Far upstream sequences regulate the human prolactin promoter transcription (2000)
Van De Weerdt, Cécile, Peers, Bernard, Belayew, A, Martial, Joseph, Muller, Marc
The human prolactin gene is mainly expressed in pituitary lactotrope cells, but transcription from an alternative, far upstream promoter was detected in lymphoid, placental and mammary cells. We...
Bulens, F, Stockmans, I, De Vriese, A, Convents, R, ...
Tissue-type plasminogen activator (t-PA) is a positive modulator of the plasminogen-plasmin system, which is involved in bone remodeling. In the present study, 1,25-dihydroxyvitamin D(3)...
Characterization and targeting of the murine alpha2-antiplasmin gene (1997)
Belayew, A, Matsuo, O, ...
Alpha2-Antiplasmin (alpha2-AP) is the main physiological plasmin inhibitor in mammalian plasma. As a first step toward the generation of alpha2-AP deficient mice, the murine alpha2-AP gene was...
Jacquemin, P, Alsat, E, Oury, Cécile, Belayew, A, Muller, Marc, Evain-Brion, D, ...
The hCS-A and hCS-B genes encoding human chorionic somatomammotropin and the related hCS-L gene are very similar in their coding and flanking sequences. For each of these genes, downstream enhancers,...
Poncelet, AC, Levavi-Sivan, B, Muller, Marc, Yaron, Z, Martial, Joseph, Belayew, A
To study the elements involved in the pituitary specific transcriptional regulation of the tilapia prolactin I gene (tiPRL I), we have cloned and entirely sequenced a 3.4-kb genomic fragment...
Volckaert, F, Poncelet, AC, Hellemans, Bart; U0001912, Ollevier, Frans; U0015657, Martial, JA, Belayew, A
Wera, S, Zheng, L, Hooghe-Peters, E L, Belayew, A, Martial, Joseph, Velkeniers, B
It is at present well established that prolactin exerts a non-specific immunoactivating function. In this work we tested whether the immunosuppressant drugs cyclosporin A, FK506 and rapamycin...
Hellemans, B A, Galbusera, P, Sekkali, B, Belayew, A
The transfer of exogenous DNA in fish represents a powerful strategy to study the regulation of gene expression in vivo. The African catfish (Clarias gariepinus) was chosen for this study because of...
Jacquemin, P, Oury, Cécile, Belayew, A, Martial, Joseph
The transcriptional activity of the human placental lactogen genes (choriosomatomammotropic hormone, hCS) is controlled by tissue-specific enhancers located 4 kb downstream from their respective...
A comparative study of reporter gene activities in fish cells and embryos (1994)
Sekkali, B, Hellemans, B, Belayew, A, Martial, Joseph, Ollivier, F, Volckaert, FA
Efficient lipofection of human trophoblast cells in primary cultures (1993)
Jacquemin, P, Alsat, E, Oury, Cécile, Belayew, A, Martial, Joseph, Evain-Brion, D
Human choriosomatomammotropic hormone, also known as placental lactogen, is expressed in syncytiotrophoblast cells of the placenta. Studying transcriptional regulation of the genes coding for this...
Wera, S, Belayew, A, Martial, Joseph
Human PRL (hPRL) gene expression is controlled by cAMP and Ca2+. This control is mediated by two cis-elements: a Pit-1 binding site (-62 to -35) and sequence A (-110 to -85), present in the hPRL...
Molecular Biology of tilapia prolactins (1993)
Swennen, E, Sekkali, B, Poncelet, AC, Rentier-Delrue, Françoise, Martial, Joseph, Belayew, A
Argenton, F, Vianello, S, Bernardini, S, Jacquemin, P, Martial, Joseph, Belayew, A, ...
Growth hormone (GH) gene expression in mammals is regulated by the interaction of the transcription factor Pit-1 with two binding sites within the proximal promoter. Four sequences, homologous to the...
Structure of the tilapia (Oreochromis mossambicus) prolactin I gene (1992)
Swennen, D, Poncelet, AC, Sekkali, B, Rentier-Delrue, Françoise, Martial, Joseph, Belayew, A
The tilapia (Oreochromis mossambicus) prolactin-I (PRL-I) gene has been cloned and sequenced. Its transcript (3,677 bases long) begins with a guanine and is organized in five exons and four introns...
Berwaer, M, Monget, P, Peers, Bernard, Mathy, Marianne, Bellefroid, E, Davis, J R, ...
We have cloned DNA sequences extending up to 6000 bp upstream from the first exon of the human prolactin (hPRL) gene. 5000 bp of these upstream sequences were fused to a CAT reporter gene and shown...
Triiodothyronine inhibits transcription from the human growth hormone promoter (1990)
Morin, A, Louette, J, Voz, Marianne, Tixier-Vidal, A, Belayew, A, Martial, Joseph
Three DNA constructs, the natural human growth hormone gene (hGH-hGH) its 500 bp promoter linked to the chloramphenicol acetyl transferase reporter gene (hGH-CAT), and its structural part linked to...
The human genome contains hundreds of genes coding for finger proteins of the Kruppel type (1989)
Bellefroid, E J, Lecocq, P, Benhida, A, Poncelet, D, Belayew, A, Martial, Joseph
Our aim was to identify new human proteins with potential DNA binding activity, related to the Kruppel protein which regulates Drosophila segmentation. We screened a human placenta cDNA library and a...
Lemaigre, F P, Peers, Bernard, Lafontaine, D.A., Mathy, Marianne, Rousseau, G G, Belayew, A, ...
The human genes coding for growth hormone (GH), chorionic somatomammotropin (placental lactogen, CS), and prolactin (Prl) are related evolutionarily but are expressed in phenotypically distinct cell...
Hooghe-Peters, E L, Belayew, A, Herregodts, P, Velkeniers, B, Smets, G, Martial, Joseph, ...
Data are controversial concerning the time when PRL-synthesizing cells are detected for the first time in the rat pituitary. Using a very sensitive immunocytochemical technique, we could visualize...
Rajpert, E J, Lemaigre, F P, Eliard, P H, Place, M, Lafontaine, DA, Economidis, I V, ...
Modulation of gene expression by glucocorticoids involves interaction of these hormones with an intracellular receptor followed by 'transformation' of the hormone-receptor complex into a nuclear...
Belayew, A, Bellefroid, E, Berwaer, M, Dumoulin, M, Lambert, C, Lemaitre-Wathy, C, ...
We are studying nucleotide sequences responsible for the regulation of eukaryotic gene expression. Our test system comprises the human genes coding for prolactin (hPRL), growth hormone (hGH-N) and...
Location of transcriptional and regulatory sequences within the prolactin family genes (1986)
Belayew, A, Bellefroid, E, Berwaer, M, Dumoulin, M, Lambert, C, Lemaitre-Wathy, C, ...
Vertebrate cdnas to peptide-hormones hybridize to insect rna dot blots (1985)
Cardoen, J, Belayew, A, Lecomte, C, ...
Eliard, P H, Marchand, M, Rousseau, G G, Formstecher, P, Mathy, Marianne, Belayew, A, ...
An in vitro competition assay was used to investigate whether binding sites for the human glucocorticoid receptor occur in the human genes for growth hormone (hGH) and placental lactogen (chorionic...
Regulatory sequences in the human prolactin gene (1984)
Martial, Joseph, Truong, A T, Eliard, P, Mathy, Marianne, Rousseau, G G, Belayew, A
Endocrinology: Proceedings of the 7th International Congress of Endocrinology
Transcriptional control of the murine albumin/alpha-fetoprotein locus during development.
The ontogeny of expression of the alpha-fetoprotein (AFP) and albumin genes was examined in livers from late prenatal to 1-month-postpartum C3H/He mice. A parallel accumulation of both AFP and...
Jacquemin, P, Oury, C, Peers, B, Morin, A, Belayew, A, Martial, J A
The human genes coding for growth hormone (hGH) and placental lactogen (choriosomatomammotropic hormone [hCS]) are clustered on chromosome 17 in the following order: 5' hGH-N hCS-L hCS-A hGH-V hCS-B...
Voz, M L, Peers, B, Wiedig, M J, Jacquemin, P, Belayew, A, Martial, J A
Human placental lactogen B (hCS-B) promoter activity is strongly stimulated by triiodothyronine (T3) in pituitary GC cells through interaction between the thyroid receptor and a thyroid...
Regulatory elements controlling pituitary-specific expression of the human prolactin gene.
Peers, B, Voz, M L, Monget, P, Mathy-Hartert, M, Berwaer, M, Belayew, A, ...
We have performed transfection and DNase I footprinting experiments to investigate pituitary-specific expression of the human prolactin (hPRL) gene. When fused to the chloramphenicol...
Genetic analysis of alpha-fetoprotein synthesis in mice.
The differential induction of alpha-fetoprotein (AFP) mRNA during liver regeneration in three inbred strains of mice was examined to determine the genetic and molecular bases for the differences in...
Barlow, J W, Voz, M L, Eliard, P H, Mathy-Harter, M, De Nayer, P, Economidis, I V, ...
The intracellular receptor for thyroid hormone is a protein found in chromatin. Since thyroid hormone stimulates transcription of the growth hormone gene through an unknown mechanism, the hypothesis...
Locus unlinked to alpha-fetoprotein under the control of the murine raf and Rif genes.
Pachnis, V, Belayew, A, Tilghman, S M
The levels of alpha-fetoprotein mRNA in mice are determined by at least two trans-acting, unlinked genes, raf and Rif. raf determines the basal levels of alpha-fetoprotein mRNA in adult mice, while...
Isolation and characterization of the human prolactin gene.
Truong, A T, Duez, C, Belayew, A, Renard, A, Pictet, R, Bell, G I, ...
Prolactin (PRL) and growth hormone (GH) genes derive from a common ancestor and still share some sequence homologies. Their expression in the pituitary gland is regulated in opposite directions by...
Transcriptional control of the murine albumin/alpha-fetoprotein locus during development.
The ontogeny of expression of the alpha-fetoprotein (AFP) and albumin genes was examined in livers from late prenatal to 1-month-postpartum C3H/He mice. A parallel accumulation of both AFP and...
Jacquemin, P, Oury, C, Peers, B, Morin, A, Belayew, A, Martial, J A
The human genes coding for growth hormone (hGH) and placental lactogen (choriosomatomammotropic hormone [hCS]) are clustered on chromosome 17 in the following order: 5' hGH-N hCS-L hCS-A hGH-V hCS-B...
Voz, M L, Peers, B, Wiedig, M J, Jacquemin, P, Belayew, A, Martial, J A
Human placental lactogen B (hCS-B) promoter activity is strongly stimulated by triiodothyronine (T3) in pituitary GC cells through interaction between the thyroid receptor and a thyroid...
Regulatory elements controlling pituitary-specific expression of the human prolactin gene.
Peers, B, Voz, M L, Monget, P, Mathy-Hartert, M, Berwaer, M, Belayew, A, ...
We have performed transfection and DNase I footprinting experiments to investigate pituitary-specific expression of the human prolactin (hPRL) gene. When fused to the chloramphenicol...
Genetic analysis of alpha-fetoprotein synthesis in mice.
The differential induction of alpha-fetoprotein (AFP) mRNA during liver regeneration in three inbred strains of mice was examined to determine the genetic and molecular bases for the differences in...
Barlow, J W, Voz, M L, Eliard, P H, Mathy-Harter, M, De Nayer, P, Economidis, I V, ...
The intracellular receptor for thyroid hormone is a protein found in chromatin. Since thyroid hormone stimulates transcription of the growth hormone gene through an unknown mechanism, the hypothesis...
Locus unlinked to alpha-fetoprotein under the control of the murine raf and Rif genes.
Pachnis, V, Belayew, A, Tilghman, S M
The levels of alpha-fetoprotein mRNA in mice are determined by at least two trans-acting, unlinked genes, raf and Rif. raf determines the basal levels of alpha-fetoprotein mRNA in adult mice, while...
Isolation and characterization of the human prolactin gene.
Truong, A T, Duez, C, Belayew, A, Renard, A, Pictet, R, Bell, G I, ...
Prolactin (PRL) and growth hormone (GH) genes derive from a common ancestor and still share some sequence homologies. Their expression in the pituitary gland is regulated in opposite directions by...