Structural insights into the catalytic mechanism of trypanosoma cruzi trans -sialidase (2004)
Amaya, M. F., Watts, A. G., Damager, I., Wehenkel, A., Nguyen, T., Buschiazzo, A., ...
Aguero, F., Campo, V., Cremona, L., Jager, A., Di Noia, J. M., Overath, P., ...
A total of 1,921 expressed sequence tags (ESTs) were obtained from bloodstream trypomastigotes of Trypanosoma carassii, a parasite of economic importance due to its high prevalence in fish farms....
Fetal IgG specificities against Trypanosoma cruzi antigens in infected newborns.
Reyes, M B, Lorca, M, Muñoz, P, Frasch, A C
A panel of Trypanosoma cruzi antigens produced by recombinant DNA techniques was used to analyze the IgM and IgG specificities present in sera from 22 mothers with chronic Chagas disease and their...
Shift of excretory-secretory immunogens of Trypanosoma cruzi during human Chagas' disease.
Jazín, E E, Luquetti, A O, Rassi, A, Frasch, A C
We studied secreted-excreted immunogens in human patients infected with Trypanosoma cruzi. A pair of 45- to 55-kDa antigens and a family of shed acute-phase antigens characterized the acute phase,...
The major cysteine proteinase (cruzipain) from Trypanosoma cruzi is antigenic in human infections.
Martinez, J, Campetella, O, Frasch, A C, Cazzulo, J J
Antibodies against the major cysteine proteinase (cruzipain) from Trypanosoma cruzi were detected in human sera obtained from patients with chronic Chagas' disease. Not only the intact 60-kDa enzyme...
Direct detection of Vibrio cholerae in stool samples.
Varela, P, Pollevick, G D, Rivas, M, Chinen, I, Binsztein, N, Frasch, A C, ...
A direct method to detect Vibrio cholerae in stool samples was developed by using a PCR procedure that did not require a DNA purification step. Dilution (1/100) of stool samples prevented inhibition...
Vergara, U, Lorca, M, Veloso, C, Gonzalez, A, Engstrom, A, Aslund, L, ...
Synthetic peptides modelled according to the amino acid sequences derived from the repeated domains of five Trypanosoma cruzi antigens were used in an immunoradiometric assay to detect antibodies...
Leguizamón, M S, Campetella, O E, González Cappa, S M, Frasch, A C
trans-Sialidase (TS) is an enzymatic activity described only for trypanosomes that is involved in the invasion of host cells by Trypanosoma cruzi. The enzyme that is shed by the parasite is made of...
De Lange, T, Berkvens, T M, Veerman, H J, Frasch, A C, Barry, J D, Borst, P
Messenger RNAs of Trypanosoma brucei share a common 5' terminal sequence of 35 nucleotides, encoded by a mini-exon located in 1.35-kb tandemly linked repeats. We show here that sequences, almost...
Identification of the gene(s) coding for the trans-sialidase of Trypanosoma cruzi.
Parodi, A J, Pollevick, G D, Mautner, M, Buschiazzo, A, Sanchez, D O, Frasch, A C
The gene(s) encoding the Trypanosoma cruzi shed-acute-phase-antigen (SAPA) has a 5' end encoding a region containing two totally and two partially conserved Ser-X-Asp-X-Gly-X-Thr-Trp motifs which are...
Two forms of 'malic' enzyme with different regulatory properties in Trypanosoma cruzi.
Cannata, J J, Frasch, A C, Cataldi De Flombaum, M A, Segura, E L, Cazzulo, J J
1. Cell-free extracts from culture epimastigotes of Trypanosoma cruzi contained two forms of NADP+-linked 'malic' enzyme (EC 1.1.1.40), I and II, with the same molecular weight but different...
Structural basis of sialyltransferase activity in trypanosomal sialidases.
Buschiazzo, A, Tavares, G A, Campetella, O, Spinelli, S, Cremona, M L, París, G, ...
The intracellular parasite Trypanosoma cruzi, the etiological agent of Chagas disease, sheds a developmentally regulated surface trans-sialidase, which is involved in key aspects of parasite-host...
Barderi, P, Campetella, O, Frasch, A C, Santomé, J A, Hellman, U, Pettersson, U, ...
NADP-linked glutamate dehydrogenase (NADP+-GluDH, EC 1.4.1.4) has been purified to homogeneity from epimastigotes of Trypanosoma cruzi by an improved procedure, and the amino acid sequences of 11...
Fetal IgG specificities against Trypanosoma cruzi antigens in infected newborns.
Reyes, M B, Lorca, M, Muñoz, P, Frasch, A C
A panel of Trypanosoma cruzi antigens produced by recombinant DNA techniques was used to analyze the IgM and IgG specificities present in sera from 22 mothers with chronic Chagas disease and their...
Shift of excretory-secretory immunogens of Trypanosoma cruzi during human Chagas' disease.
Jazín, E E, Luquetti, A O, Rassi, A, Frasch, A C
We studied secreted-excreted immunogens in human patients infected with Trypanosoma cruzi. A pair of 45- to 55-kDa antigens and a family of shed acute-phase antigens characterized the acute phase,...
The major cysteine proteinase (cruzipain) from Trypanosoma cruzi is antigenic in human infections.
Martinez, J, Campetella, O, Frasch, A C, Cazzulo, J J
Antibodies against the major cysteine proteinase (cruzipain) from Trypanosoma cruzi were detected in human sera obtained from patients with chronic Chagas' disease. Not only the intact 60-kDa enzyme...
Direct detection of Vibrio cholerae in stool samples.
Varela, P, Pollevick, G D, Rivas, M, Chinen, I, Binsztein, N, Frasch, A C, ...
A direct method to detect Vibrio cholerae in stool samples was developed by using a PCR procedure that did not require a DNA purification step. Dilution (1/100) of stool samples prevented inhibition...
Vergara, U, Lorca, M, Veloso, C, Gonzalez, A, Engstrom, A, Aslund, L, ...
Synthetic peptides modelled according to the amino acid sequences derived from the repeated domains of five Trypanosoma cruzi antigens were used in an immunoradiometric assay to detect antibodies...
Leguizamón, M S, Campetella, O E, González Cappa, S M, Frasch, A C
trans-Sialidase (TS) is an enzymatic activity described only for trypanosomes that is involved in the invasion of host cells by Trypanosoma cruzi. The enzyme that is shed by the parasite is made of...
De Lange, T, Berkvens, T M, Veerman, H J, Frasch, A C, Barry, J D, Borst, P
Messenger RNAs of Trypanosoma brucei share a common 5' terminal sequence of 35 nucleotides, encoded by a mini-exon located in 1.35-kb tandemly linked repeats. We show here that sequences, almost...
Identification of the gene(s) coding for the trans-sialidase of Trypanosoma cruzi.
Parodi, A J, Pollevick, G D, Mautner, M, Buschiazzo, A, Sanchez, D O, Frasch, A C
The gene(s) encoding the Trypanosoma cruzi shed-acute-phase-antigen (SAPA) has a 5' end encoding a region containing two totally and two partially conserved Ser-X-Asp-X-Gly-X-Thr-Trp motifs which are...
Two forms of 'malic' enzyme with different regulatory properties in Trypanosoma cruzi.
Cannata, J J, Frasch, A C, Cataldi De Flombaum, M A, Segura, E L, Cazzulo, J J
1. Cell-free extracts from culture epimastigotes of Trypanosoma cruzi contained two forms of NADP+-linked 'malic' enzyme (EC 1.1.1.40), I and II, with the same molecular weight but different...
Structural basis of sialyltransferase activity in trypanosomal sialidases.
Buschiazzo, A, Tavares, G A, Campetella, O, Spinelli, S, Cremona, M L, París, G, ...
The intracellular parasite Trypanosoma cruzi, the etiological agent of Chagas disease, sheds a developmentally regulated surface trans-sialidase, which is involved in key aspects of parasite-host...
Barderi, P, Campetella, O, Frasch, A C, Santomé, J A, Hellman, U, Pettersson, U, ...
NADP-linked glutamate dehydrogenase (NADP+-GluDH, EC 1.4.1.4) has been purified to homogeneity from epimastigotes of Trypanosoma cruzi by an improved procedure, and the amino acid sequences of 11...