CHARGE EMISSION AND RELATED PRECURSOR EVENTS ASSOCIATED WITH LASER DAMAGE. (9999)
BECKER ,M. F., DOMANN ,F. E., STEWART ,A. F., GUENTHER,A. H.
The European dimension for the mouse genome mutagenesis program (2008)
Auwerx, J., Avner, P., Baldock, R., Ballabio, A., Balling, R., Barbacid, M., ...
The European Mouse Mutagenesis Consortium is the European initiative contributing to the international effort on functional annotation of the mouse genome. Its objectives are to establish and...
Nedelcheva, M. N., Roguev, A., Dolapchiev, L. B., Shevchenko, A., Taskov, H. B., Shevchenko, A., ...
Voziyanov, Y., Koneiczka, J. H., Stewart, A. F., Jayaram, M.
The Flp protein from Saccharomyces cerevisiae is one of the site-specific tyrosine family recombinases that are used widely in genomic engineering. As a first step towards mediating directed DNA...
Voziyanov, Y., Stewart, A. F., Jayaram, M.
We have developed a dual reporter screen in Escherichia coli for identifying variants of the Flp site-specific recombinase that have acquired reactivity at an altered target site (mFRT). In one...
Anastassiadis, K., Kim, J., Daigle, N., Prengel, R., Schöler, H. R., Stewart, A. F.
Several strategies for regulated stable transgene expression in mammalian cells have been described. These strategies have different strengths and weaknesses, however they all share a common problem,...
Shevchenko, A., Schaft, D., Roguev, A., Stewart, A. F.
We employed a combination of tandem affinity purification and mass spectrometry for deciphering protein complexes and the protein interaction network in budding yeast. 53 genes were epitope-tagged,...
Hormonal and liver-specific control of expression of the tyrosine aminotransferase gene (1991)
Nitsch, Doris, Ruppert, S., Kelsey, G. D., Schedl, A., Weih, Falk, Stewart, A. F., ...
Nitsch, Doris, Stewart, A. F., Boshart, Michael, Mestril, Ruben, Weih, Falk, Schütz, Günther
The relationship between DNase I-hypersensitive sites (HSs) and transcriptional enhancers of the rat tyrosine aminotransferase (TAT) gene was examined by comparing HSs in and around the TAT gene with...
In vivo monitoring of a cAMP-stimulated DNA-binding activity (1990)
Weih, Falk, Stewart, A. F., Boshart, Michael, Nitsch, Doris, Schlitz, Günther
Ligand-regulated site-specific recombination.
Site-specific recombination offers a potential way to alter a living genome by design in a precise and stable manner. This potential requires strategies which can be used to regulate the...
At least three promoters direct expression of the mouse glucocorticoid receptor gene.
Strähle, U, Schmidt, A, Kelsey, G, Stewart, A F, Cole, T J, Schmid, W, ...
We have characterized the gene for the mouse glucocorticoid receptor. The gene spans approximately 110 kilobases, and glucocorticoid receptor transcripts are assembled from nine exons. Expression of...
Buchholz, F, Angrand, P O, Stewart, A F
We report the construction of two Escherichia coli strains (294-Cre and 294-FLP) which express either Cre- or FLP-recombinase. Plasmids containing authentic recognition targets for either recombinase...
Buchholz, F, Ringrose, L, Angrand, P O, Rossi, F, Stewart, A F
Genomic manipulations using site-specific recombinases rely on their applied characteristics in living systems. To understand their applied properties so that they can be optimally deployed, we...
Temporally and spatially regulated somatic mutagenesis in mice.
Schwenk, F, Kuhn, R, Angrand, P O, Rajewsky, K, Stewart, A F
In mice transgenesis through oocyte injection or DNA recombination in embryonal stem (ES) cells allows mutations to be introduced into the germline. However, the earliest phenotype of the introduced...
Angrand, P O, Woodroofe, C P, Buchholz, F, Stewart, A F
When fused to the ligand binding domain (LBD) of steroid hormone nuclear receptors, site-specific recombinases (SSRs) acquire a ligand-dependent activity. Here, we describe the use of SSR-LBD fusion...
Rapid modification of bacterial artificial chromosomes by ET-recombination.
Muyrers, J P, Zhang, Y, Testa, G, Stewart, A F
We present a method to modify bacterial artificial chromosomes (BACs) resident in their host strain. The method is based on homologous recombination by ET-cloning. We have successfully modified BACs...
The chicken HPRT gene: a counter selectable marker for the DT40 cell line.
Fukagawa, T, Hayward, N, Yang, J, Azzalin, C, Griffin, D, Stewart, A F, ...
We describe the cloning, characterisation and chromosomal mapping of the chicken hprt gene together with the construction of two counter selectable hprt-/- DT40 derived cell lines. One of these cell...
Simplified generation of targeting constructs using ET recombination.
Angrand, P O, Daigle, N, Schöler, H R, Stewart, A F
ET recombination is a way to engineer DNA in Escherichia coli using homologous recombination. Here we develop the potential of ET recombination in two ways relevant to complex engineering exercises...
Mangin, M, Webb, A C, Dreyer, B E, Posillico, J T, Ikeda, K, Weir, E C, ...
Humoral hypercalcemia of malignancy is a common paraneoplastic syndrome that appears to be mediated in many instances by a parathyroid hormone-like peptide. Poly(A)+ RNA from a human renal carcinoma...
Soifer, N E, Van Why, S K, Ganz, M B, Kashgarian, M, Siegel, N J, Stewart, A F
Parathyroid hormone-related protein (PTHrP) is widely expressed in normal adult and fetal tissues, where it acts in an autocrine/paracrine fashion, stimulates growth and differentiation, and shares...
Insogna, K L, Stewart, A F, Morris, C A, Hough, L M, Milstone, L M, Centrella, M
A human parathyroid-like protein (PLP) has recently been isolated and cloned from human tumors associated with the paraneoplastic syndrome, humoral hypercalcemia of malignancy. PLP shares...
The chromo shadow domain, a second chromo domain in heterochromatin-binding protein 1, HP1.
The chromo domain was originally identified as a protein sequence motif common to the Drosophila chromatin proteins, Polycomb (Pc) and heterochromatin protein 1 [HP1; Paro and Hogness (1991) Proc....
Complete sequence of the bovine beta-lactoglobulin cDNA.
Alexander, L J, Hayes, G, Pearse, M J, Beattie, C W, Stewart, A F, Willis, I M, ...
Nucleotide sequences of bovine alpha S1- and kappa-casein cDNAs.
Stewart, A F, Willis, I M, Mackinlay, A G
The nucleotide sequences corresponding to bovine alpha S1- and kappa-casein mRNAs are presented. An unusual alpha S1-casein cDNA has been characterised whose 5' end commences upstream from its...
Stewart, A F, Mangin, M, Wu, T, Goumas, D, Insogna, K L, Burtis, W J, ...
Parathyroid hormone-like adenylate cyclase-stimulating proteins (hACSPs) have been implicated as one of the calcemic, bone-resorbing agents in patients with humoral hypercalcemia of malignancy. We...
Evolution of the casein multigene family: conserved sequences in the 5' flanking and exon regions.
Yu-Lee, L Y, Richter-Mann, L, Couch, C H, Stewart, A F, Mackinlay, A G, Rosen, J M
The rat alpha- and bovine alpha s1-casein genes have been isolated and their 5' sequences determined. The rat alpha-, beta-, gamma- and bovine alpha s1-casein genes contain similar 5' exon...
Nitsch, D, Stewart, A F, Boshart, M, Mestril, R, Weih, F, Schütz, G
The relationship between DNase I-hypersensitive sites (HSs) and transcriptional enhancers of the rat tyrosine aminotransferase (TAT) gene was examined by comparing HSs in and around the TAT gene with...
Rodan, S B, Insogna, K L, Vignery, A M, Stewart, A F, Broadus, A E, D'Souza, S M, ...
The culture media of three cell lines, a human prostate carcinoma (PC3), a rat Leydig cell tumor (Rice-500), and a rat carcinosarcoma (WRC-256), that were derived from tumors associated with humoral...
Stewart, A F, Insogna, K L, Goltzman, D, Broadus, A E
Humoral hypercalcemia of malignancy (HHM) most commonly results from secretion by tumors of an unidentified circulating calcemic factor that appears in clinical studies to stimulate both a...
Ikeda, K, Mangin, M, Dreyer, B E, Webb, A C, Posillico, J T, Stewart, A F, ...
The syndrome of humoral hypercalcemia of malignancy (HHM) appears to be mediated in many instances by a parathyroid hormone-like peptide, which has recently been purified, sequenced, and cloned....
Reik, A, Schütz, G, Stewart, A F
Induction of the rat tyrosine aminotransferase gene (TAT) with glucocorticoid hormones leads to formation of a nuclease hypersensitive site at the hormone-dependent enhancer located 2.5 kb upstream...
Nichols, M, Rientjes, J M, Stewart, A F
The estrogen receptor is capable of binding a diverse set of ligands that are broadly categorized as agonists or antagonists, depending on their abilities to induce or interfere with transcriptional...
Ringrose, L, Chabanis, S, Angrand, P O, Woodroofe, C, Stewart, A F
The probability that two sites on a linear DNA molecule will contact each other by looping depends on DNA flexibility. Although the flexibility of naked DNA in vitro is well characterized, looping in...
Ligand-regulated site-specific recombination.
Site-specific recombination offers a potential way to alter a living genome by design in a precise and stable manner. This potential requires strategies which can be used to regulate the...
At least three promoters direct expression of the mouse glucocorticoid receptor gene.
Strähle, U, Schmidt, A, Kelsey, G, Stewart, A F, Cole, T J, Schmid, W, ...
We have characterized the gene for the mouse glucocorticoid receptor. The gene spans approximately 110 kilobases, and glucocorticoid receptor transcripts are assembled from nine exons. Expression of...
Buchholz, F, Angrand, P O, Stewart, A F
We report the construction of two Escherichia coli strains (294-Cre and 294-FLP) which express either Cre- or FLP-recombinase. Plasmids containing authentic recognition targets for either recombinase...
Buchholz, F, Ringrose, L, Angrand, P O, Rossi, F, Stewart, A F
Genomic manipulations using site-specific recombinases rely on their applied characteristics in living systems. To understand their applied properties so that they can be optimally deployed, we...
Temporally and spatially regulated somatic mutagenesis in mice.
Schwenk, F, Kuhn, R, Angrand, P O, Rajewsky, K, Stewart, A F
In mice transgenesis through oocyte injection or DNA recombination in embryonal stem (ES) cells allows mutations to be introduced into the germline. However, the earliest phenotype of the introduced...
Angrand, P O, Woodroofe, C P, Buchholz, F, Stewart, A F
When fused to the ligand binding domain (LBD) of steroid hormone nuclear receptors, site-specific recombinases (SSRs) acquire a ligand-dependent activity. Here, we describe the use of SSR-LBD fusion...
Rapid modification of bacterial artificial chromosomes by ET-recombination.
Muyrers, J P, Zhang, Y, Testa, G, Stewart, A F
We present a method to modify bacterial artificial chromosomes (BACs) resident in their host strain. The method is based on homologous recombination by ET-cloning. We have successfully modified BACs...
The chicken HPRT gene: a counter selectable marker for the DT40 cell line.
Fukagawa, T, Hayward, N, Yang, J, Azzalin, C, Griffin, D, Stewart, A F, ...
We describe the cloning, characterisation and chromosomal mapping of the chicken hprt gene together with the construction of two counter selectable hprt-/- DT40 derived cell lines. One of these cell...
Simplified generation of targeting constructs using ET recombination.
Angrand, P O, Daigle, N, Schöler, H R, Stewart, A F
ET recombination is a way to engineer DNA in Escherichia coli using homologous recombination. Here we develop the potential of ET recombination in two ways relevant to complex engineering exercises...
Mangin, M, Webb, A C, Dreyer, B E, Posillico, J T, Ikeda, K, Weir, E C, ...
Humoral hypercalcemia of malignancy is a common paraneoplastic syndrome that appears to be mediated in many instances by a parathyroid hormone-like peptide. Poly(A)+ RNA from a human renal carcinoma...
Soifer, N E, Van Why, S K, Ganz, M B, Kashgarian, M, Siegel, N J, Stewart, A F
Parathyroid hormone-related protein (PTHrP) is widely expressed in normal adult and fetal tissues, where it acts in an autocrine/paracrine fashion, stimulates growth and differentiation, and shares...
Insogna, K L, Stewart, A F, Morris, C A, Hough, L M, Milstone, L M, Centrella, M
A human parathyroid-like protein (PLP) has recently been isolated and cloned from human tumors associated with the paraneoplastic syndrome, humoral hypercalcemia of malignancy. PLP shares...
The chromo shadow domain, a second chromo domain in heterochromatin-binding protein 1, HP1.
The chromo domain was originally identified as a protein sequence motif common to the Drosophila chromatin proteins, Polycomb (Pc) and heterochromatin protein 1 [HP1; Paro and Hogness (1991) Proc....
Complete sequence of the bovine beta-lactoglobulin cDNA.
Alexander, L J, Hayes, G, Pearse, M J, Beattie, C W, Stewart, A F, Willis, I M, ...
Nucleotide sequences of bovine alpha S1- and kappa-casein cDNAs.
Stewart, A F, Willis, I M, Mackinlay, A G
The nucleotide sequences corresponding to bovine alpha S1- and kappa-casein mRNAs are presented. An unusual alpha S1-casein cDNA has been characterised whose 5' end commences upstream from its...
Stewart, A F, Mangin, M, Wu, T, Goumas, D, Insogna, K L, Burtis, W J, ...
Parathyroid hormone-like adenylate cyclase-stimulating proteins (hACSPs) have been implicated as one of the calcemic, bone-resorbing agents in patients with humoral hypercalcemia of malignancy. We...
Evolution of the casein multigene family: conserved sequences in the 5' flanking and exon regions.
Yu-Lee, L Y, Richter-Mann, L, Couch, C H, Stewart, A F, Mackinlay, A G, Rosen, J M
The rat alpha- and bovine alpha s1-casein genes have been isolated and their 5' sequences determined. The rat alpha-, beta-, gamma- and bovine alpha s1-casein genes contain similar 5' exon...
Nitsch, D, Stewart, A F, Boshart, M, Mestril, R, Weih, F, Schütz, G
The relationship between DNase I-hypersensitive sites (HSs) and transcriptional enhancers of the rat tyrosine aminotransferase (TAT) gene was examined by comparing HSs in and around the TAT gene with...
Rodan, S B, Insogna, K L, Vignery, A M, Stewart, A F, Broadus, A E, D'Souza, S M, ...
The culture media of three cell lines, a human prostate carcinoma (PC3), a rat Leydig cell tumor (Rice-500), and a rat carcinosarcoma (WRC-256), that were derived from tumors associated with humoral...
Stewart, A F, Insogna, K L, Goltzman, D, Broadus, A E
Humoral hypercalcemia of malignancy (HHM) most commonly results from secretion by tumors of an unidentified circulating calcemic factor that appears in clinical studies to stimulate both a...
Ikeda, K, Mangin, M, Dreyer, B E, Webb, A C, Posillico, J T, Stewart, A F, ...
The syndrome of humoral hypercalcemia of malignancy (HHM) appears to be mediated in many instances by a parathyroid hormone-like peptide, which has recently been purified, sequenced, and cloned....
Reik, A, Schütz, G, Stewart, A F
Induction of the rat tyrosine aminotransferase gene (TAT) with glucocorticoid hormones leads to formation of a nuclease hypersensitive site at the hormone-dependent enhancer located 2.5 kb upstream...
Nichols, M, Rientjes, J M, Stewart, A F
The estrogen receptor is capable of binding a diverse set of ligands that are broadly categorized as agonists or antagonists, depending on their abilities to induce or interfere with transcriptional...
Ringrose, L, Chabanis, S, Angrand, P O, Woodroofe, C, Stewart, A F
The probability that two sites on a linear DNA molecule will contact each other by looping depends on DNA flexibility. Although the flexibility of naked DNA in vitro is well characterized, looping in...