Physical mapping of the minimal region of loss in 5q- chromosome.
Fairman, J, Chumakov, I, Chinault, A C, Nowell, P C, Nagarajan, L
Acquired interstitial loss of all or part of the long arm of human chromosome 5 (5q-) is an anomaly that is seen frequently in patients with preleukemic myelodysplasia and acute myelogenous leukemia....
We have used fluorescence in situ hybridization on interphase nuclei of normal female cells to compare the replication timing patterns of genes on the human X chromosome that are known to escape X...
Nelson, D L, Ballabio, A, Victoria, M F, Pieretti, M, Bies, R D, Gibbs, R A, ...
Over 400 yeast artificial chromosome (YAC) clones were isolated from the human X chromosome, and 110 of these were assigned to regions defined by chromosome translocation and deletion breakpoints....
Jayakumar, A, Hwang, S J, Fabiny, J M, Chinault, A C, Barnes, E M
During nitrogen-limited growth, Escherichia coli expresses a specific ammonium or methylammonium ion transport system (Amt). Strains carrying defects in Amt have been isolated following Tn10...
Analysis of mobilization elements in plasmids from Shigella flexneri.
Roessler, E, Fenwick, R G, Chinault, A C
The mobilization properties of three plasmids were examined after cotransfer from Shigella flexneri to Escherichia coli. The largest plasmid, pCN1, was shown to be a conjugative R factor that could...
Konecki, D S, Brennand, J, Fuscoe, J C, Caskey, C T, Chinault, A C
Recombinant plasmids containing DNA inserts complementary to mRNA coding for hypoxanthine-guanine phosphoribosyltransferase (HPRT) from mouse and Chinese hamster cell lines have been isolated from...
Rapid identification of yeast artificial chromosome clones by matrix pooling and crude lysate PCR.
Kwiatkowski, T J, Zoghbi, H Y, Ledbetter, S A, Ellison, K A, Chinault, A C
Yen, P H, Patel, P, Chinault, A C, Mohandas, T, Shapiro, L J
Previous theoretical considerations and some experimental data have suggested a role for DNA methylation in the maintenance of mammalian X chromosome inactivation. The isolation of specific X-encoded...
Brennand, J, Chinault, A C, Konecki, D S, Melton, D W, Caskey, C T
Cloned cDNA sequences of the murine hypoxanthine/guanine phosphoribosyltransferase (HPRT; EC 2.4.2.8) gene have been isolated by using a mouse neuroblastoma cell line containing increased levels of a...
Virta-Pearlman, V J, Gunaratne, P H, Chinault, A C
A 4-kb HindIII fragment that supported the efficient autonomous replication of plasmid vector pDY-, a replication-defective construct based on Epstein-Barr virus sequences, in human K562 cells was...
Fine structure of the human hypoxanthine phosphoribosyltransferase gene.
Patel, P I, Framson, P E, Caskey, C T, Chinault, A C
The human hypoxanthine phosphoribosyltransferase (HPRT) gene has been characterized by molecular cloning, mapping, and DNA sequencing techniques. The entire gene, which is about 44 kilobases in...
Isomeric aminoacyl-tRNAs are both bound by elongation factor Tu.
Hecht, S M, Tan, K H, Chinault, A C, Arcari, P
Recent suggestions that elongation factor Tu (EF-Tu) is specific for 2'-O-aminoacyl-tRNA, as compared with the 3'-isomer, prompted us to assay [3H]aminoacyl-tRNAs from Escherichia coli terminating in...
Physical mapping of the minimal region of loss in 5q- chromosome.
Fairman, J, Chumakov, I, Chinault, A C, Nowell, P C, Nagarajan, L
Acquired interstitial loss of all or part of the long arm of human chromosome 5 (5q-) is an anomaly that is seen frequently in patients with preleukemic myelodysplasia and acute myelogenous leukemia....
We have used fluorescence in situ hybridization on interphase nuclei of normal female cells to compare the replication timing patterns of genes on the human X chromosome that are known to escape X...
Nelson, D L, Ballabio, A, Victoria, M F, Pieretti, M, Bies, R D, Gibbs, R A, ...
Over 400 yeast artificial chromosome (YAC) clones were isolated from the human X chromosome, and 110 of these were assigned to regions defined by chromosome translocation and deletion breakpoints....
Jayakumar, A, Hwang, S J, Fabiny, J M, Chinault, A C, Barnes, E M
During nitrogen-limited growth, Escherichia coli expresses a specific ammonium or methylammonium ion transport system (Amt). Strains carrying defects in Amt have been isolated following Tn10...
Analysis of mobilization elements in plasmids from Shigella flexneri.
Roessler, E, Fenwick, R G, Chinault, A C
The mobilization properties of three plasmids were examined after cotransfer from Shigella flexneri to Escherichia coli. The largest plasmid, pCN1, was shown to be a conjugative R factor that could...
Konecki, D S, Brennand, J, Fuscoe, J C, Caskey, C T, Chinault, A C
Recombinant plasmids containing DNA inserts complementary to mRNA coding for hypoxanthine-guanine phosphoribosyltransferase (HPRT) from mouse and Chinese hamster cell lines have been isolated from...
Rapid identification of yeast artificial chromosome clones by matrix pooling and crude lysate PCR.
Kwiatkowski, T J, Zoghbi, H Y, Ledbetter, S A, Ellison, K A, Chinault, A C
Yen, P H, Patel, P, Chinault, A C, Mohandas, T, Shapiro, L J
Previous theoretical considerations and some experimental data have suggested a role for DNA methylation in the maintenance of mammalian X chromosome inactivation. The isolation of specific X-encoded...
Brennand, J, Chinault, A C, Konecki, D S, Melton, D W, Caskey, C T
Cloned cDNA sequences of the murine hypoxanthine/guanine phosphoribosyltransferase (HPRT; EC 2.4.2.8) gene have been isolated by using a mouse neuroblastoma cell line containing increased levels of a...
Virta-Pearlman, V J, Gunaratne, P H, Chinault, A C
A 4-kb HindIII fragment that supported the efficient autonomous replication of plasmid vector pDY-, a replication-defective construct based on Epstein-Barr virus sequences, in human K562 cells was...
Fine structure of the human hypoxanthine phosphoribosyltransferase gene.
Patel, P I, Framson, P E, Caskey, C T, Chinault, A C
The human hypoxanthine phosphoribosyltransferase (HPRT) gene has been characterized by molecular cloning, mapping, and DNA sequencing techniques. The entire gene, which is about 44 kilobases in...
Isomeric aminoacyl-tRNAs are both bound by elongation factor Tu.
Hecht, S M, Tan, K H, Chinault, A C, Arcari, P
Recent suggestions that elongation factor Tu (EF-Tu) is specific for 2'-O-aminoacyl-tRNA, as compared with the 3'-isomer, prompted us to assay [3H]aminoacyl-tRNAs from Escherichia coli terminating in...
A YAC contig encompassing the recessive Stargardt disease gene (STGD) on chromosome 1p.
Anderson, K L, Baird, L, Lewis, R A, Chinault, A C, Otterud, B, Leppert, M, ...
Stargardt disease (STGD) and fundus flavimaculatus are infrequent autosomal recessive conditions characterized by a juvenile macular dystrophy and variable degrees of peripheral retinal changes....
Subramanian, P. S., Nelson, D. L., Chinault, A. C.
Trinucleotide repeat expansions have been implicated in the causation of a number of neurodegenerative disorders. In the case of fragile X syndrome, full expansion of the FMR1 repeat element (CGG)n...