A. D. L. Akkermans

Feeding of Lactobacillus sobrius reduces Escherichia coli F4 levels in the gut and promotes growth of infected piglets (still E-pub) (2008)

Konstantinov, S.R., Smidt, H., Akkermans, A.D.L., Casini, L., Trevisi, P., Mazzoni, M., ...

The microbial community in the guts of mammals is often seen as an important potential target in therapeutic and preventive interventions. The aim of the present study was to determine whether...

16S rRNA targeted DGGE fingerprinting of microbial communities (2008)

Tzeneva, V.A., Heilig, G.H.J., Akkermans, A.D.L., Smidt, H.

The past decades have seen the staggering development of molecular microbial ecology as a discipline that uses the detection of so-called biomarkers to monitor microbial communities in environment...

Lactobacillus sobrius sp. nov., a novel isolate abundant in the intestine of weaning piglets (2006)

Konstantinov, S.R., Poznanski, E., Fuentes, S., Akkermans, A.D.L., Smidt, H.

To obtain porcine isolates related to Lactobacillus amylovorus, we screened strains from piglet intestine grown on Lactobacillus-specific MRS agar for hybridization to a fluorescent 16S rRNA-targeted...

Post-natal development of the porcine microbiota composition and activities (2006)

Konstantinov, S.R., Awati, A.A., Williams, B.A., Miller, B.G., Jones, P., Stokes, C.R., ...

The current study describes the development of the porcine microbiota and its metabolic activities during the neonatal and weaning period. Using 16S rRNA-based approaches, we first analysed the ileal...

Community analysis of a full-scale anaerobic bioreactor treating paper mill wastewater (2005)

Roest, C., Heilig, G.H.J., Smidt, H., Stams, A.J.M., Akkermans, A.D.L.

The first true obligately syntrophic propionate-oxidizing bacterium, Pelotomaculum schinkii sp.nov., co-cultured with Methanospirillum hungatei, and emended description of the genus Pelotomaculum (2005)

Harmsen, H.J., Plugge, C.M., Akkermans, A.D.L., ...

A Gram-positive, spore-forming, syntrophic propionate-oxidizing bacterium, Pelotomaculum schinkii sp. nov. strain HHT, was isolated as a co-culture with Methanospirillum hungatei JF-1T from...

Effect of substrate adaptation on the microbial fermentation and microbial composition of faecal microbiota of weaning piglets studied in vitro (2005)

Awati, A.A., Konstantinov, S.R., Williams, B.A., Akkermans, A.D.L., Bosch, M.W., Smidt, H., ...

The in vitro cumulative gas production technique can be used to assess microbial activity of a complex community, in relation to fermentation of a particular energy source. Therefore, in combination...

Ecology and activity of clostridia in the intestine of mammals (2005)

Konstantinov, S.R., Smidt, H., Akkermans, A.D.L.

In vitro assessment of gastrointestinal tract (GIT) fermentation: Fermentable substrates and microbial activity (2005)

Williams, B.A., Bosch, M.W., Awati, A.A., Konstantinov, S.R., Smidt, H., Akkermans, A.D.L., ...

Recently, it has become apparent that GIT fermentation is not only of interest for ruminant animals, but also for monogastrics. While it is now widely accepted that the fermentation process and its...

Lactobacillus sobrius sp.nov., a novel isolate abundant in the intestine of weaning piglets (2005)

Konstantinov, S.R., Poznanski, E., Fuentes, S., Akkermans, A.D.L., Smidt, H.

Community analysis of a full-scale anaerobic bioreactor treating paper mill wastwater (2005)

Roest, C., Heilig, G.H.J., Smidt, H., Stams, A.J.M., Akkermans, A.D.L.

To get insight into the microbial community of an Upflow Anaerobic Sludge Blanket reactor treating paper mill wastewater, conventional microbiological methods were combined with 16S rRNA gene...

Enrichment and detection of microorganisms involved in direct and indirect methanogenesis from methanol in an anaerobic thermophilic bioreactor (2005)

Altinbas, M., Paulo, P.L., Akkermans, A.D.L., Smidt, H., ...

To gain insight into the microorganisms involved in direct and indirect methane formation from methanol in a laboratory-scale thermophilic (55°C) methanogenic bioreactor, reactor sludge was...

Microbial diversity studies of the porcine gastrointestinal ecosystem during weaning transition (2004)

Konstantinov, S.R., Favier, C.F., Williams, B.A., Kluss, J., ...

Postnatal development of intestinal immune system in piglets: implications for the process of weaning (2004)

Stokes, C.R., Bailey, M., Haverson, K., Harris, C., Jones, P., Inman, C., ...

Development and application of a selective pcr-denaturing gradient gel electrophoresis approach to detect a recently cultivated Bacillus group predominant in soil (2004)

Tzeneva, V.A., Li, Y., Felske, A., Akkermans, A.D.L., Vaughan, E.E., ...

The worldwide presence of a hitherto-nondescribed group of predominant soil microorganisms related to Bacillus benzoevorans was analyzed after development of two sets of selective primers targeting...

Specific response of a novel and abundant Lactobacillus amylovorus-like phylotype to dietary prebiotics in the guts of weaning piglets (2004)

Konstantinov, S.R., Awati, A.A., Smidt, H., Williams, B.A., Akkermans, A.D.L.

Isolation and biodiversity of hitherto undescribed soil bacteria related to Bacillus niacini (2004)

Felske, A., Tzeneva, V.A., Heyrman, J., Langeveld, M.A., Akkermans, A.D.L., Vos, P.

The hitherto largely not described phylogenetic neighborhood of Bacillus niacini has been explored by a comprehensive cultivation experiment and genomic variety studies. Previous culture-independent...

Intracellular proliferation of Legionella pneumophila in Hartmannella vermiformis in aquatic biofilms grown on plasticized polyvinyl chloride (2004)

Kuiper, M.W., Wullings, B.A., Akkermans, A.D.L., Beumer, R.R.

The need for protozoa for the proliferation of Legionella pneumophila in aquatic habitats is still not fully understood and is even questioned by some investigators. This study shows the in vivo...

Insertion-Sequence-Mediated Mutations Isolated During Adaptation to Growth and Starvation in Lactococcus lactis. (2004)

Akkermans, A.D.L., Hoekstra, R.F.

We studied the activity of three multicopy insertion sequence (IS) elements in 12 populations of Lactococcus lactis IL1403 that evolved in the laboratory for 1000 generations under various...

Analysis of rumen bacterial diversity of goat by denaturing gradient electrophoresis and 16S rDNA sequencing (2004)

Yao, W., Han, Z.K., Akkermans, A.D.L., Williams, B.A., Tamminga, S.

Daidzein increased the density but not composition of Lactobacillus community in piglet digesta during in vitro fermentation as revealed by DGGE and dilution PCR (2004)

Yao, W., Han, Z.K., Williams, B.A., Tamminga, S., Akkermans, A.D.L.

Direct ribosome isolation from soil (2004)

Felske, A., Backhaus, H., Akkermans, A.D.L.

DNA extraction from actinorhizal nodules (2004)

Ramirez-Saad, H.R., Akkermans, A.D.L.

The celB marker gene (2004)

Sessitsch, A., Wilson, K.J., Akkermans, A.D.L.

In vitro assessment of GIT fermentation: fermentable substrates and microbial activity (2004)

Williams, B.A., Bosch, M.W., Awati, A.A., Konstantinov, S.R., Akkermans, A.D.L., Verstegen, M.W.A.

Molecular microbial ecology manual (2004)

Kowalchuk, G.A., Head, I.M., Akkermans, A.D.L.

Molecular diversity in the bacterial community and the fluorescent pseudomonads group in natural and chlorobenzoate-stressed peat-forest soil (2003)

Ramirez-Saad, H.C., Sessitsch, A., Akkermans, A.D.L.

Bacterial community shifts in a soil microcosm spiked with 3-chlorobenzoate or 2,5-dichlorobenzoate were monitored. The V6-V8 variable regions of soil bacterial 16S rRNA and rDNA were amplified and...

Effect of fermentable carbohydrates on piglet faecal bacterial communities as revealed by DGGE analysis of 16S rDNA (2003)

Konstantinov, S.R., Williams, B.A., Tamminga, S., Akkermans, A.D.L.

The effect of fermentable carbohydrates (sugar beet pulp and fructooligosaccharides) on the faecal bacterial communities of weaning piglets was analysed using 16S rDNA-based approaches. Amplicons of...

Victivallis vadensis gen. nov., sp. nov., a sugar-fermenting anaerobe from human faeces (2003)

Zoetendal, E.G., Plugge, C.M., Akkermans, A.D.L.

High-throughput PCR Screening of Genes for Three-component Regulatory System Putatively involved in Quorum Sensing from Low-G+C Gram-positive Bacteria (2003)

Nakayama, J., Akkermans, A.D.L.

On-farm impact of cattle slurry manure management on biological soil quality (2003)

Brussaard, L., Akkermans, A.D.L.

The effects of dairy cattle slurry management on soil biota, soil respiration and nitrogen (N) mineralization were evaluated in a farm trial across 12 farms and a field experiment on 2 farms located...

Studies on the potential effects of tyrosol and p-coumaric acid on the human gastro-intestinal tract microflora (2003)

Tassiopoulou, S., Williams, B.A., Vaughan, E.E., Akkermans, A.D.L.

The effect of dietary fermentable carbohydrates on lactic acid concentration and presence of Lactobacillus spp. in small intestinal digesta of piglets until 10 days after weaning (2003)

Williams, B.A., Awati, A.A., Bosch, M.W., Konstantinov, S.R., Akkermans, A.D.L.

The effect of dietary fermentable carbohydrates on lactic acid concentration in small intestinal digesta of weaning piglets at ten days after weaning (2003)

Williams, B.A., Awati, A.A., Bosch, M.W., Konstantinov, S.R., Akkermans, A.D.L.

Selective enrichment of Geobacter sulfurreducens from anaerobic granular sludge with quinones as terminal electron acceptors (2003)

Cervantes-Carillo, F.J., Duong Dac, T., Ivanova, A.E., Akkermans, A.D.L., Lettinga, G., ...

Image analysis, methanogenic activity measurements, and molecular biological techniques to monitor granular sludge from EGSB reactors fed with oleic acid (2003)

Pereira, M.A., Stams, A.J.M., Akkermans, A.D.L., Amaral, A.L., Pons, M-N., ...

Enrichment and immobilization of quinone-respiring bacteria in anaerobic granular sludge (2003)

Cervantes-Carillo, F.J., Duong-Dac, T., Akkermans, A.D.L., Lettinga, G., Field, J.A.

The capacity of an anaerobic granular sludge for serving as an immobilizing mechanism for quinone-respiring bacteria was evaluated. The inoculum was continuously fed with a basal medium containing...

Development of bacterial and bifidobacterial communities in feces of newborn babies (2003)

Favier, C.F., Akkermans, A.D.L.

Bacterial gene expression detected in human faeces by reverse transcription-PCR (2003)

Fitzsimons, N.A., Akkermans, A.D.L., Vaughan, E.E.

Sponge cell culture? A molecular identification method for sponge cells (2003)

Sipkema, D., Heilig, G.H.J., Akkermans, A.D.L., Osinga, R., Tramper, J., Wijffels, R.H.

Analysis of 16S rDNA reveals bacterial shift during in vitro fermentation of fermentable carbohydrate using piglet faeces as inoculum (2003)

Williams, B.A., Konstantinov, S.R., Tamminga, S., Akkermans, A.D.L.

Image analysis, methanogenic activity measurements, and molecular biological techniques to monitor granular sludge from an EGSB reactor fed with oleic acid (2003)

Pereira, M. A., Roest, K., Stams, A. J. M., Akkermans, A. D. L., Amaral, A. L., Pons, M. N., ...

Morphological changes in anaerobic granular sludge fed with increasing loads of oleic acid were quantified by image analysis. The combination of this technique with data on the accumulation of...

Image analysis, methanogenic activity and molecular biological techniques to monitor granular sludge from an egsb reactor fed with oleic acid (2003)

Pereira, M. A., Roest, K., Stams, A. J. M., Akkermans, A. D. L., Amaral, A. L., Pons, M. N., ...

Morphological changes in anaerobic granular sludge fed with increasing loads of oleic acid were quantified by image analysis. The combination of this technique with data on the accumulation of...

Molecular monitoring of succession of bacterial communities in human neonates (2002)

Favier, C., Vaughan, E.E., Akkermans, A.D.L.

The establishment of bacterial communities in two healthy babies was examined for more than the first 10 months of life by monitoring 16S ribosomal DNA (rDNA) diversity in fecal samples by PCR and...

Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA (2002)

Heilig, G.H.J., Zoetendal, E.G., Vaughan, E.E., Marteau, P., Akkermans, A.D.L.

A Lactobacillus group-specific PCR primer, S-G-Lab-0677-a-A-17, was developed to selectively amplify 16S ribosomal DNA (rDNA) from lactobacilli and related lactic acid bacteria, including members of...

From composition to functionality of the intestinal microbial communities (2002)

Konstantinov, S., Fitzsimons, N., Vaughan, E.E., Akkermans, A.D.L.

Molecular monitoring of microbial diversity in expanded granular sludge bed (EGSB) reactors treating oleic acid. FEMS (2002)

Pereira, M.A., Roest, C., Stams, A.J.M., Alves, M.M., Akkermans, A.D.L.

A molecular approach was used to evaluate the microbial diversity of bacteria and archaea in two expanded granular sludge bed (EGSB) reactors fed with increasing oleic acid loading rates up to 8 kg...

Diversity, Dynamics and Activity of Bacterial Communities during Production of an Artisanal Sicilian Cheese as Evaluated by 16S rRNA Analysis (2002)

Randazzo, C.L., Torriani, S., Akkermans, A.D.L., Vaughan, E.E.

The diversity and dynamics of the microbial communities during the manufacturing of Ragusano cheese, an artisanal cheese produced in Sicily (Italy), were investigated by a combination of classical...

The intestinal LABs (2002)

Vaughan, E.E., Zoetendal, E.G., Ben-Amor, K., Akkermans, A.D.L.

Quantification of uncultured Ruminococcus obeum-like bacteria in human fecal samples with fluorescent in situ hybridization and flow cytometry using 16S ribosomal RNA targeted probes (2002)

Zoetendal, E.G., Ben-Amor, K., Harmsen, H.J.M., Schut, F., Akkermans, A.D.L.

A 16S rRNA-targeted probe was designed and validated in order to quantify the number of uncultured Ruminococcus obeum-like bacteria by fluorescent in situ hybridization (FISH). These bacteria have...

Mucosa-associated bacteria in the human gastrointestinal tract are uniformly distributed along the colon and differ from the community recovered from feces (2002)

Zoetendal, E.G., Vilpponen-Salmela, T., Amor, B., Akkermans, A.D.L.

The human gastrointestinal (GI) tract harbors a complex community of bacterial cells in the mucosa, lumen, and feces. Since most attention has been focused on bacteria present in feces, knowledge...

Bacterial translocation in weaning piglets analysed by 16s rDNA based approaches (2002)

Konstantinov, S.R., Leser, T.D., Williams, B.A., Akkermans, A.D.L.

Cell to cell communication by autoinducing in gram-positive bacteria (2002)

Sturme, M.H.J., Kleerebezem, M., Nakayama, J., Akkermans, A.D.L., Vaughan, E.E.

While intercellular communication systems in Gram-negative bacteria are often based on homoserine lactones as signalling molecules, it has been shown that autoinducing peptides are involved in...

Molecular microbial ecology manual : suppl. 5 (2002)

Akkermans, A.D.L.

Bacterial translocation in weaning piglets analyzed by 16S rDNA based approaches (2002)

Konstantinov, S.R., Leser, T.D., Williams, B.A., Akkermans, A.D.L.

Multiparametric flow cytometry and cell sorting for the assessment of viable, injured, and dead bifidobacterium cells during bile salt stress (2002)

Ben Amor, K., Breeuwer, P., Verbaarschot, P., Rombouts, F.M., Akkermans, A.D.L., ...

Using a flow cytometry-based approach, we assessed the viability of Bifidobacterium lactis DSM 10140 and Bifidobacterium adolescentis DSM 20083 during exposure to bile salt stress. Carboxyfluorescein...

Selective enrichment of Geobacter sulfurreducens from anaerobic granular sludge with quinones as terminal electron acceptors (2002)

Cervantes, F.J., Duong-Dac, T., Ivanova, A., Roest, K., Akkermans, A.D.L., Lettinga, G., ...

A quinone-respiring, enrichment culture derived from methanogenic granular sludge was phylogenetically characterized by using a combined cloning-denaturing gradient gel electrophoresis (DGGE) method,...

Molecular monitoring of microbial diversity in expanded granular sludge bed (EGSB) reactors treating oleic acid (2002)

Pereira, M. A., Roest, K., Stams, A. J. M., Mota, M., Alves, M. M., Akkermans, A. D. L.

A molecular approach was used to evaluate the microbial diversity of bacteria and archaea in two expanded granular sludge bed (EGSB) reactors fed with increasing oleic acid loading rates up to 8 kg...

Molecular characterization of key enzymes in halorespiration. (2001)

Smidt, H., Akkermans, A.D.L.

Culture-independent detection of bacterial populations in the human intestinal tract (2001)

Akkermans, A.D.L., Zoetendal, E.G., Konstantinov, S., Favier, C., Satokari, R., Vaughan, E.E., ...

Gelatinase biosynthesis-activating pheromone: a peptide lactone that mediates a quorum sensing in Enterococcus faecalis (2001)

Nakayama, J., Cao, Y., Horii, T., Sakuda, S., Akkermans, A.D.L., ...

Biosynthesis of gelatinase, a virulence factor of Enterococcus faecalis, was found to be regulated in a cell density-dependent fashion in which its production is active in late log to early...

Bifidobacterial diversity in human feces detected by genus-specific polymerase chain reaction and denaturing gradient gel electrophoresis (2001)

Satokari, R.M., Vaughan, E.E., Akkermans, A.D.L., Saarela, M.

We describe the development and validation of a method for the qualitative analysis of complex bifidobacterial communities based on PCR and denaturing gradient gel electrophoresis (DGGE)....

Polymerase chain reaction and denaturing gradient gel electrophoresis monitoring of fecal Bifidobacterium populations in a prebiotic and probiotic feeding trial (2001)

Satokari, R.M., Vaughan, E.E., Akkermans, A.D.L.

A culture-independent approach based on genus-specific PCR and denaturing gradient gel electrophoresis (DGGE) was used to monitor qualitative changes in fecal bifidobacterial communities in a human...

The host genotype affects the bacterial community in the human gastrointestinal tract (2001)

Zoetendal, E.G., Akkermans, A.D.L.

The gastrointestinal (GI) tract is one of the most complex ecosystems consisting of microbial and host cells. It is suggested that the host genotype, the physiology of the host and environmental...

Molecular characterization of microbial communities in the GI-tract based on 16S rRNA sequence diversity (2001)

Zoetendal, E.G., Akkermans, A.D.L.

DNA isolation protocols affect the detection limit of PCR approaches of bacteria in samples from the human gastrointestinal tract (2001)

Zoetendal, E.G., Ben-Amor, K., Akkermans, A.D.L., Abee, T.

A major concern in molecular ecological studies is the lysis efficiency of different bacteria in a complex ecosystem. We used a PCR-based 16S rDNA approach to determine the effect of two DNA...

Performance and Microbial Composition of Different Anaerobic Reactor Configurations Treating High Ammonia Landfill Leachate (2001)

Inanc, B., Calli, B., Mertoglu, B., Roest, K., Akkermans, A.D.L.

The host genotype affects the bacterial community in the human gastrointestinal tract (2001)

Zoetendal, E.G., Akkermans, A.D.L.

The gastrointestinal (GI) tract is one of the most complex ecosystems consisting of microbial and host cells. It is suggested that the host genotype, the physiology of the host and environmental...

Molecular characterization of microbial communities in the GI-tract based on 16S rRNA sequence diversity (2001)

Zoetendal, E.G., Akkermans, A.D.L.

DNA isolation protocols affect the detection limit of PCR approaches of bacteria in samples from the human gastrointestinal tract (2001)

Zoetendal, E.G., Ben-Amor, K., Akkermans, A.D.L., Abee, T.

A major concern in molecular ecological studies is the lysis efficiency of different bacteria in a complex ecosystem. We used a PCR-based 16S rDNA approach to determine the effect of two DNA...

Microbial colonization of the mammalian intestine : a molecular approach (2001)

Akkermans, A.D.L., Zoetendal, E.G., Favier, C., Konstantinov, S., Williams, B.A.

Temperature and denaturating gradient gel electrophoresis analysis of 16S rRNA from human faecal samples (2000)

Akkermans, A.D.L., Zoetendal, E.G., Favier, C.F.

Response of a soil bacterial community to grassland succession as monitored by 16S rRNA levels of the predominant ribotypes (2000)

Felske, A., Wolterink, A., Akkermans, A.D.L.

The composition of predominant soil bacteria during grassland succession was investigated in the Dutch Drentse A area. Five meadows, taken out of agricultural production at different time points, and...

Spatial distribution of 16S rRNA levels from uncultured acidobacteria in soil (2000)

Felske, A., Akkermans, A.D.L.

Halorespiring bacteria-molecular characterization and detection (2000)

Smidt, H., Akkermans, A.D.L.

A molecular view of the intestinal ecosystem (2000)

Vaughan, E.E., Schut, F., Heilig, G.H.J., Zoetendal, E.G., Akkermans, A.D.L.

This review describes the state of the art as well as the initial results of molecular methodologies used to study the ecology of the complex microflora of the human intestinal tract. The detection...