plants. Detection and characterization of a light-induced neutral flavin radical in a C450A mutant of phototropin (2003)

Kay, C.W.M., Schleicher, E., Kuppig, A., Hofner, H., Rüdiger, W., Schleicher, M., ...

The LOV2 domain of Avena sativa phototropin and its C450A mutant were expressed as recombinant fusion proteins and were examined by optical spectroscopy, electron paramagnetic resonance, and...

Crystal structure of Schizosaccharomyces pombe riboflavin kinase reveals a novel ATP and riboflavin-binding fold (2003)

Bauer,S., Kemter,K., Bacher,A., Huber,R., Fischer,M., Steinbacher,S.

The essential redox cofactors riboflavin monophosphate (FMN) and flavin adenine dinucleotide (FAD) are synthesised from their precursor, riboflavin, in sequential reactions by the metal-deppndent...

Enzyme catalysis via control of activation entropy: Site-directed mutagenesis of 6,7-dimethyl-8-ribityl-lumazine synthase (2003)

Fischer,M., Haase,I., Kis,K., Meining,W., Ladenstein,R., Cushman,M., ...

Biosynthesis of pteridines. Reaction mechanism of GTP cyclohydrolase I (2003)

Rebelo,J., Auerbach,G., Bader,G., Bracher,A., Nar,H., Hösl,C., ...

GTP cyclohydrolase I catalyses the hydrolytic release of formate from GTP followed by cyclization to dihydroneopterin triphosphate. The enzymes from bacteria and animals are homodecamers containing...

Riboflavin synthase of Schizosaccharomyces pombe. Protein dynamics revealed by 19F NMR protein perturbation experiments (2003)

Fischer,M. J., Schott,A. K., Kemter,K., Feicht,R., Richter,G., Illarionov,B., ...

RNA DNA discrimination by the antitermination protein NusB (2003)

Mühlberger, R., Robelek, R., Eisenreich, W., Ettenhuber, C., Sinner, E. K., Kessler, H., ...

The regulation of ribosomal RNA biosynthesis in Escherichia coli by anti-termination requires binding of NusB protein to a dodecamer sequence designated boxA on the nascent RNA. The affinity of NusB...

Crystal structure of Schizosaccharomyces pombe riboflavin kinase reveals a novel ATP and riboflavin-binding fold (2003)

Bauer, S., Kemter, K., Bacher, A., Huber, R., Fischer, M., Steinbacher, S.

The essential redox cofactors riboflavin monophosphate (FMN) and flavin adenine dinucleotide (FAD) are synthesised from their precursor, riboflavin, in sequential reactions by the metal-deppndent...

Biosynthesis of pteridines. Reaction mechanism of GTP cyclohydrolase I (2003)

Rebelo, J., Auerbach, G., Bader, G., Bracher, A., Nar, H., Hösl, C., ...

GTP cyclohydrolase I catalyses the hydrolytic release of formate from GTP followed by cyclization to dihydroneopterin triphosphate. The enzymes from bacteria and animals are homodecamers containing...

Crystal structure of the type II isopentenyl diphosphate: Dimethylallyl diphosphate isomerase from Bacillus subtilis (2003)

Steinbacher, S., Kaiser, J., Gerhardt, S., Eisenreich, W., Huber, R., Bacher, A., ...

Enzyme catalysis via control of activation entropy: Site-directed mutagenesis of 6,7-dimethyl-8-ribityl-lumazine synthase (2003)

Fischer, M., Haase, I., Kis, K., Meining, W., Ladenstein, R., Cushman, M., ...

Structural basis of fosmidomycin action revealed by the complex with 2-C-methyl-D-erythritol 4-phosphate synthase (IspC) - Implications for the catalytic mechanism and anti-malaria drug development (2003)

Steinbacher, S., Kaiser, J., Eisenreich, W., Huber, R., Bacher, A., Rohdich, F.

Structure and properties of an engineered transketolase from maize (2003)

Gerhardt, S., Echt, S., Busch, M., Freigang, J., Auerbach, G., Bader, G., ...

Structure of 3,4-dihydroxy-2-butanone 4-phosphate synthase from Methanococcus jannaschii in complex with divalent metal ions and the substrate ribulose 5-phosphate - Implications for the catalytic mechanism (2003)

Steinbacher, S., Schiffmann, S., Richter, G., Huber, R., Bacher, A., Fischer, M.

Riboflavin synthase of Schizosaccharomyces pombe. Protein dynamics revealed by 19F NMR protein perturbation experiments (2003)

Fischer, M. J., Schott, A. K., Kemter, K., Feicht, R., Richter, G., Illarionov, B., ...

The deoxyxylulose phosphate pathway of isoprenoid biosynthesis: Studies on the mechanisms of the reactions catalyzed by IspG and IspH protein (2003)

Rohdich, F., Zepeck, F., Adam, P., Hecht, S., Kaiser, J., Laupitz, R., ...

Earlier in vivo studies have shown that the sequential action of the IspG and IspH proteins is essential for the reductive transformation of 2C-methyl-d-erythritol 2,4-cyclodiphosphate into...

Biosynthesis of riboflavin in archaea studies on the mechanism of 3,4-dihydroxy-2-butanone-4-phosphate synthase of Methanococcus jannaschii (2002)

Fischer,M., Römisch,W., Schiffmann,S., Kelly,M., Oschkinat,H., Steinbacher,S., ...

The hypothetical protein predicted by the open reading frame MJ0055 of Methanococcus jannaschii was expressed in a recombinant Escherichia coli strain under the control of a synthetic gene optimized...

Incorporation of an amide into 5-phosphonoalkyl-6-D- ribitylaminopyrimidinedione lumazine synthase inhibitors results in an unexpected reversal of selectivity for riboflavin synthase vs lumazine synthase (2002)

Cushman,M., Yang,D. L., Mihalic,J. T., Chen,J. H., Gerhardt,S., Huber,R., ...

Several analogues of a hypothetical intermediate in the reaction catalyzed by lumazine synthase were synthesized and tested as inhibitors of both Bacillus subtilis lumazine synthase and Escherichia...

Studies on the reaction mechanism of riboflavin synthase: X-ray crystal structure of a complex with 6-carboxyethyl-7-oxo-8- ribityllumazine (2002)

Gerhardt,S., Schott,A. K., Kairies,N., Cushman,M., Illarionov,B., Eisenreich,W., ...

Riboflavin synthase catalyzes the disproportionation of 6,7- dimethyl-8-ribityllumazine affording riboflavin and 5-amino-6- ribitylamino-2,4(1H,3H)-pyrimidinedione. We have determined the structure...

Formation of metal nanoclusters on specific surface sites of protein molecules (2002)

Braun,N., Meining,W., Hars,U., Fischer,M., Ladenstein,R., Huber,R., ...

During vacuum condensation of metals on frozen proteins, nanoclusters are preferentially formed at specific surface sites (decoration). Understanding the nature of metal/protein interaction is of...

Design, synthesis, and evaluation of 6-carboxyalkyl and 6- phosphonoxyalkyl derivatives of 7-oxo-8-ribitylaminolumazines as inhibitors of riboflavin synthase and lumazine synthase (2002)

Cushman,M., Yang,D. L., Gerhardt,S., Huber,R., Fischer,M., Kis,K., ...

A series of 6-carboxyalkyl and 6-phosphonoxyalkyl derivatives of 7-oxo-8-D-ribityllumazine were synthesized as inhibitors of both Escherichia coli riboflavin synthase and Bacillus subtilis lumazine...

Transcriptional regulation by antitermination. Interaction of RNA with NusB protein and NusB/NusE protein complex of Escherichia coli (2002)

Lüttgen,H., Robelek,R., Mühlberger,R., Diercks,T., Schuster,S. C., Kohler,P., ...

A recombinant heterodimeric NusB/NusE protein complex of Escherichia coli was expressed under the control of a synthetic mini operon. Surface plasmon resonance measurements showed that the...

Reaction mechanism of GTP cyclohydrolase I: Single turnover experiments using a kinetically competent reaction intermediate (2002)

Schramek,N., Bracher,A., Fischer,M., Auerbach,G., Nar,H., Huber,R., ...

Elsevier Science Ltd.GTP cyclohydrolase I catalyses the transformation of GTP into dihydroneopterin 3'-triphosphate, which is the first committed precursor of tetrahydrofolate and...

Structure of 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase involved in mevalonate-independent biosynthesis of isoprenoids (2002)

Steinbacher,S., Kaiser,J., Wungsintaweekul,J., Hecht,S., Eisenreich,W., Gerhardt,S., ...

Isoprenoids are biosynthesized from isopentenyl diphosphate and the isomeric dimethylallyl diphosphate via the mevalonate pathway or a mevalonate-independent pathway that was identified during the...

Biosynthesis of riboflavin - 6,7-dimethyl-8-ribityllumazine synthase of Schizosaccharomyces pombe (2002)

Fischer,M., Haase,I., Feicht,R., Richter,G., Gerhardt,S., Changeux,J. P., ...

A cDNA sequence from Schizosaccharomyces pombe with similarity to 6,7-dimethyl-8-ribityllumazine synthase was expressed in a recombinant Escherichia coli strain. The recombinant protein is a...

Efficient energy transfer in organic thin films - implications for organic lasers (2002)

Sheridan, A.K., Buckley, A.R., Fox, M.A., Bacher, A., Bradley, D.D.C., Samuel, I.D.W.

We show that efficient nonradiative (Förster) energy transfer between solid films of two highly luminescent perylene dyes blended in a solid film can be used to control the amplified spontaneous...

The structural basis of riboflavion binding to Schizosaccharomyces pombe 6,7-dimethyl-8-ribityllumazine synthase (2002)

Gerhardt,S., Haase,I., Steinbacher,S., Kaiser,J. T., Cushman,M., Bacher,A., ...

Biosynthesis of riboflavin - 6,7-dimethyl-8-ribityllumazine synthase of Schizosaccharomyces pombe (2002)

Fischer, M., Haase, I., Feicht, R., Richter, G., Gerhardt, S., Changeux, J. P., ...

A cDNA sequence from Schizosaccharomyces pombe with similarity to 6,7-dimethyl-8-ribityllumazine synthase was expressed in a recombinant Escherichia coli strain. The recombinant protein is a...

Structure of 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase involved in mevalonate-independent biosynthesis of isoprenoids (2002)

Steinbacher, S., Kaiser, J., Wungsintaweekul, J., Hecht, S., Eisenreich, W., Gerhardt, S., ...

Isoprenoids are biosynthesized from isopentenyl diphosphate and the isomeric dimethylallyl diphosphate via the mevalonate pathway or a mevalonate-independent pathway that was identified during the...

Reaction mechanism of GTP cyclohydrolase I: Single turnover experiments using a kinetically competent reaction intermediate (2002)

Schramek, N., Bracher, A., Fischer, M., Auerbach, G., Nar, H., Huber, R., ...

Elsevier Science Ltd.GTP cyclohydrolase I catalyses the transformation of GTP into dihydroneopterin 3'-triphosphate, which is the first committed precursor of tetrahydrofolate and...

Transcriptional regulation by antitermination. Interaction of RNA with NusB protein and NusB/NusE protein complex of Escherichia coli (2002)

Lüttgen, H., Robelek, R., Mühlberger, R., Diercks, T., Schuster, S. C., Kohler, P., ...

A recombinant heterodimeric NusB/NusE protein complex of Escherichia coli was expressed under the control of a synthetic mini operon. Surface plasmon resonance measurements showed that the...

Incorporation of an amide into 5-phosphonoalkyl-6-D- ribitylaminopyrimidinedione lumazine synthase inhibitors results in an unexpected reversal of selectivity for riboflavin synthase vs lumazine synthase (2002)

Cushman, M., Yang, D. L., Mihalic, J. T., Chen, J. H., Gerhardt, S., Huber, R., ...

Several analogues of a hypothetical intermediate in the reaction catalyzed by lumazine synthase were synthesized and tested as inhibitors of both Bacillus subtilis lumazine synthase and Escherichia...

Formation of metal nanoclusters on specific surface sites of protein molecules (2002)

Braun, N., Meining, W., Hars, U., Fischer, M., Ladenstein, R., Huber, R., ...

During vacuum condensation of metals on frozen proteins, nanoclusters are preferentially formed at specific surface sites (decoration). Understanding the nature of metal/protein interaction is of...

Design, synthesis, and evaluation of 6-carboxyalkyl and 6- phosphonoxyalkyl derivatives of 7-oxo-8-ribitylaminolumazines as inhibitors of riboflavin synthase and lumazine synthase (2002)

Cushman, M., Yang, D. L., Gerhardt, S., Huber, R., Fischer, M., Kis, K., ...

A series of 6-carboxyalkyl and 6-phosphonoxyalkyl derivatives of 7-oxo-8-D-ribityllumazine were synthesized as inhibitors of both Escherichia coli riboflavin synthase and Bacillus subtilis lumazine...

Biosynthesis of riboflavin in archaea studies on the mechanism of 3,4-dihydroxy-2-butanone-4-phosphate synthase of Methanococcus jannaschii (2002)

Fischer, M., Römisch, W., Schiffmann, S., Kelly, M., Oschkinat, H., Steinbacher, S., ...

The hypothetical protein predicted by the open reading frame MJ0055 of Methanococcus jannaschii was expressed in a recombinant Escherichia coli strain under the control of a synthetic gene optimized...

Studies on the reaction mechanism of riboflavin synthase: X-ray crystal structure of a complex with 6-carboxyethyl-7-oxo-8- ribityllumazine (2002)

Gerhardt, S., Schott, A. K., Kairies, N., Cushman, M., Illarionov, B., Eisenreich, W., ...

Riboflavin synthase catalyzes the disproportionation of 6,7- dimethyl-8-ribityllumazine affording riboflavin and 5-amino-6- ribitylamino-2,4(1H,3H)-pyrimidinedione. We have determined the structure...

The structural basis of riboflavion binding to Schizosaccharomyces pombe 6,7-dimethyl-8-ribityllumazine synthase (2002)

Gerhardt, S., Haase, I., Steinbacher, S., Kaiser, J. T., Cushman, M., Bacher, A., ...

Biosynthesis of terpenes. Preparation of (E)-1-Hydroxy-2-methyl-but-2-enyl 4-diphosphate, an intermediate of the deoxyxylulose phosphate pathway (2002)

Amslinger, S., Kis, K., Hecht, S., Adam, P., Rohdich, F., Arigoni, D., ...

(E)-1-Hydroxy-2-methyl-but-2-enyl 4-diphosphate (E-6) was synthesized in six reaction steps from hydroxyacetone (9) and (ethoxycarbonylmethenyl)-triphenylphosphorane (11) with an overall yield of...

Studies on the non-mevalonate isoprenoid biosynthetic pathway. Simple methods for preparation of isotope-labeled (E)-1-hydroxy-2-methylbut-2-enyl 4-diphosphate (2002)

Hecht, S., Amslinger, S., Jauch, J., Kis, K., Trentinaglia, V., Adam, P., ...

(E)-1-Hydroxy-2-methylbut-2-enyl 4-diphosphate (6) has been recently detected as an intermediate of isoprenoid biosynthesis via 1-deoxy-D-xylulose 5-phosphate. The compound was synthesized in two...

Studies on the nonmevalonate terpene biosynthetic pathway: Metabolic role of IspH (LytB) protein (2002)

Rohdich, F., Hecht, S., Gärtner, K., Adam, P., Krieger, C., Amslinger, S., ...

Isopentenyl diphosphate and dimethylallyl diphosphate serve as the universal precursors for the biosynthesis of terpenes. Although their biosynthesis by means of mevalonate has been studied in...

Synthesis of nanophase iron oxide in lumazine synthase capsids. (2001)

Shenton, W., Mann, S., Cölfen, H., Bacher, A., Fischer, M.

Studies on the nonmevalonate pathway to terpenes: The role of the GcpE (IspG) protein (2001)

Hecht, S., Eisenreich, W., Adam, P., Amslinger, S., Kis, K., Bacher, A., ...

Recombinant Escherichia coli cells engineered for the expression of the xyIB gene in conjunction with genes of the nonmevalonate pathway were supplied with C-13-labeled 1-deoxy-D-xylulose. Cell...

Enzyme-assisted preparation of isotope-labeled 1-deoxy-D-xylulose 5-phosphate (2001)

Hecht, S., Kis, F., Eisenreich, W., Amslinger, S., Wungsintaweekul, J., Herz, S., ...

Recombinant l-deoxy-D-xylulose 5-phosphate synthase of Bacillus subtilis was used for the preparation of isotope-labeled 1-deoxy-D-xylulose 5-phosphate using isotope-labeled glucose and/ or...

The STAR time projection chamber (1999)

Ackermann, K.H., Adams, N., Adler, C., Aluyshin, M., Ananeva, M.A., Anderson, M., ...

The STAR time projection chamber (1999)

Ackermann, K.H., Adams, N., Adler, C., Aluyshin, M., Ananeva, M.A., Anderson, M., ...

Solution structure of the antitermination protein NusB of Escherichia coli: a novel all-helical fold for an RNA-binding protein (1998)

Huenges, M., Rölz, C., Gschwind, R. M., Peteranderl, R., Berglechner, F., Richter, G., ...

The NusB protein of Escherichia coli is involved in the regulation of rRNA biosynthesis by transcriptional antitermination. In cooperation with several other proteins, it binds to a dodecamer motif...

Novel hybrid solar cells consisting of inorganic nanoparticles and an organic transport material (1997)

Hagen, J., Schaffrath, W., Otschik, P., Fink, R., Bacher, A., ...

Studies on the NusB protein of Escherichia coli; Expression and determination of the secondary structure elements by multinuclear NMR spectroscopy (1997)

Berglechner, F., Richter, G., Fischer, M., Bacher, A., Gschwind, R. M., Huenges, M., ...

The product of the nusB gene of Escherichia coli modulates the efficiency of transcription termination at I Z M ~(N utilization) sites of various bacterial and bacteriophage i genes. Similar control...

Possible Observation of the 1/2+ [880] Orbital in 249-Cm (1996)

Ahmad, I., Back, B.B., Chasman, R.R., Greene, J.P., Ishii, T., Morss, L.R., ...

This research was sponsored by the National Science Foundation Grant NSF PHY-9314783

Proton Unbound States in T=3/2 and T=2 Nuclei and the Reaction Rates for Sequential Two-proton Capture Reactions in the rp-Process (1996)

Schatz, H., Goerres, J., Herndl, H., Kaloskamis, N., Stech, E., Tischhauser, P., ...

This research was sponsored by the National Science Foundation Grant NSF PHY-9314783

Possible Observation of the 1/2+ [880] Orbital in 249-Cm (1996)

Ahmad, I., Back, B.B., Chasman, R.R., Greene, J.P., Ishii, T., Morss, L.R., ...

This research was sponsored by the National Science Foundation Grant NSF PHY-9314783

Proton Unbound States in T=3/2 and T=2 Nuclei and the Reaction Rates for Sequential Two-proton Capture Reactions in the rp-Process (1996)

Schatz, H., Goerres, J., Herndl, H., Kaloskamis, N., Stech, E., Tischhauser, P., ...

This research was sponsored by the National Science Foundation Grant NSF PHY-9314783

Search for Fragmented M1 Strength in the 48-Ca(p,p') Reaction (1995)

Mercer, D.J., Crawley, G.M., Danczyk, S., Galonsky, A., Wang, J., Bacher, A., ...

This research was sponsored by the National Science Foundation Grant NSF PHY-9314783

Quantitative evaluation of heavy metal decoration on protein molecules - contrast, specificity and resolution (1995)

Rübenkamm, E., Braun, N., Bachmann, L., Bacher, A., Brandt, J., Baumeister, W., ...

Replication by heavy metal decoration is a technique capable of portraying topochemical properties of biological surfaces. The technique has already been applied to study molecular symmetries and...

Search for Fragmented M1 Strength in the 48-Ca(p,p') Reaction (1995)

Mercer, D.J., Crawley, G.M., Danczyk, S., Galonsky, A., Wang, J., Bacher, A., ...

This research was sponsored by the National Science Foundation Grant NSF PHY-9314783

Looking for the isoscalar giant dipole resonance in 208Pd using inelastic a scattering at and near O[deg] (1994)

Davis, B. F., Akimune, H., Bacher, A., Berg, G. P. A., Fujiwara, M., Garg, U., ...

The isoscalar giant dipole resonance (ISGDR) in 208Pb has been investigated using inelastic scattering of 200 MeV [alpha] particles at and near 0[deg] where the angular distribution of the ISGDR can...

Looking for the Isoscalar Giant Dipole Resonance in Inelastic α Scattering at and Near 0˚ (1993)

Davis, B.F., Garg, U., Reviol, W., Bacher, A., Berg, G.P.A., Stephenson, E.J., ...

This research was sponsored by the National Science Foundation Grant NSF PHY-9314783

Search for High-Spin Stretched States in 206-Pb (1993)

Mihailidis, D.N., Hintz, N.M, Sethi, A., Stephenson, E.J., Bacher, A., Liu, J., ...

This research was sponsored by the National Science Foundation Grant NSF PHY-9314783

Looking for the Isoscalar Giant Dipole Resonance in Inelastic α Scattering at and Near 0˚ (1993)

Davis, B.F., Garg, U., Reviol, W., Bacher, A., Berg, G.P.A., Stephenson, E.J., ...

This research was sponsored by the National Science Foundation Grant NSF PHY-9314783

Search for High-Spin Stretched States in 206-Pb (1993)

Mihailidis, D.N., Hintz, N.M, Sethi, A., Stephenson, E.J., Bacher, A., Liu, J., ...

This research was sponsored by the National Science Foundation Grant NSF PHY-9314783

The Structure of 18-Ne and the Breakout of the Hot CNO Cycle (1992)

Hahn, K.I., Bateman, N., Magnus, P., Bacher, A., Berg, G.P.A., Stephenson, E.J.

This research was sponsored by the National Science Foundation Grant NSF PHY-9314783

High Spin States in the (p,t) Reaction (1992)

Mihailidis, D.N., Hintz, N.M., Sethi, A., Stephenson, E.J., Bacher, A., Liu, J., ...

This research was sponsored by the National Science Foundation Grant NSF PHY-9314783

Electron microscopy of decorated crystals for the determination of crystallographic rotation and translation parameters in large protein complexes (1992)

Bacher, A., Weinkauf, S., Bachmann, L., Ritsert, K., Baumeister, W., Huber, R., ...

The lumazine synthase/riboflavin synthase complex of Bacillus subtilis consists of an icosahedral capsid of 60 beta subunits enclosing a core of 3 alpha subunits. The preparation of reconstituted...

A Real-space Electron Crystallographic Method for Elucidation of Crystal Packing of a Large, Symmetric Protein Complex (1992)

Weinkauf, S., Bacher, A., Bachmann, L., Ritsert, K., Baumeister, W., Huber, R., ...

The Structure of 18-Ne and the Breakout of the Hot CNO Cycle (1992)

Hahn, K.I., Bateman, N., Magnus, P., Bacher, A., Berg, G.P.A., Stephenson, E.J.

This research was sponsored by the National Science Foundation Grant NSF PHY-9314783

High Spin States in the (p,t) Reaction (1992)

Mihailidis, D.N., Hintz, N.M., Sethi, A., Stephenson, E.J., Bacher, A., Liu, J., ...

This research was sponsored by the National Science Foundation Grant NSF PHY-9314783

K600 Small Angle Development (1991)

Berg, G.P.A., Wells, S., Wang, Y., Hall, T., Bacher, A., Bilodeau, D., ...

This research was sponsored by the National Science Foundation Grant NSF PHY-9314783

Correlation of metal decoration and topochemistry on protein surfaces (1991)

Weinkauf, S., Bacher, A., Baumeister, W., Ladenstein, R., Huber, R., Bachmann, L.

On the surface of protein molecules the formation of metal clusters during vacuum condensation is controlled by topochemical features of the substrate and by specific properties of the decorating...

K600 Small Angle Development (1991)

Berg, G.P.A., Wells, S., Wang, Y., Hall, T., Bacher, A., Bilodeau, D., ...

This research was sponsored by the National Science Foundation Grant NSF PHY-9314783

THE (p,p') SPIN TRANSFER PROGRAM AT THE INDIANA UNIVERSITY CYCLOTRON FACILITY (1990)

Opper, A., Wissink, S., Bacher, A., Lisantti, J., Olmer, C., Sawafta, R., ...

With the commissioning of a polarimeter in the focal plane of the high resolution K600 spectrometer at IUCF, we are continuing our study of spin transfer observables for discrete transitions. This...

A two-dimensional 1H NMR study on Megasphaera elsdenii flavodoxin in the reduced state. (1990)

Vervoort, J., Müller, F., Bacher, A.

13C and 15N NMR studies on the interaction between 6,7-dimethyl-8-ribityllumazine and Lumazine protein. (1990)

Vervoort, J., O'Kane, D.J., Müller, F., Bacher, A., Strobl, G., Lee, J.

K600 Small Angle Operation (1990)

Bacher, A., Berg, G.P.A., Bilodeau, D., Foster, C., Lisantti, J., Rinckel, T., ...

This research was sponsored by the National Science Foundation Grant NSF PHY-9314783

High Resolution Electron Microscopy on Crystals of the Icosahedral Lumazine Synthase/Riboflavin Synthase Complex (1990)

Weinkauf, S., Bacher, A., Schott, K., Ladenstein, R., Baumeister, W., Bachmann, L.

THE (p,p') SPIN TRANSFER PROGRAM AT THE INDIANA UNIVERSITY CYCLOTRON FACILITY (1990)

Opper, A., Wissink, S., Bacher, A., Lisantti, J., Olmer, C., Sawafta, R., ...

With the commissioning of a polarimeter in the focal plane of the high resolution K600 spectrometer at IUCF, we are continuing our study of spin transfer observables for discrete transitions. This...

THE (p,p') SPIN TRANSFER PROGRAM AT THE INDIANA UNIVERSITY CYCLOTRON FACILITY (1990)

Opper, A., Wissink, S., Bacher, A., Lisantti, J., Olmer, C., Sawafta, R., ...

With the commissioning of a polarimeter in the focal plane of the high resolution K600 spectrometer at IUCF, we are continuing our study of spin transfer observables for discrete transitions. This...

K600 Small Angle Operation (1990)

Bacher, A., Berg, G.P.A., Bilodeau, D., Foster, C., Lisantti, J., Rinckel, T., ...

This research was sponsored by the National Science Foundation Grant NSF PHY-9314783

13C, 15N and 31P-NMR studies on 6-hydroxy-L-nicotine oxidase from Arthrobacter oxidans. (1989)

Pust, S., Vervoort, J., Decker, K., Bacher, A., Müller, F.

Electron microscopy of subnanometer surface features on metal-decorated protein crystals (1989)

Bachmann, L., Weinkauf, S., Baumeister, W., Wildhaber, I., Bacher, A.

Crystals of heavy riboflavin synthase from Bacillus subtilis were freeze-etched and vacuum-coated at normal incidence with 0.1 to 0.4 nm of gold and silver, respectively. This decoration technique...

Specific Decoration Sites on Frozen-hydrated Crystals of Heavy Riboflavin Synthase (1988)

Weinkauf, S., Bacher, A., Schott, K., Wildhaber, I., Baumeister, W., Bachmann, L.

13C, 15N and 2D-NMR techniques in flavoprotein research. (1987)

Muller, F., Vervoort, J., Mayhew, S.G., Bacher, A.

13C, 15N and 31P NMR studies on 6-hydroxy-L-nicotine oxidase. (1987)

Pust, S., Vervoort, J., Decker, K., Bacher, A., Muller, F.

Enzymatic synthesis of riboflavin and FMN specifically labeled with 13C in the xylene ring. (1987)

Sedlmaier, H., Muller, F., Keller, P.J., Bacher, A.

13C, 15N and 31P NMR studies on 6-hydroxy-L-nicotine oxidase from Arthrobacter oxidans. (1987)

Pust, S., Vervoort, J., Decker, K., Bacher, A., Muller, F.

C-13, N-15 and two dimensional NMR techniques in flavoproteins research. (1987)

Muller, F., Vervoort, J., Mayhew, S.G., Bacher, A.

A comparative C13, N15 and P31 nuclear magnetic resonance study on the flavodoxins from Clostridium MP, Megasphaera elsdenii and Azotobacter vinelandii. (1986)

Vervoort, J.J.M., Muller, F., Mayhew, S.G., Moonen, C.T.W., Bacher, A.

Bacterial luciferase: A carbon-13, nitrogen-15 and phophorus-31 nuclear magnetic resonance investigation. (1986)

Vervoort, J.J.M., Muller, F., O'Kane, D.J., Lee, J., Bacher, A.

Properties of the complexes of riboflavin 3',5'-bisphosphate and the apoflavodoxins from Megasphaera elsdenii and Desulfovibrio vulgaris. (1986)

Vervoort, J.J.M., Mayhew, S.G., Muller, F., Bacher, A., Nielsen, P., ...

Heavy riboflavin synthase from bacillus subtilis. Quaternary structure and reaggregation (1986)

Bacher, A., Ben-Shaul, Y., Ludwig, H.C., Schnepple, H.

A comparative NMR study on the interaction of FMN with apoflavodoxins (1985)

Vervoort, J., Muller, F., Mayhew, S.G., Legall, J., Bacher, A.

Carbon-13 and Nitrogen-15 nuclear magnetic resonance investigations on Desulfovibrio vulgaris flavodoxin (1985)

Vervoort, J., Muller, F., Legall, J., Bacher, A., Sedlmaier, H.

Spin of deep hole states from (pd) reactions (1981)

Crawley, G.M., Kasagi, J., Gales, S., Gerlic, E., Friesel, D., Bacher, A.

Spin of deep hole states from (pd) reactions (1981)

Crawley, G.M., Kasagi, J., Gales, S., Gerlic, E., Friesel, D., Bacher, A.

Proton Elastic and Inelastic Scattering at Intermediate Energies from Isotopes of Oxygen and 9-Be as Part of a Unified Study of these Nuclei (1981)

Bertozzi, W., Buti, T., Finn, M., Hersman, F.W., Hyde, C., Kelly, J., ...

This work was supported by the National Science Foundation Grants NSF PHY 78-22774 A03, NSF PHY 81-14339, and by Indiana University

Proton Elastic and Inelastic Scattering at Intermediate Energies from Isotopes of Oxygen and 9-Be as Part of a Unified Study of these Nuclei (1981)

Bertozzi, W., Buti, T., Finn, M., Hersman, F.W., Hyde, C., Kelly, J., ...

This work was supported by the National Science Foundation Grants NSF PHY 78-22774 A03, NSF PHY 81-14339, and by Indiana University

Deep hole states observed in (p,t) reactions (1980)

Crawley, G.M., Gales, S., Weber, D., Zwieglinski, B., Benenson, W., Friesel, D., ...

Deep hole states observed in (p,t) reactions (1980)

Crawley, G.M., Gales, S., Weber, D., Zwieglinski, B., Benenson, W., Friesel, D., ...

Proton Elastic and Inelastic Scattering at Intermediate Energies from Isotopes of Oxygen and 9-Be as Part of a Unified Study of These Nuclei (1980)

Bertozzi, W., Buti, T., Finn, M., Hersman, F.W., Hyde, C., Kelly, J., ...

This work was supported by the National Science Foundation Grant NSF PHY 78-22774 A02 & A03 and by Indiana University

Proton Elastic and Inelastic Scattering at Intermediate Energies from Isotopes of Oxygen and 9-Be as Part of a Unified Study of These Nuclei (1980)

Bertozzi, W., Buti, T., Finn, M., Hersman, F.W., Hyde, C., Kelly, J., ...

This work was supported by the National Science Foundation Grant NSF PHY 78-22774 A02 & A03 and by Indiana University

Proton Elastic and Inelastic Scattering at Intermediate Energies from Isotopes of Oxygen and 9-Be as Part of a Unified Study of These Nuclei (1979)

Kelly, J., Bertozzi, W., Buti, T., Deady, M., Hersman, F.W., Hyde, C., ...

Supported by the National Science Foundation and Indiana University

Studies of Pi° Production Near Threshold (1979)

Jenkins, D., Gupta, O.P., Long, D., Bacher, A., Emery, G., Pickar, M., ...

Supported by the National Science Foundation and Indiana University

Proton Elastic and Inelastic Scattering at Intermediate Energies from Isotopes of Oxygen and 9-Be as Part of a Unified Study of These Nuclei (1979)

Kelly, J., Bertozzi, W., Buti, T., Deady, M., Hersman, F.W., Hyde, C., ...

Supported by the National Science Foundation and Indiana University

Studies of Pi° Production Near Threshold (1979)

Jenkins, D., Gupta, O.P., Long, D., Bacher, A., Emery, G., Pickar, M., ...

Supported by the National Science Foundation and Indiana University

Proton Elastic and Inelastic Scattering at Intermediate Energies from Isotopes of Oxygen and 9-Be as Part of a Unified Study of These Nuclei (1978)

Bertozzi, W., Deady, M., Hynes, M.V., Kelly, J., Norum, B., Rad, F.N., ...

This work was supported by National Science Foundation Grants PHY 76-84033A01, PHY 78-22774, and Indiana University

The 12-C, 54-Fe and 208-Pb(p,t) Reactions at E_p=80 MeV (1978)

Shepard, J.R., Anderson, R.E., Kraushaar, J.J., Ristinen, R.A., Comfort, J.R., King, N.S.P., ...

This work was supported by National Science Foundation Grants PHY 76-84033A01, PHY 78-22774, and Indiana University

Proton Elastic and Inelastic Scattering at Intermediate Energies from Isotopes of Oxygen and 9-Be as Part of a Unified Study of These Nuclei (1978)

Bertozzi, W., Deady, M., Hynes, M.V., Kelly, J., Norum, B., Rad, F.N., ...

This work was supported by National Science Foundation Grants PHY 76-84033A01, PHY 78-22774, and Indiana University

The 12-C, 54-Fe and 208-Pb(p,t) Reactions at E_p=80 MeV (1978)

Shepard, J.R., Anderson, R.E., Kraushaar, J.J., Ristinen, R.A., Comfort, J.R., King, N.S.P., ...

This work was supported by National Science Foundation Grants PHY 76-84033A01, PHY 78-22774, and Indiana University

Studies on the biosynthesis of taxol: the taxane carbon skeleton is not of mevalonoid origin.

Eisenreich, W, Menhard, B, Hylands, P J, Zenk, M H, Bacher, A

A cell culture of Taxus chinensis was established to produce the diterpene 2alpha,5alpha,10beta,14beta-tetra-acetoxy4 ++ +(20),11-taxadiene (taxuyunnanine C) in 2.6% (dry weight) yield. The...

Active site topology and reaction mechanism of GTP cyclohydrolase I.

Nar, H, Huber, R, Auerbach, G, Fischer, M, Hösl, C, Ritz, H, ...

GTP cyclohydrolase I of Escherichia coli is a torus-shaped homodecamer with D5 symmetry and catalyzes a complex ring expansion reaction conducive to the formation of dihydroneopterin triphosphate...

Dihydroneopterin triphosphate epimerase of Escherichia coli: purification, genetic cloning, and expression.

Haussmann, C, Rohdich, F, Lottspeich, F, Eberhardt, S, Scheuring, J, Mackamul, S, ...

The enzyme catalyzing the epimerization at position 2' of dihydroneopterin triphosphate was purified by a factor of about 10,000 from cell extract of Escherichia coli. The cognate gene was cloned,...

Biosynthesis of riboflavin: characterization of the bifunctional deaminase-reductase of Escherichia coli and Bacillus subtilis.

Richter, G, Fischer, M, Krieger, C, Eberhardt, S, Lüttgen, H, Gerstenschläger, I, ...

The ribG gene at the 5' end of the riboflavin operon of Bacillus subtilis and a reading frame at 442 kb on the Escherichia coli chromosome (subsequently designated ribD) show similarity with...

Biosynthesis of riboflavin: an unusual riboflavin synthase of Methanobacterium thermoautotrophicum.

Eberhardt, S, Korn, S, Lottspeich, F, Bacher, A

Riboflavin synthase was purified by a factor of about 1,500 from cell extract of Methanobacterium thermoautotrophicum. The enzyme had a specific activity of about 2,700 nmol mg(-1) h(-1) at 65...

Location of the gene coding for GTP cyclohydrolase I on the physical map of Escherichia coli.

Ritz, H, Keller, G, Richter, G, Katzenmeier, G, Bacher, A

Biosynthesis of riboflavin: cloning, sequencing, mapping, and expression of the gene coding for GTP cyclohydrolase II in Escherichia coli.

Richter, G, Ritz, H, Katzenmeier, G, Volk, R, Kohnle, A, Lottspeich, F, ...

GTP cyclohydrolase II catalyzes the first committed step in the biosynthesis of riboflavin. The gene coding for this enzyme in Escherichia coli has been cloned by marker rescue. Sequencing indicated...

Biosynthetic precursors of deazaflavins.

Reuke, B, Korn, S, Eisenreich, W, Bacher, A

The incorporation of 13C- and 14C-labeled precursors into 5-deaza-7,8-didemethyl-8-hydroxyriboflavin (factor F0) was studied with growing cells of Methanobacterium thermoautotrophicum....

Biosynthesis of riboflavin: cloning, sequencing, and expression of the gene coding for 3,4-dihydroxy-2-butanone 4-phosphate synthase of Escherichia coli.

Richter, G, Volk, R, Krieger, C, Lahm, H W, Röthlisberger, U, Bacher, A

3,4-Dihydroxy-2-butanone 4-phosphate is biosynthesized from ribulose 5-phosphate and serves as the biosynthetic precursor for the xylene ring of riboflavin. The gene coding for...

Biosynthesis of riboflavin in Bacillus subtilis: origin of the four-carbon moiety.

Le Van, Q, Keller, P J, Bown, D H, Floss, H G, Bacher, A

We studied the incorporation of [1-13C]ribose and [1,3-13C2]glycerol into the riboflavin precursor 6,7-dimethyl-8-ribityllumazine, using a riboflavin-deficient mutant of Bacillus subtilis. The...

Biosynthesis of riboflavin in Bacillus subtilis: function and genetic control of the riboflavin synthase complex.

Bacher, A, Mailänder, B

Two riboflavin synthase activities (heavy and light) have been observed in earlier studies with Bacillus subtilis. The heavy enzyme is a complex of one molecule of light enzyme (consisting of three...

Biosynthesis of Riboflavine in Saccharomyces cerevisiae: the Role of Genes rib1 and rib7

Oltmanns, O., Bacher, A.

Haploid strains of Saccharomyces cerevisiae with mutations in two different rib genes were constructed. These strains were studied by tetrad analysis and by quantitative determination of accumulation...

Human GTP cyclohydrolase I: only one out of three cDNA isoforms gives rise to the active enzyme.

Gütlich, M, Jaeger, E, Rücknagel, K P, Werner, T, Rödl, W, Ziegler, I, ...

GTP cyclohydrolase I catalyses the first and rate-limiting step of tetrahydrobiopterin biosynthesis. Its expression is regulated by interferon-gamma or kit ligand in a tissue-specific manner. Three...

The 1.25 A crystal structure of sepiapterin reductase reveals its binding mode to pterins and brain neurotransmitters.

Auerbach, G, Herrmann, A, Gütlich, M, Fischer, M, Jacob, U, Bacher, A, ...

Sepiapterin reductase catalyses the last steps in the biosynthesis of tetrahydrobiopterin, the essential co-factor of aromatic amino acid hydroxylases and nitric oxide synthases. We have determined...

Solution structure of the antitermination protein NusB of Escherichia coli: a novel all-helical fold for an RNA-binding protein.

Huenges, M, Rölz, C, Gschwind, R, Peteranderl, R, Berglechner, F, Richter, G, ...

The NusB protein of Escherichia coli is involved in the regulation of rRNA biosynthesis by transcriptional antitermination. In cooperation with several other proteins, it binds to a dodecamer motif...

Molecular cloning of a cDNA coding for GTP cyclohydrolase I from Dictyostelium discoideum.

Witter, K, Cahill, D J, Werner, T, Ziegler, I, Rödl, W, Bacher, A, ...

The GTP cyclohydrolase I (GTP-CH) gene of the cellular slime mould Dictyostelium discoideum has been cloned and sequenced. The 855 bp cDNA of this gene contains the open reading frame (ORF) encoding...

Substrate binding to DNA photolyase studied by electron paramagnetic resonance spectroscopy.

Weber, S, Richter, G, Schleicher, E, Bacher, A, Möbius, K, Kay, C W

Structural changes in Escherichia coli DNA photolyase induced by binding of a (cis,syn)-cyclobutane pyrimidine dimer (CPD) are studied by continuous-wave electron paramagnetic resonance and...

Retrobiosynthetic Nuclear Magnetic Resonance Analysis of Amino Acid Biosynthesis and Intermediary Metabolism. Metabolic Flux in Developing Maize Kernels

Glawischnig, E., Gierl, A., Tomas, A., Bacher, A., Eisenreich, W.

Studies on the biosynthesis of taxol: the taxane carbon skeleton is not of mevalonoid origin.

Eisenreich, W, Menhard, B, Hylands, P J, Zenk, M H, Bacher, A

A cell culture of Taxus chinensis was established to produce the diterpene 2alpha,5alpha,10beta,14beta-tetra-acetoxy4 ++ +(20),11-taxadiene (taxuyunnanine C) in 2.6% (dry weight) yield. The...

Active site topology and reaction mechanism of GTP cyclohydrolase I.

Nar, H, Huber, R, Auerbach, G, Fischer, M, Hösl, C, Ritz, H, ...

GTP cyclohydrolase I of Escherichia coli is a torus-shaped homodecamer with D5 symmetry and catalyzes a complex ring expansion reaction conducive to the formation of dihydroneopterin triphosphate...

Dihydroneopterin triphosphate epimerase of Escherichia coli: purification, genetic cloning, and expression.

Haussmann, C, Rohdich, F, Lottspeich, F, Eberhardt, S, Scheuring, J, Mackamul, S, ...

The enzyme catalyzing the epimerization at position 2' of dihydroneopterin triphosphate was purified by a factor of about 10,000 from cell extract of Escherichia coli. The cognate gene was cloned,...

Biosynthesis of riboflavin: characterization of the bifunctional deaminase-reductase of Escherichia coli and Bacillus subtilis.

Richter, G, Fischer, M, Krieger, C, Eberhardt, S, Lüttgen, H, Gerstenschläger, I, ...

The ribG gene at the 5' end of the riboflavin operon of Bacillus subtilis and a reading frame at 442 kb on the Escherichia coli chromosome (subsequently designated ribD) show similarity with...

Biosynthesis of riboflavin: an unusual riboflavin synthase of Methanobacterium thermoautotrophicum.

Eberhardt, S, Korn, S, Lottspeich, F, Bacher, A

Riboflavin synthase was purified by a factor of about 1,500 from cell extract of Methanobacterium thermoautotrophicum. The enzyme had a specific activity of about 2,700 nmol mg(-1) h(-1) at 65...

Location of the gene coding for GTP cyclohydrolase I on the physical map of Escherichia coli.

Ritz, H, Keller, G, Richter, G, Katzenmeier, G, Bacher, A

Biosynthesis of riboflavin: cloning, sequencing, mapping, and expression of the gene coding for GTP cyclohydrolase II in Escherichia coli.

Richter, G, Ritz, H, Katzenmeier, G, Volk, R, Kohnle, A, Lottspeich, F, ...

GTP cyclohydrolase II catalyzes the first committed step in the biosynthesis of riboflavin. The gene coding for this enzyme in Escherichia coli has been cloned by marker rescue. Sequencing indicated...

Biosynthetic precursors of deazaflavins.

Reuke, B, Korn, S, Eisenreich, W, Bacher, A

The incorporation of 13C- and 14C-labeled precursors into 5-deaza-7,8-didemethyl-8-hydroxyriboflavin (factor F0) was studied with growing cells of Methanobacterium thermoautotrophicum....

Biosynthesis of riboflavin: cloning, sequencing, and expression of the gene coding for 3,4-dihydroxy-2-butanone 4-phosphate synthase of Escherichia coli.

Richter, G, Volk, R, Krieger, C, Lahm, H W, Röthlisberger, U, Bacher, A

3,4-Dihydroxy-2-butanone 4-phosphate is biosynthesized from ribulose 5-phosphate and serves as the biosynthetic precursor for the xylene ring of riboflavin. The gene coding for...

Biosynthesis of riboflavin in Bacillus subtilis: origin of the four-carbon moiety.

Le Van, Q, Keller, P J, Bown, D H, Floss, H G, Bacher, A

We studied the incorporation of [1-13C]ribose and [1,3-13C2]glycerol into the riboflavin precursor 6,7-dimethyl-8-ribityllumazine, using a riboflavin-deficient mutant of Bacillus subtilis. The...

Biosynthesis of riboflavin in Bacillus subtilis: function and genetic control of the riboflavin synthase complex.

Bacher, A, Mailänder, B

Two riboflavin synthase activities (heavy and light) have been observed in earlier studies with Bacillus subtilis. The heavy enzyme is a complex of one molecule of light enzyme (consisting of three...

Biosynthesis of Riboflavine in Saccharomyces cerevisiae: the Role of Genes rib1 and rib7

Oltmanns, O., Bacher, A.

Haploid strains of Saccharomyces cerevisiae with mutations in two different rib genes were constructed. These strains were studied by tetrad analysis and by quantitative determination of accumulation...

Human GTP cyclohydrolase I: only one out of three cDNA isoforms gives rise to the active enzyme.

Gütlich, M, Jaeger, E, Rücknagel, K P, Werner, T, Rödl, W, Ziegler, I, ...

GTP cyclohydrolase I catalyses the first and rate-limiting step of tetrahydrobiopterin biosynthesis. Its expression is regulated by interferon-gamma or kit ligand in a tissue-specific manner. Three...

The 1.25 A crystal structure of sepiapterin reductase reveals its binding mode to pterins and brain neurotransmitters.

Auerbach, G, Herrmann, A, Gütlich, M, Fischer, M, Jacob, U, Bacher, A, ...

Sepiapterin reductase catalyses the last steps in the biosynthesis of tetrahydrobiopterin, the essential co-factor of aromatic amino acid hydroxylases and nitric oxide synthases. We have determined...

Solution structure of the antitermination protein NusB of Escherichia coli: a novel all-helical fold for an RNA-binding protein.

Huenges, M, Rölz, C, Gschwind, R, Peteranderl, R, Berglechner, F, Richter, G, ...

The NusB protein of Escherichia coli is involved in the regulation of rRNA biosynthesis by transcriptional antitermination. In cooperation with several other proteins, it binds to a dodecamer motif...

Molecular cloning of a cDNA coding for GTP cyclohydrolase I from Dictyostelium discoideum.

Witter, K, Cahill, D J, Werner, T, Ziegler, I, Rödl, W, Bacher, A, ...

The GTP cyclohydrolase I (GTP-CH) gene of the cellular slime mould Dictyostelium discoideum has been cloned and sequenced. The 855 bp cDNA of this gene contains the open reading frame (ORF) encoding...

Substrate binding to DNA photolyase studied by electron paramagnetic resonance spectroscopy.

Weber, S, Richter, G, Schleicher, E, Bacher, A, Möbius, K, Kay, C W

Structural changes in Escherichia coli DNA photolyase induced by binding of a (cis,syn)-cyclobutane pyrimidine dimer (CPD) are studied by continuous-wave electron paramagnetic resonance and...

Retrobiosynthetic Nuclear Magnetic Resonance Analysis of Amino Acid Biosynthesis and Intermediary Metabolism. Metabolic Flux in Developing Maize Kernels

Glawischnig, E., Gierl, A., Tomas, A., Bacher, A., Eisenreich, W.

Stability of uracil and thymine cations

Beikircher, M., Ptasinska, S., Feil, S., Winkler, M., Bacher, A., Schustereder, W., ...

Stability of uracil and thymine cations

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Stability of uracil and thymine cations

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Elementary plasma reactions revisited: Electron ionization and ion surface reactions relevant for fusion plasmas

Feketeova, L., Feil, S., Grill, V., Endstrasser, N., Rasul, B., Schustereder, W., ...