Bushman, F D, Engelman, A, Palmer, I, Wingfield, P, Craigie, R
The integrase protein of human immunodeficiency virus type 1 carries out a set of polynucleotidyl transfer reactions that result in the covalent attachment of the retroviral cDNA to host DNA. We have...
Multiple effects of mutations in human immunodeficiency virus type 1 integrase on viral replication.
Engelman, A, Englund, G, Orenstein, J M, Martin, M A, Craigie, R
The integration of a DNA copy of the human immunodeficiency virus type 1 (HIV-1) genome into a chromosome of an infected cell is a pivotal step in virus replication. Integration requires the activity...
Englund, G, Theodore, T S, Freed, E O, Engelman, A, Martin, M A
Certain human immunodeficiency virus type 1 (HIV-1) isolates are able to productively infect nondividing cells of the monocyte/macrophage lineage. We have used a molecular genetic approach to...
Efficient magnesium-dependent human immunodeficiency virus type 1 integrase activity.
The integrase protein from human immunodeficiency virus type 1 (HIV-1) has generally been reported to require Mn2+ for efficient in vitro activity. We have reexamined the divalent metal ion...
Taddeo, B, Carlini, F, Verani, P, Engelman, A
The integration of a DNA copy of the retroviral RNA genome into the host cell genome is essential for viral replication. The virion-associated integrase protein, encoded by the 3' end of the viral...
Engelman, A, Liu, Y, Chen, H, Farzan, M, Dyda, F
Two different crystal structures of the human immunodeficiency virus type 1 (HIV-1) integrase (IN) catalytic domain were analyzed for interactions at the enzyme active site. Gln-62 and Glu-92...
A mutation in integrase can compensate for mutations in the simian immunodeficiency virus att site.
Du, Z, Ilyinskii, P O, Lally, K, Desrosiers, R C, Engelman, A
Sequences at the left terminus of U3 in the left long terminal repeat (LTR) and at the right terminus of U5 in the right LTR are important for integration of retroviral DNA. In the infectious...
Engelman, A, Hickman, A B, Craigie, R
The integrase protein of human immunodeficiency virus type 1 removes two nucleotides from the 3' ends of reverse-transcribed human immunodeficiency virus type 1 DNA (3' processing) and covalently...
Vicenzi, E, Dimitrov, D S, Engelman, A, Migone, T S, Purcell, D F, Leonard, J, ...
Nonoverlapping deletions that eliminated the 5' (HIV-1US/603del), middle (HIV-1U5/206del), and 3' (HIV-1U5/604del) thirds of the U5 region of the human immunodeficiency virus type 1 (HIV-1) long...
We have probed the structural organization of the human immunodeficiency virus type 1 integrase protein by limited proteolysis and the functional organization by site-directed mutagenesis of selected...
The effect of two missense mutations in abl on transformation by Abelson murine leukemia virus was evaluated. These mutations led to the substitution of a histidine for Tyr-590 and a glycine for...
Isolation of temperature-sensitive Abelson virus mutants by site-directed mutagenesis.
Mutants of Abelson virus encoding temperature-sensitive protein-tyrosine kinase (EC 2.7.1.112) were created by site-directed mutagenesis using sequence information from temperature-sensitive mutants...
Mazumder, A, Engelman, A, Craigie, R, Fesen, M, Pommier, Y
We report the activities of HIV integrase protein on a novel DNA substrate, consisting of a pair of gapped duplex molecules. Integrase catalyzed an intermolecular disintegration reaction that...
A rapid in vitro assay for HIV DNA integration.
Craigie, R, Mizuuchi, K, Bushman, F D, Engelman, A
Retroviruses synthesize a double stranded DNA copy of their RNA genome after infection of a permissive cell and subsequent integration of this DNA copy into the host genome is necessary for normal...
bcr/abl and src but not myc and ras replace v-abl in lymphoid transformation.
Lymphoid cells transformed by temperature-sensitive Abelson virus die at the nonpermissive temperature. This property was exploited to show that bcr/abl and v-src but not myc and ras can replace the...
Engelman, A, Bushman, F D, Craigie, R
HIV-1 integrase protein possesses the 3' processing and DNA strand transfer activities that are required to integrate HIV DNA into a host chromosome. The N-, C-terminal and core domains of integrase...
Jenkins, T M, Esposito, D, Engelman, A, Craigie, R
Analysis of the crystal structure of HIV-1 integrase reveals a cluster of lysine residues near the active site. Using site-directed mutagenesis and photo-crosslinking we find that Lys156 and Lys159...
Bushman, F D, Engelman, A, Palmer, I, Wingfield, P, Craigie, R
The integrase protein of human immunodeficiency virus type 1 carries out a set of polynucleotidyl transfer reactions that result in the covalent attachment of the retroviral cDNA to host DNA. We have...
Multiple effects of mutations in human immunodeficiency virus type 1 integrase on viral replication.
Engelman, A, Englund, G, Orenstein, J M, Martin, M A, Craigie, R
The integration of a DNA copy of the human immunodeficiency virus type 1 (HIV-1) genome into a chromosome of an infected cell is a pivotal step in virus replication. Integration requires the activity...
Englund, G, Theodore, T S, Freed, E O, Engelman, A, Martin, M A
Certain human immunodeficiency virus type 1 (HIV-1) isolates are able to productively infect nondividing cells of the monocyte/macrophage lineage. We have used a molecular genetic approach to...
Efficient magnesium-dependent human immunodeficiency virus type 1 integrase activity.
The integrase protein from human immunodeficiency virus type 1 (HIV-1) has generally been reported to require Mn2+ for efficient in vitro activity. We have reexamined the divalent metal ion...
Taddeo, B, Carlini, F, Verani, P, Engelman, A
The integration of a DNA copy of the retroviral RNA genome into the host cell genome is essential for viral replication. The virion-associated integrase protein, encoded by the 3' end of the viral...
Engelman, A, Liu, Y, Chen, H, Farzan, M, Dyda, F
Two different crystal structures of the human immunodeficiency virus type 1 (HIV-1) integrase (IN) catalytic domain were analyzed for interactions at the enzyme active site. Gln-62 and Glu-92...
A mutation in integrase can compensate for mutations in the simian immunodeficiency virus att site.
Du, Z, Ilyinskii, P O, Lally, K, Desrosiers, R C, Engelman, A
Sequences at the left terminus of U3 in the left long terminal repeat (LTR) and at the right terminus of U5 in the right LTR are important for integration of retroviral DNA. In the infectious...
Engelman, A, Hickman, A B, Craigie, R
The integrase protein of human immunodeficiency virus type 1 removes two nucleotides from the 3' ends of reverse-transcribed human immunodeficiency virus type 1 DNA (3' processing) and covalently...
Vicenzi, E, Dimitrov, D S, Engelman, A, Migone, T S, Purcell, D F, Leonard, J, ...
Nonoverlapping deletions that eliminated the 5' (HIV-1US/603del), middle (HIV-1U5/206del), and 3' (HIV-1U5/604del) thirds of the U5 region of the human immunodeficiency virus type 1 (HIV-1) long...
We have probed the structural organization of the human immunodeficiency virus type 1 integrase protein by limited proteolysis and the functional organization by site-directed mutagenesis of selected...
The effect of two missense mutations in abl on transformation by Abelson murine leukemia virus was evaluated. These mutations led to the substitution of a histidine for Tyr-590 and a glycine for...
Isolation of temperature-sensitive Abelson virus mutants by site-directed mutagenesis.
Mutants of Abelson virus encoding temperature-sensitive protein-tyrosine kinase (EC 2.7.1.112) were created by site-directed mutagenesis using sequence information from temperature-sensitive mutants...
Mazumder, A, Engelman, A, Craigie, R, Fesen, M, Pommier, Y
We report the activities of HIV integrase protein on a novel DNA substrate, consisting of a pair of gapped duplex molecules. Integrase catalyzed an intermolecular disintegration reaction that...
A rapid in vitro assay for HIV DNA integration.
Craigie, R, Mizuuchi, K, Bushman, F D, Engelman, A
Retroviruses synthesize a double stranded DNA copy of their RNA genome after infection of a permissive cell and subsequent integration of this DNA copy into the host genome is necessary for normal...
bcr/abl and src but not myc and ras replace v-abl in lymphoid transformation.
Lymphoid cells transformed by temperature-sensitive Abelson virus die at the nonpermissive temperature. This property was exploited to show that bcr/abl and v-src but not myc and ras can replace the...
Engelman, A, Bushman, F D, Craigie, R
HIV-1 integrase protein possesses the 3' processing and DNA strand transfer activities that are required to integrate HIV DNA into a host chromosome. The N-, C-terminal and core domains of integrase...
Jenkins, T M, Esposito, D, Engelman, A, Craigie, R
Analysis of the crystal structure of HIV-1 integrase reveals a cluster of lysine residues near the active site. Using site-directed mutagenesis and photo-crosslinking we find that Lys156 and Lys159...