Location of the active site for enzyme-adenylate formation in DNA ligases.
Tomkinson, A E, Totty, N F, Ginsburg, M, Lindahl, T
The enzyme-AMP reaction intermediate of the 102-kDa bovine DNA ligase I was digested with trypsin, and the adenylylated peptide was isolated by chromatography under conditions that maintain the...
Human DNA ligase I cDNA: cloning and functional expression in Saccharomyces cerevisiae.
Barnes, D E, Johnston, L H, Kodama, K, Tomkinson, A E, Lasko, D D, Lindahl, T
Human cDNA clones encoding the major DNA ligase activity in proliferating cells, DNA ligase I, were isolated by two independent methods. In one approach, a human cDNA library was screened by...
Two distinct DNA ligase activities in mitotic extracts of the yeast Saccharomyces cerevisiae.
Ramos, W, Tappe, N, Talamantez, J, Friedberg, E C, Tomkinson, A E
Four biochemically distinct DNA ligases have been identified in mammalian cells. One of these enzymes, DNA ligase I, is functionally homologous to the DNA ligase encoded by the Saccharomyces...
Karimi-Busheri, F, Lee, J, Tomkinson, A E, Weinfeld, M
A putative role for mammalian polynucleotide kinases that possess both 5'-phosphotransferase and 3'-phosphatase activity is the restoration of DNA strand breaks with 5'-hydroxyl termini or...
Ramos, W, Liu, G, Giroux, C N, Tomkinson, A E
Here we demonstrate that the Saccharomyces cerevisiae DNA ligase activity, which we previously designated DNA ligase II, is encoded by the genomic DNA sequence YOR005c. Based on its homology with...
Chen, J, Tomkinson, A E, Ramos, W, Mackey, Z B, Danehower, S, Walter, C A, ...
Three biochemically distinct DNA ligase activities have been identified in mammalian cell extracts. We have recently purified DNA ligase II and DNA ligase III to near homogeneity from bovine liver...
Mackey, Z B, Ramos, W, Levin, D S, Walter, C A, McCarrey, J R, Tomkinson, A E
Three mammalian genes encoding DNA ligases have been identified. However, the role of each of these enzymes in mammalian DNA metabolism has not been established. In this study, we show that two forms...
Tomkinson, A E, Starr, R, Schultz, R A
The phenotypes of cultured cell lines established from individuals with Bloom syndrome (BLM), including an elevated spontaneous frequency of sister chromatid exchanges (SCEs), are consistent with a...
Finch, P W, Wilson, R E, Brown, K, Hickson, I D, Tomkinson, A E, Emmerson, P T
The nucleotide sequence of a 6,000 bp region of the E. coli chromosome that includes the 3' end of the coding region for the thyA gene and the entire recC gene has been determined. The proposed...
Molecular amplification and purification of the E. coli recC gene product.
Hickson, I D, Atkinson, K E, Hutton, L, Tomkinson, A E, Emmerson, P T
The level of recC gene expression has been analysed using Mud(bla lac) fusions to the recC promoter. The constitutive level of expression is very low and remains so even under SOS inducing...
Mammalian mitochondrial endonuclease activities specific for ultraviolet-irradiated DNA.
Tomkinson, A E, Bonk, R T, Kim, J, Bartfeld, N, Linn, S
Mitochondrial forms of uracil DNA glycosylase and UV endonuclease have been purified and characterized from the mouse plasmacytoma cell line, MPC-11. As in other cell types, the mitochondrial uracil...
Purification and properties of a single strand-specific endonuclease from mouse cell mitochondria.
A nuclease was purified from mitochondria of the mouse plasmacytoma cell line, MCP-11 which acts on single-stranded DNA endonucleolytically and appears to have no activity upon native DNA. It...
Montecucco, A, Rossi, R, Levin, D S, Gary, R, Park, M S, Motycka, T A, ...
In mammalian cells, DNA replication occurs at discrete nuclear sites termed replication factories. Here we demonstrate that DNA ligase I and the large subunit of replication factor C (RF-C p140) have...
Location of the active site for enzyme-adenylate formation in DNA ligases.
Tomkinson, A E, Totty, N F, Ginsburg, M, Lindahl, T
The enzyme-AMP reaction intermediate of the 102-kDa bovine DNA ligase I was digested with trypsin, and the adenylylated peptide was isolated by chromatography under conditions that maintain the...
Human DNA ligase I cDNA: cloning and functional expression in Saccharomyces cerevisiae.
Barnes, D E, Johnston, L H, Kodama, K, Tomkinson, A E, Lasko, D D, Lindahl, T
Human cDNA clones encoding the major DNA ligase activity in proliferating cells, DNA ligase I, were isolated by two independent methods. In one approach, a human cDNA library was screened by...
Two distinct DNA ligase activities in mitotic extracts of the yeast Saccharomyces cerevisiae.
Ramos, W, Tappe, N, Talamantez, J, Friedberg, E C, Tomkinson, A E
Four biochemically distinct DNA ligases have been identified in mammalian cells. One of these enzymes, DNA ligase I, is functionally homologous to the DNA ligase encoded by the Saccharomyces...
Karimi-Busheri, F, Lee, J, Tomkinson, A E, Weinfeld, M
A putative role for mammalian polynucleotide kinases that possess both 5'-phosphotransferase and 3'-phosphatase activity is the restoration of DNA strand breaks with 5'-hydroxyl termini or...
Ramos, W, Liu, G, Giroux, C N, Tomkinson, A E
Here we demonstrate that the Saccharomyces cerevisiae DNA ligase activity, which we previously designated DNA ligase II, is encoded by the genomic DNA sequence YOR005c. Based on its homology with...
Chen, J, Tomkinson, A E, Ramos, W, Mackey, Z B, Danehower, S, Walter, C A, ...
Three biochemically distinct DNA ligase activities have been identified in mammalian cell extracts. We have recently purified DNA ligase II and DNA ligase III to near homogeneity from bovine liver...
Mackey, Z B, Ramos, W, Levin, D S, Walter, C A, McCarrey, J R, Tomkinson, A E
Three mammalian genes encoding DNA ligases have been identified. However, the role of each of these enzymes in mammalian DNA metabolism has not been established. In this study, we show that two forms...
Tomkinson, A E, Starr, R, Schultz, R A
The phenotypes of cultured cell lines established from individuals with Bloom syndrome (BLM), including an elevated spontaneous frequency of sister chromatid exchanges (SCEs), are consistent with a...
Finch, P W, Wilson, R E, Brown, K, Hickson, I D, Tomkinson, A E, Emmerson, P T
The nucleotide sequence of a 6,000 bp region of the E. coli chromosome that includes the 3' end of the coding region for the thyA gene and the entire recC gene has been determined. The proposed...
Molecular amplification and purification of the E. coli recC gene product.
Hickson, I D, Atkinson, K E, Hutton, L, Tomkinson, A E, Emmerson, P T
The level of recC gene expression has been analysed using Mud(bla lac) fusions to the recC promoter. The constitutive level of expression is very low and remains so even under SOS inducing...
Mammalian mitochondrial endonuclease activities specific for ultraviolet-irradiated DNA.
Tomkinson, A E, Bonk, R T, Kim, J, Bartfeld, N, Linn, S
Mitochondrial forms of uracil DNA glycosylase and UV endonuclease have been purified and characterized from the mouse plasmacytoma cell line, MPC-11. As in other cell types, the mitochondrial uracil...
Purification and properties of a single strand-specific endonuclease from mouse cell mitochondria.
A nuclease was purified from mitochondria of the mouse plasmacytoma cell line, MCP-11 which acts on single-stranded DNA endonucleolytically and appears to have no activity upon native DNA. It...
Montecucco, A, Rossi, R, Levin, D S, Gary, R, Park, M S, Motycka, T A, ...
In mammalian cells, DNA replication occurs at discrete nuclear sites termed replication factories. Here we demonstrate that DNA ligase I and the large subunit of replication factor C (RF-C p140) have...