Dynamic Shear Tests of Soils for Seismic Analyses (9999)
Many excellent analytical procedures to evaluate the earthquake effects on soil deposits have been developed recently. In order to apply these analytical procedures to the designing of actual...
Nakajima, T, Tanaka, N, Sugiyama, E, Kamijo, Y, Hara, A, Hu, R, ...
Cloning and functional analysis of alkB genes in Alcanivorax borkumensis SK2 (2004)
Hara, A., Baik, S., Syutsubo, K., Misawa, N., Smits, T. H. M., Van Beilen, J. B., ...
Functional annotation of a full-length mouse cDNA collection (2001)
Kawai, J., Shinagawa, A., Shibata, K., Yoshino, M., Itoh, M., Ishii, Y., ...
The RIKEN Mouse Gene Encyclopaedia Project, a systematic approach to determining the full coding potential of the mouse genome, involves collection and sequencing of full-length complementary DNAs...
Tanaka, T, Makita, H, Kawabata, K, Mori, H, Kakumoto, M, Satoh, K, ...
Citrus auraptene inhibits chemically induced colonic aberrant crypt foci in male F344 rats (1997)
Tanaka, T, Kawabata, K, Kakumoto, M, Makita, H, Hara, A, Mori, H, ...
Fujii, K., Hirose, K., Hara, A., Shiraishi, M., Maruyama, T.
The relationship between ovarian development and serum vitellogenin levels was examined in the hybrid sturgeon, Huso huso x Acipenser ruthenus. Artificial spawning in the sturgeon was also done...
Suzuki, H, Yaoi, T, Kawai, J, Hara, A, Kuwajima, G, Wantanabe, S
We have developed a new method, designated restriction landmark cDNA scanning (RLCS), which displays many cDNA species quantitatively and simultaneously as two-dimensional gel spots. In this method...
Yutsudo, T, Okumura, K, Iwasaki, M, Hara, A, Kamitani, S, Minamide, W, ...
We recently cloned a gene encoding a new mitogenic factor (MF) from Streptococcus pyogenes NY-5. In the present study, we determined the distribution of this MF gene (mf) by PCR based upon its...
Nucleotide sequence of cDNA encoding the pregrowth hormone of red sea bream (Pagrus major).
Momota, H, Kosugi, R, Hiramatsu, H, Ohgai, H, Hara, A, Ishioka, H
Tsukada, Y, Hurwitz, E, Kashi, R, Sela, M, Hibi, N, Hara, A, ...
Monoclonal antibodies to rat alpha-fetoprotein (AFP) were produced by hybridization of mouse myeloma cells with spleen cells from mice immunized with rat AFP. The monoclonal antibodies as well as...
Deyashiki, Y, Taniguchi, H, Amano, T, Nakayama, T, Hara, A, Sawada, H
Two monomeric dihydrodiol dehydrogenases with pI values of 5.4 and 7.6 were co-purified with androsterone dehydrogenase activity to homogeneity from human liver. The two enzymes differed from each...
Ohmura, M, Hara, A, Nakagawa, M, Sawada, H
NAD(+)-linked and NADP(+)-linked 3 alpha-hydroxysteroid dehydrogenases were purified to homogeneity from hamster liver cytosol. The two monomeric enzymes, although having similar molecular masses of...
Hara, A, Harada, T, Nakagawa, M, Matsuura, K, Nakayama, T, Sawada, H
Dimeric and monomeric proteins containing dihydrodiol dehydrogenase and aldehyde reductase activities were purified from pig lens. The dimeric enzyme of Mr 65,000 specifically oxidized the...
Inazu, A, Sato, K, Nakayama, T, Deyashiki, Y, Hara, A, Nozawa, Y
Tetrahymena pyriformis was found to exhibit high NADPH-dependent 20-oxosteroid reductase activity that converted 17 alpha-hydroxyprogesterone into 17 alpha,20 alpha-dihydroxypregn-4-en-3-one. The...
Deyashiki, Y, Ogasawara, A, Nakayama, T, Nakanishi, M, Miyabe, Y, Sato, K, ...
Human liver contains two dihydrodiol dehydrogenases, DD2 and DD4, associated with 3 alpha-hydroxysteroid dehydrogenase activity. We have raised polyclonal antibodies that cross-reacted with the two...
Purification and characterization of two forms of microsomal carbonyl reductase in guinea pig liver.
Usui, S, Hara, A, Nakayama, T, Sawada, H
Two forms of microsomal carbonyl reductase, solubilized in Triton X-100, were purified to homogeneity from the liver of male guinea pigs, primarily by affinity, DEAE-Sephacel, gel-filtration and...
Hara, A, Hayashibara, M, Nakayama, T, Hasebe, K, Usui, S, Sawada, H
We have kinetically and immunologically demonstrated that testosterone 17 beta-dehydrogenase (NADP+) isoenzymes (EC 1.1.1.64) and aldehyde reductase (EC 1.1.1.2) from guinea-pig liver catalyse the...
Kinetic mechanism of pulmonary carbonyl reductase.
Matsuura, K, Nakayama, T, Nakagawa, M, Hara, A, Sawada, H
The kinetic mechanism of guinea-pig lung carbonyl reductase was studied at pH 7 in the forward reaction with five carbonyl substrates and NAD(P)H and in the reverse reaction with propan-2-ol and...
Distribution and characterization of dihydrodiol dehydrogenases in mammalian ocular tissues.
Hara, A, Nakayama, T, Harada, T, Kanazu, T, Shinoda, M, Deyashiki, Y, ...
The immunological relationship of two forms of dihydrodiol dehydrogenase (DD) in pig lens to pig muscle aldose reductase and kidney aldehyde reductase has been studied. Although the minor enzyme...
Inhibition of dimeric dihydrodiol dehydrogenases of rabbit and pig lens by ascorbic acid.
Hara, A, Shinoda, M, Kanazu, T, Nakayama, T, Deyashiki, Y, Sawada, H
The dehydrogenase activity of dimeric dihydrodiol dehydrogenases (DD) purified from pig and rabbit lenses was inhibited by either L-ascorbic acid or its epimer, isoascorbic acid, at pH 7.5....
Activation of human liver 3 alpha-hydroxysteroid dehydrogenase by sulphobromophthalein.
Matsuura, K, Tamada, Y, Deyashiki, Y, Miyabe, Y, Nakanishi, M, Ohya, I, ...
Human liver contains at least two isoenzymes (DD2 and DD4) of 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase. The NADP(H)-linked oxidoreductase activities of DD4 were activated more than 4-fold by...
Hara, A, Matsuura, K, Tamada, Y, Sato, K, Miyabe, Y, Deyashiki, Y, ...
We previously isolated three monomeric dihydrodiol dehydrogenases, DD1, DD2 and DD4, from human liver, and cloned a cDNA (C9) thought to encode DD2, which is identical with those for human...
Matsuura, K, Tamada, Y, Sato, K, Iwasa, H, Miwa, G, Deyashiki, Y, ...
A human liver 3 alpha-hydroxysteroid dehydrogenase isoenzyme, a member of the aldo-keto reductase family, shows a marked preference for NADP(H) over NAD(H), and is activated by sulphobromophthalein,...
Matsuura, K, Deyashiki, Y, Sato, K, Ishida, N, Miwa, G, Hara, A
Human liver dihydrodiol dehydrogenase isoenzymes (DD1 and DD2), in which only seven amino acid residues are substituted, differ remarkably in specificity for steroidal substrates and inhibitor...
Shiraishi, H, Ishikura, S, Matsuura, K, Deyashiki, Y, Ninomiya, M, Sakai, S, ...
Human liver contains three isoforms (DD1, DD2 and DD4) of dihydrodiol dehydrogenase with 20alpha- or 3alpha-hydroxysteroid dehydrogenase activity; the dehydrogenases belong to the aldo-oxo reductase...
Evidence for the presence of multiple forms of Sph kinase in human platelets.
Banno, Y, Kato, M, Hara, A, Nozawa, Y
The intracellular distribution of sphingosine (Sph) kinase activity was examined in human platelets. A large proportion (72%) of the total activity was found to be associated with the membrane...
Matsuura, K, Hara, A, Deyashiki, Y, Iwasa, H, Kume, T, Ishikura, S, ...
Human liver dihydrodiol dehydrogenase (DD; EC 1.3.1.20) exists in isoforms (DD1, DD2 and DD4) composed of 323 amino acids. DD1 and DD2 share 98% amino acid sequence identity, but show lower...
Cloning and sequencing of the cDNA species for mammalian dimeric dihydrodiol dehydrogenases.
Arimitsu, E, Aoki, S, Ishikura, S, Nakanishi, K, Matsuura, K, Hara, A
Cynomolgus and Japanese monkey kidneys, dog and pig livers and rabbit lens contain dimeric dihydrodiol dehydrogenase (EC 1.3.1.20) associated with high carbonyl reductase activity. Here we have...
Ohta, T, Ishikura, S, Shintani, S, Usami, N, Hara, A
Human dihydrodiol dehydrogenase with 3alpha-hydroxysteroid dehydrogenase activity exists in four forms (AKR1C1-1C4) that belong to the aldo-keto reductase (AKR) family. Recent crystallographic...
Ozeki, T, Takahashi, Y, Kume, T, Nakayama, K, Yokoi, T, Nunoya, K, ...
Human dihydrodiol dehydrogenase (DD) 4/aldo-keto reductase (AKR) 1C4 is a major isoform of hepatic DD that oxidizes trans-dihydrodiols of polycyclic aromatic hydrocarbons to reactive and redox-active...
Kawai, J, Hirose, K, Fushiki, S, Hirotsune, S, Ozawa, N, Hara, A, ...
Restriction landmark genomic scanning (RLGS) is a novel method which enables us to simultaneously visualize a large number of loci as two-dimensional gel spots. By this method, the status of DNA...
Kanazu, T, Shinoda, M, Nakayama, T, Deyashiki, Y, Hara, A, Sawada, H
3-Deoxyglucosone reductase activity in the extracts of rat, pig and human livers was potently inhibited by aldehyde reductase inhibitors. The major species of 3-deoxyglucosone reductase purified from...
Suzuki, H, Yaoi, T, Kawai, J, Hara, A, Kuwajima, G, Wantanabe, S
We have developed a new method, designated restriction landmark cDNA scanning (RLCS), which displays many cDNA species quantitatively and simultaneously as two-dimensional gel spots. In this method...
Yutsudo, T, Okumura, K, Iwasaki, M, Hara, A, Kamitani, S, Minamide, W, ...
We recently cloned a gene encoding a new mitogenic factor (MF) from Streptococcus pyogenes NY-5. In the present study, we determined the distribution of this MF gene (mf) by PCR based upon its...
Nucleotide sequence of cDNA encoding the pregrowth hormone of red sea bream (Pagrus major).
Momota, H, Kosugi, R, Hiramatsu, H, Ohgai, H, Hara, A, Ishioka, H
Tsukada, Y, Hurwitz, E, Kashi, R, Sela, M, Hibi, N, Hara, A, ...
Monoclonal antibodies to rat alpha-fetoprotein (AFP) were produced by hybridization of mouse myeloma cells with spleen cells from mice immunized with rat AFP. The monoclonal antibodies as well as...
Kawai, J, Hirose, K, Fushiki, S, Hirotsune, S, Ozawa, N, Hara, A, ...
Restriction landmark genomic scanning (RLGS) is a novel method which enables us to simultaneously visualize a large number of loci as two-dimensional gel spots. By this method, the status of DNA...
Deyashiki, Y, Taniguchi, H, Amano, T, Nakayama, T, Hara, A, Sawada, H
Two monomeric dihydrodiol dehydrogenases with pI values of 5.4 and 7.6 were co-purified with androsterone dehydrogenase activity to homogeneity from human liver. The two enzymes differed from each...
Ohmura, M, Hara, A, Nakagawa, M, Sawada, H
NAD(+)-linked and NADP(+)-linked 3 alpha-hydroxysteroid dehydrogenases were purified to homogeneity from hamster liver cytosol. The two monomeric enzymes, although having similar molecular masses of...
Hara, A, Harada, T, Nakagawa, M, Matsuura, K, Nakayama, T, Sawada, H
Dimeric and monomeric proteins containing dihydrodiol dehydrogenase and aldehyde reductase activities were purified from pig lens. The dimeric enzyme of Mr 65,000 specifically oxidized the...
Inazu, A, Sato, K, Nakayama, T, Deyashiki, Y, Hara, A, Nozawa, Y
Tetrahymena pyriformis was found to exhibit high NADPH-dependent 20-oxosteroid reductase activity that converted 17 alpha-hydroxyprogesterone into 17 alpha,20 alpha-dihydroxypregn-4-en-3-one. The...
Deyashiki, Y, Ogasawara, A, Nakayama, T, Nakanishi, M, Miyabe, Y, Sato, K, ...
Human liver contains two dihydrodiol dehydrogenases, DD2 and DD4, associated with 3 alpha-hydroxysteroid dehydrogenase activity. We have raised polyclonal antibodies that cross-reacted with the two...
Purification and characterization of two forms of microsomal carbonyl reductase in guinea pig liver.
Usui, S, Hara, A, Nakayama, T, Sawada, H
Two forms of microsomal carbonyl reductase, solubilized in Triton X-100, were purified to homogeneity from the liver of male guinea pigs, primarily by affinity, DEAE-Sephacel, gel-filtration and...
Hara, A, Hayashibara, M, Nakayama, T, Hasebe, K, Usui, S, Sawada, H
We have kinetically and immunologically demonstrated that testosterone 17 beta-dehydrogenase (NADP+) isoenzymes (EC 1.1.1.64) and aldehyde reductase (EC 1.1.1.2) from guinea-pig liver catalyse the...
Kinetic mechanism of pulmonary carbonyl reductase.
Matsuura, K, Nakayama, T, Nakagawa, M, Hara, A, Sawada, H
The kinetic mechanism of guinea-pig lung carbonyl reductase was studied at pH 7 in the forward reaction with five carbonyl substrates and NAD(P)H and in the reverse reaction with propan-2-ol and...
Distribution and characterization of dihydrodiol dehydrogenases in mammalian ocular tissues.
Hara, A, Nakayama, T, Harada, T, Kanazu, T, Shinoda, M, Deyashiki, Y, ...
The immunological relationship of two forms of dihydrodiol dehydrogenase (DD) in pig lens to pig muscle aldose reductase and kidney aldehyde reductase has been studied. Although the minor enzyme...
Inhibition of dimeric dihydrodiol dehydrogenases of rabbit and pig lens by ascorbic acid.
Hara, A, Shinoda, M, Kanazu, T, Nakayama, T, Deyashiki, Y, Sawada, H
The dehydrogenase activity of dimeric dihydrodiol dehydrogenases (DD) purified from pig and rabbit lenses was inhibited by either L-ascorbic acid or its epimer, isoascorbic acid, at pH 7.5....
Kanazu, T, Shinoda, M, Nakayama, T, Deyashiki, Y, Hara, A, Sawada, H
3-Deoxyglucosone reductase activity in the extracts of rat, pig and human livers was potently inhibited by aldehyde reductase inhibitors. The major species of 3-deoxyglucosone reductase purified from...
Activation of human liver 3 alpha-hydroxysteroid dehydrogenase by sulphobromophthalein.
Matsuura, K, Tamada, Y, Deyashiki, Y, Miyabe, Y, Nakanishi, M, Ohya, I, ...
Human liver contains at least two isoenzymes (DD2 and DD4) of 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase. The NADP(H)-linked oxidoreductase activities of DD4 were activated more than 4-fold by...
Hara, A, Matsuura, K, Tamada, Y, Sato, K, Miyabe, Y, Deyashiki, Y, ...
We previously isolated three monomeric dihydrodiol dehydrogenases, DD1, DD2 and DD4, from human liver, and cloned a cDNA (C9) thought to encode DD2, which is identical with those for human...
Matsuura, K, Tamada, Y, Sato, K, Iwasa, H, Miwa, G, Deyashiki, Y, ...
A human liver 3 alpha-hydroxysteroid dehydrogenase isoenzyme, a member of the aldo-keto reductase family, shows a marked preference for NADP(H) over NAD(H), and is activated by sulphobromophthalein,...
Matsuura, K, Deyashiki, Y, Sato, K, Ishida, N, Miwa, G, Hara, A
Human liver dihydrodiol dehydrogenase isoenzymes (DD1 and DD2), in which only seven amino acid residues are substituted, differ remarkably in specificity for steroidal substrates and inhibitor...
Shiraishi, H, Ishikura, S, Matsuura, K, Deyashiki, Y, Ninomiya, M, Sakai, S, ...
Human liver contains three isoforms (DD1, DD2 and DD4) of dihydrodiol dehydrogenase with 20alpha- or 3alpha-hydroxysteroid dehydrogenase activity; the dehydrogenases belong to the aldo-oxo reductase...
Evidence for the presence of multiple forms of Sph kinase in human platelets.
Banno, Y, Kato, M, Hara, A, Nozawa, Y
The intracellular distribution of sphingosine (Sph) kinase activity was examined in human platelets. A large proportion (72%) of the total activity was found to be associated with the membrane...
Matsuura, K, Hara, A, Deyashiki, Y, Iwasa, H, Kume, T, Ishikura, S, ...
Human liver dihydrodiol dehydrogenase (DD; EC 1.3.1.20) exists in isoforms (DD1, DD2 and DD4) composed of 323 amino acids. DD1 and DD2 share 98% amino acid sequence identity, but show lower...
Cloning and sequencing of the cDNA species for mammalian dimeric dihydrodiol dehydrogenases.
Arimitsu, E, Aoki, S, Ishikura, S, Nakanishi, K, Matsuura, K, Hara, A
Cynomolgus and Japanese monkey kidneys, dog and pig livers and rabbit lens contain dimeric dihydrodiol dehydrogenase (EC 1.3.1.20) associated with high carbonyl reductase activity. Here we have...
Ohta, T, Ishikura, S, Shintani, S, Usami, N, Hara, A
Human dihydrodiol dehydrogenase with 3alpha-hydroxysteroid dehydrogenase activity exists in four forms (AKR1C1-1C4) that belong to the aldo-keto reductase (AKR) family. Recent crystallographic...
Ozeki, T, Takahashi, Y, Kume, T, Nakayama, K, Yokoi, T, Nunoya, K, ...
Human dihydrodiol dehydrogenase (DD) 4/aldo-keto reductase (AKR) 1C4 is a major isoform of hepatic DD that oxidizes trans-dihydrodiols of polycyclic aromatic hydrocarbons to reactive and redox-active...
Physical biochemical properties of IgM from a teleost fish.
Mochida, K, Lou, Y H, Hara, A, Yamauchi, K
Teleost fish are able to produce IgM class antibody, as are other vertebrates. When the teleost fish Oreochromis niloticus was immunized with bovine serum albumin (BSA), it produced antibody to BSA...
Yamaguchi, N, Shimizu, S, Hara, A, Saito, T
Pregnant mice were stimulated by sheep erythrocytes (SRBC) and the active immune responses of their offspring were investigated. The offspring whose mothers were stimulated with SRBC did not develop...
Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs
Okazaki, Y, Furuno, M, Kasukawa, T, Adachi, J, Bono, H, Kondo, S, ...
Only a small proportion of the mouse genome is transcribed into mature messenger RNA transcripts. There is an international collaborative effort to identify all full-length mRNA transcripts from the...
Hibi, T, Ohara, M, Toda, K, Hara, A, Ogata, H, Iwao, Y, ...
Serum anticolon antibody and in vitro anti-colon antibody production by peripheral blood and mucosal lymphocytes was investigated in patients with ulcerative colitis. The frequency of serum anticolon...
Loss of DCC expression in astrocytomas: relation to p53 abnormalities, cell kinetics, and survival
Hara, A, Saegusa, M, Mikami, T, Okayasu, I
Aims—Although frequent reduction or loss of DCC (deleted in colorectal carcinomas) has been demonstrated in gliomas, the association with cell kinetics and survival is still unclear.
Kato, K, Long, N K, Makita, H, Toida, M, Yamashita, T, Hatakeyama, D, ...
RECK is a novel tumour suppressor gene that negatively regulates matrix metalloproteinases (MMPs) and inhibits tumour invasion, angiogenesis and metastasis. In the present study, we investigated the...
Hara, A, Yoshimi, N, Yamada, Y, Matsunaga, K, Kawabata, K, Sugie, S, ...
The present study was designed to investigate the effect of Fas-mediated liver cell apoptosis, induced by a hamster monoclonal antibody against mouse Fas antigen, on diethylnitrosamine (DEN)-induced...