Schuler, T.H., Clark, A.J., Clark, S.J., Poppy, G.M., Stewart, C.N., Denholm, I.
Crops transformed to express Bacillus thuringiensis (Bt) toxins can cause close to 100% mortality of certain target pest species. This study assessed the effect of target pest reduction on the...
Casjens, S., Winn-Stapley, D. A., Gilcrease, E. B., Morona, Renato, Kuhlewein, C., Chua, J. E.H., ...
Copyright © 2004 Elsevier Ltd. All rights reserved.
Casjens, S., Winn-Stapley, D. A., Gilcrease, E. B., Morona, Renato, Kuhlewein, C., Chua, J. E.H., ...
Copyright © 2004 Elsevier Ltd. All rights reserved.
Schuler, T.H., Potting, R.P.J., Denholm, I., Clark, S.J., Clark, A.J., Stewart, C.N., ...
Parasitoids are important natural enemies of many pest species and are used extensively in biological and integrated control programmes. Crop plants transformed to express toxin genes derived from...
Schuler, T.H., Potting, R.P.J., Denholm, I., Clark, S.J., Clark, A.J., Stewart, C.N., ...
Parasitoids are important natural enemies of many pest species and are used extensively in biological and integrated control programmes. Crop plants transformed to express toxin genes derived from...
Feelders, R.A., Herder, W.W. De, Lamberts, S.W., Hofland, L.J., Koetsveld, P.M. Van, Verhoef-Post, M., ...
In a substantial part of adrenal adenomas and hyperplasias from patients with Cushing's syndrome, cortisol production is controlled by the expression of aberrant hormone receptors on adrenocortical...
Gene association studies in small for gestational age infants (2002)
Johnston, L.B., Arends N, Dahlgren J, Leger J, Czernichow P, Albertsson-Wikland K, ...
Analysis of Supply Support Impacts on Equipment Operational Availability. (2002)
Clark,A. J., Clark,W. E., Eaton,T. E., Reynolds,A. J.
Research was conducted to determine the impact, on material condition of ships' system and equipments, of three parameters; reliability, maintainability, and supply response. A computer program was...
THE LOGISTICS COMPOSITE MODEL: AN OVERALL VIEW (2002)
Fisher, R. R., Drake, W. W., Delfausse, J. J., Clark, A. J., Buchanan, A. L.
A description of the Logistics Composite Model (L-COM), a computer model developed to simulate the overall operations and support functions at an Air Force base. L-COM consists of three main...
McClenaghan, M., Hitchin, E., Stevenson, E.M., Clark, A.J., Holt, C., Leaver, J.
We have shown that the cellular mechanisms of the mammary gland can be used to produce a phosphorylated form of a normally unphosphorylated milk protein. This was achieved by the insertion of a...
Optimisation heuristics for cryptology (1998)
Presented to the Information Security Research Centre, Queensland University of Technology. Includes bibliographical references (p. 135-142)
Complete nucleotide sequence of the genomic ovine {beta}-lactoglobulin gene (1988)
Harris, S., Ali, S., Anderson, S., Archibald, A. L., Clark, A. J.
The fine structure and evolution of human repeated DNA. (1982)
Thesis (Ph. D.)--University of Edinburgh, 1982.
Photosynthetic capabilities of cultured plant cells. (1981)
Thesis (Ph. D.)--University of Sheffield, 1981.
Archaeological detection by resistivity. (1980)
Thesis (Ph. D.)--University of Southampton, 1980.
Dobie, K W, Lee, M, Fantes, J A, Graham, E, Clark, A J, Springbett, A, ...
Mice carrying an ovine beta-lactoglobulin (BLG) transgene secrete BLG protein into their milk. To explore transgene expression stability, we studied expression levels in three BLG transgenic mouse...
Yull, F, Harold, G, Wallace, R, Cowper, A, Percy, J, Cottingham, I, ...
Transgenic mice and sheep secrete only low levels of human factor IX in their milk because of an aberrant splicing of the transgene RNA in the mammary gland. Removal of the cryptic 3' splice site...
Archibald, A L, McClenaghan, M, Hornsey, V, Simons, J P, Clark, A J
Reduced circulating levels of alpha 1-antitrypsin (alpha 1 AT) are associated with certain alpha 1 AT genotypes and increased susceptibility to emphysema. Unfortunately, the amounts of alpha 1 AT...
Sandler, S J, Satin, L H, Samra, H S, Clark, A J
The process of homologous recombination has been documented in bacterial and eucaryotic organisms. The Escherichia coli RecA and Saccharomyces cerevisiae Rad51 proteins are the archetypal members of...
Mammalian cDNA and prokaryotic reporter sequences silence adjacent transgenes in transgenic mice.
Clark, A J, Harold, G, Yull, F E
The ovine beta-lactoglobulin gene is expressed efficiently and at high levels in the mammary gland of transgenic mice. In contrast, when this gene is linked to a second gene construct comprising a...
Linkage of the angiotensinogen gene locus to human essential hypertension in African Caribbeans.
Caulfield, M, Lavender, P, Newell-Price, J, Farrall, M, Kamdar, S, Daniel, H, ...
The renin-angiotensin system regulates blood pressure and sodium balance. The angiotensinogen gene which encodes the key substrate within this system has been linked to essential hypertension in...
Transcription of the Escherichia coli recE gene from a promoter in Tn5 and IS50.
Clark, A J, Satin, L, Chu, C C
Six sbc::Tn5 insertions and one sbc::IS50 insertion, which cause recE expression in Escherichia coli, have been cloned, and their DNA sequences have been determined. The sites of insertion are found...
RecOR suppression of recF mutant phenotypes in Escherichia coli K-12.
The recF, recO, and recR genes form the recFOR epistasis group for DNA repair. recF mutants are sensitive to UV irradiation and fail to properly induce the SOS response. Using plasmid derivatives...
Mutational analysis of sequences in the recF gene of Escherichia coli K-12 that affect expression.
The level of translation of recF-lacZ fusions is reduced 20-fold by nucleotides 49 to 146 of recF. In this region of recF, we found a previously described ribosome-interactive sequence called epsilon...
Clark, A J, Sharma, V, Brenowitz, S, Chu, C C, Sandler, S, Satin, L, ...
The nucleotide sequence of the C-terminal region of the recE gene of the Rac prophage of Escherichia coli K-12 reveals the presence of a partially overlapping reading frame we call recT. Deletion...
Evidence for ATP binding and double-stranded DNA binding by Escherichia coli RecF protein.
RecF protein is one of the important proteins involved in DNA recombination and repair. RecF protein has been shown to bind single-stranded DNA (ssDNA) in the absence of ATP (T. J. Griffin IV and R....
Chu, C C, Templin, A, Clark, A J
The nucleotide sequences of a small gene, racC, and the adjacent N-terminal half of the wild-type recE gene are presented. A frameshift mutation, recE939, inactivating recE and preventing synthesis...
Luisi-DeLuca, C, Clark, A J, Kolodner, R D
Exonuclease VIII (exoVIII) of Escherichia coli has been purified from a strain carrying a plasmid-encoded recE gene by using a new procedure. This procedure yielded 30 times more protein per gram of...
Synthesis of linear plasmid multimers in Escherichia coli K-12.
Linear plasmid multimers were identified in extracts of recB21 recC22 strains containing derivatives of the ColE1-type plasmids pACYC184 and pBR322. A mutation in sbcB increases the proportion of...
Two-component suppression of recF143 by recA441 in Escherichia coli K-12.
Volkert, M R, Margossian, L J, Clark, A J
Sensitivity to UV irradiation conferred by recF143 was partially suppressed by recA441 (also known as tif-1). A temperature-conditional component depended on uvrA function and is thought to involve...
Genetic analysis of the recJ gene of Escherichia coli K-12.
An insertion of transposon Tn10 was isolated in a gene essential for the RecF pathway of recombination. This mutation and six other previously isolated rec mutations, including one in the recJ gene,...
Willis, D K, Satin, L H, Clark, A J
Using pBR322 as a vector, we cloned a 5.95-kilobase fragment of the Rac prophage together with 1.70 kilobases of a flanking Escherichia coli chromosome sequence. The resulting plasmid (pRAC1) was...
Overproduction of the Escherichia coli recA protein without stimulation of its proteolytic activity.
Plasmid pBEU14, which carries the Escherichia coli recA+ gene and which can be amplified by manipulation of growth temperature, was constructed. When pBEU14 deoxyribonucleic acid was amplified, a...
Genetic analysis of the RecE pathway of genetic recombination in Escherichia coli K-12.
Gillen, J R, Willis, D K, Clark, A J
The RecE pathway of genetic recombination in Escherichia coli K-12 was defined to be the pathway that is utilized in deoxyribonucleic acid exonuclease V (ExoV)-defective cells which express...
Cloning of the Escherichia coli recJ chromosomal region and identification of its encoded proteins.
A 9.6-kilobase BamHI-SalI fragment carrying recJ+ was cloned into vector pBR322. Deletion and transposon mutagenesis were used to map the recJ gene on this fragment. The maxicell protein-labeling...
Assignment of tra cistrons to EcoRI fragments of F sex factor DNA.
Achtman, M, Skurray, R A, Thompson, R, Helmuth, R, Hall, S, Beutin, L, ...
We describe here the cloning of single EcoRI fragments from the tra region of F DNA using ColE1::Tn3 as vector. These plasmids, as well as the series of Skurray et al. (Proc. Natl. Acad. Sci. U.S.A....
Suppressibility of recA, recB, and recC mutations by nonsense suppressors.
Templin, A, Margossian, L, Clark, A J
Mutations in the recA, recB, and recC genes of Escherichia coli K-12 were surveyed to ascertain whether or not they are suppressed by nonsense suppressors. Several mutations which map in or near the...
Purification and properties of two deoxyribonucleases of Pseudomonas aeruginosa.
A survey of the major deoxyribonucleases in Pseudomonas aeruginosa strain PAO was undertaken. Two activities predominated in Brij-58 lysates of this organism. These have been purified from...
cis-Dominant, transfer-deficient mutants of the Escherichia coli K-12 F sex factor.
Rare conjugational progeny formed by crossing each of five Hfr strains with a recA-F- strain have been characterized. Selection was made for a proximal Hfr marker, taking strict precautions to...
Heteroduplex analysis of tra delta f' plasmids and the mechanism of their formation.
Guyer, M S, Davidson, N, Clark, A J
Four tra delta FargG+ plasmids, derived from matings between Hfr AB312 and a recA recipient, have been shown to have deletions of at least 50% of the F genome, including the region in which the tra...
Ohnishi, Y, Iguma, H, Ono, T, Nagaishi, H, Clark, A J
F+ Escherichi coli cells that contain an srnA mutant allele degrade their stable ribonucleic acid (RNA) extensively after RNA synthesis is blocked at 42 degrees C. The relevant gene promoting...
Madiraju, M V, Templin, A, Clark, A J
A mutation partially suppressing the UV sensitivity caused by recF143 in a uvrA6 background was located at codon 37 of recA where GTG (valine) became ATG (methionine). This mutation, originally named...
Transposon-mediated conjugational transmission of nonconjugative plasmids.
Crisona, N J, Nowak, J A, Nagaishi, H, Clark, A J
When coresident with conjugative plasmid pNC21, the nonconjugative deletion F-prime pJC59, which retains the F transfer origin oriT, was transmitted to transconjugants at a frequency comparable to...
Ray, D W, Littlewood, A C, Clark, A J, Davis, J R, White, A
Some human small cell lung carcinomas (SCLC) secrete proopiomelanocortin (POMC) derived peptides, but in contrast to the pituitary, glucocorticoids fail to inhibit this hormone production. We have...
Strain JC10240 (Hfr PO45 srlC300::Tn10 recA56 thr-300 ilv-318 rpsE300) was constructed. On account of the close linkage of Tn10 to recA56, the latter can be moved to other Escherichia coli (and...
Buzzetti, R, McLoughlin, L, Lavender, P M, Clark, A J, Rees, L H
Using Northern blotting with a human genomic DNA probe for the pro-opiomelanocortin (POMC) gene, we have shown specific mRNA in normal human peripheral mononuclear cells (PBMC); the presence of...
Sandler, S J, Chackerian, B, Li, J T, Clark, A J
We have compared the recF genes from Escherichia coli K-12, Salmonella typhimurium, Pseudomonas putida, and Bacillus subtilis at the DNA and amino acid sequence levels. To do this we determined the...
Characteristics of Some Multiply Recombination-Deficient Strains of Escherichia coli
Strains of Escherichia coli have been made carrying lesions in more than one gene determining recombination. The following genotypes were constructed and verified: recC22 recB21 recA+, recC22 recB21...
Evidence that rnmB is the operator of the Escherichia coli recA gene.
Volkert, M R, Margossian, L J, Clark, A J
rnmB281 leads to high constitutive levels of recA protein such that no increase after UV-inducing treatment occurs. The mutation maps in or near the portion of recA corresponding to the NH2-terminal...
Effect of RecF protein on reactions catalyzed by RecA protein.
RecF protein is one of at least three single strand DNA (ssDNA) binding proteins which act in recombination and repair in Escherichia coli. In this paper we show that our RecF protein preparation...
Watson, C J, Gordon, K E, Robertson, M, Clark, A J
The minimal 5' regulatory region of the sheep beta-lactoglobulin gene (BLG), as defined in transgenic mice, was used to identify nuclear factors which may be involved in milk protein gene expression...
Farrell, W E, Clark, A J, Stewart, M F, Crosby, S R, White, A
We have previously reported that a human small cell lung cancer (SCLC) cell line (COR L103) that expresses the proopiomelanocortin (POMC) gene and secretes ACTH precursor peptides is relatively...
Molecular cloning of DNA from F sex factor of Escherichia coli K-12.
Skurray, R A, Nagaishi, H, Clark, A J
We describe the molecular cloning of various DNA segments generated by partial EcoRI endonuclease digestion of the sex factor F. These segments have been analyzed by agarose gel electrophoresis of...
Sequence-specific recombination of plasmid ColE1.
The conjugal transmission of plasmid ColE1 and its derivatives can lead to recA-independent recombination. The recombination is not observed during vegetative growth and takes place specifically...
The 45-kb unit of major urinary protein gene organization is a gigantic imperfect palindrome.
Bishop, J O, Selman, G G, Hickman, J, Black, L, Saunders, R D, Clark, A J
The multigene family which codes for the mouse major urinary proteins consists of about 35 genes. Most of these are members of two distinct groups, group 1 and group 2. The group 1 and group 2 genes...
Merlino, G T, Ishii, S, Whang-Peng, J, Knutsen, T, Xu, Y H, Clark, A J, ...
A431 cells have an amplification of the epidermal growth factor (EGF) receptor gene, the cellular homolog of the v-erb B oncogene, and overproduce an aberrant 2.9-kilobase RNA that encodes a portion...
Epidermal growth factor regulates the expression of its own receptor.
Clark, A J, Ishii, S, Richert, N, Merlino, G T, Pastan, I
The epidermal growth factor (EGF) receptor gene is the cellular homolog of the avian erythroblastosis virus erbB oncogene. Control of EGF receptor expression determines cellular responsiveness to EGF...
Molecular analysis of the recF gene of Escherichia coli.
Blanar, M A, Sandler, S J, Armengod, M E, Ream, L W, Clark, A J
We analyzed the nucleotide sequence of a 1.325-kilobase region of wild-type Escherichia coli containing a functional recF gene and six Tn3 mutations that inactivate recF. The analysis shows a...
Evolutionary amplification of a pseudogene.
Ghazal, P, Clark, A J, Bishop, J O
The family of mouse major urinary protein (MUP) genes has about 35 members, clustered together on chromosome 4. Most of the genes belong to two major subfamilies (group 1 and group 2) each with 12-15...
Merlino, G T, Xu, Y H, Richert, N, Clark, A J, Ishii, S, Banks-Schlegel, S, ...
The human epidermal growth factor (EGF) receptor is known to be homologous to the v-erb B oncogene protein of the avian erythroblastosis virus. Overexpression of the EGF receptor gene in A431...
Isolation of Exonuclease VIII: The Enzyme Associated with the sbcA Indirect Suppressor
Kushner, Sidney R., Nagaishi, Haruko, Clark, A. J.
recB and/or recC deficiency in Escherichia coli K-12 is indirectly suppressed by the presence of sbcA- mutations. sbcA- strains contain an increased level of an ATP-independent nuclease. Genetic and...
Two main groups of mouse major urinary protein genes, both largely located on chromosome 4.
Bishop, J O, Clark, A J, Clissold, P M, Hainey, S, Francke, U
Fourteen different major urinary protein (MUP) genomic clones from BALB/c mice were isolated. By restriction site mapping, six of these form two sets of three overlapping clones. By the criterion of...
Sequence structures of a mouse major urinary protein gene and pseudogene compared.
Clark, A J, Ghazal, P, Bingham, R W, Barrett, D, Bishop, J O
Laboratory mouse strains carry approximately 35 major urinary protein (MUP) genes per haploid genome, tightly clustered together on chromosome 4. Most belong to two main groups (Groups 1 and 2). The...
Clark, A J, Chave-Cox, A, Ma, X, Bishop, J O
Here we compare the exonic sequences of four Group 1 mouse major urinary protein (MUP) genes and four Group 1 cDNA sequences. These define seven different nucleotide sequences which differ from each...
Clark, A J, Clissold, P M, Al Shawi, R, Beattie, P, Bishop, J
The multigene family which codes for the mouse major urinary proteins (MUPs) consists of approximately 35 genes. Most of these are members of two different groups, Group 1 and Group 2, which can be...
Clark, A J, Hickman, J, Bishop, J
The multigene family which codes for the mouse major urinary proteins (MUPs) consists of approximately 35 genes. Most of these are members of two different groups, Group 1 and Group 2, which can be...
Wilde, C J, Clark, A J, Kerr, M A, Knight, C H, McClenaghan, M, Simons, J P
Mammary development and milk secretion were studied in transgenic mice which exhibited mammary tissue-specific expression of the sheep beta-lactoglobulin gene, and secreted significant quantities of...
Position-independent expression of the ovine beta-lactoglobulin gene in transgenic mice.
Whitelaw, C B, Harris, S, McClenaghan, M, Simons, J P, Clark, A J
The major milk whey protein of sheep, beta-lactoglobulin (BLG), is expressed specifically in the mammary gland in a developmentally regulated pattern. To identify the cis-acting DNA regions involved...
Secretory proteins compete for production in the mammary gland of transgenic mice.
McClenaghan, M, Springbett, A, Wallace, R M, Wilde, C J, Clark, A J
To explore the possibility that genes might compete for expression, we have studied transgenic mice producing high levels of the sheep milk protein, beta-lactoglobulin (BLG), in the mammary gland....
1. The membrane potential in intact cells of Rhodopseudomonas capsulata during photosynthesis and during dark respiration has been measured by two independent methods. 2. The light-induced and...
Fouts, K E, Wasie-Gilbert, T, Willis, D K, Clark, A J, Barbour, S D
Fourteen mitomycin-resistant revertants of a recB21 recC22 strain were isolated after Tn5 mutagenesis. Eight of the mutations (type I) were essentially inseparable from aphA+ (Kanr) of Tn5; six (type...
Willis, D K, Fouts, K E, Barbour, S D, Clark, A J
Fourteen Tn5-generated mutations of the Rac prophage, called sbc because they suppress recB21 recC22, were found to fall into two distinct types: type I mutations, which were insertions of Tn5, and...
Mahajan, S. K., Chu, C. C., Willis, D. K., Templin, A., Clark, A. J.
We have mapped the extents of two deletion sbcA mutations which result in production of DNA exonuclease VIII (ExoVIII). One mutation, sbcA8, deletes about 140 kb of DNA which includes most of the Rac...
We showed that sufficient overexpression of the wild-type recF gene interfered with three normal cell functions: (1) UV induction of transcription from the LexA-protein-repressed sulA promoter, (2)...
Sandler, S. J., Samra, H. S., Clark, A. J.
First identified as an essential component of the φX174 in vitro DNA replication system, PriA has ATPase, helicase, translocase, and primosome-assembly activities. priA1::kan strains of Escherichia...
A Proposal for a Uniform Nomenclature in Bacterial Genetics
Demerec, M., Adelberg, E. A., Clark, A. J., Hartman, Philip E.
Genetic Analysis of a Double Male Strain of ESCHERICHIA COLI K12
Falkinham, Joseph O., Clark, A. J.
The behavior of a double male strain of Escherichia coli K12 has been compared to that of its parents and a primary F' strain carrying F14 in order to determine whether the genome of the double male,...
Diabetic impotence: studies of nocturnal erection during REM sleep.
Hosking, D J, Bennet, T, Hampton, J R, Evans, D F, Clark, A J, Robertson, G
Nocturnal erections were studied in 30 diabetic patients who complained of impotence and in 11 healthy volunteers. The maximum increase in penile circumference was measured by a penile strain-gauge...
Dobie, K W, Lee, M, Fantes, J A, Graham, E, Clark, A J, Springbett, A, ...
Mice carrying an ovine beta-lactoglobulin (BLG) transgene secrete BLG protein into their milk. To explore transgene expression stability, we studied expression levels in three BLG transgenic mouse...
Yull, F, Harold, G, Wallace, R, Cowper, A, Percy, J, Cottingham, I, ...
Transgenic mice and sheep secrete only low levels of human factor IX in their milk because of an aberrant splicing of the transgene RNA in the mammary gland. Removal of the cryptic 3' splice site...
Archibald, A L, McClenaghan, M, Hornsey, V, Simons, J P, Clark, A J
Reduced circulating levels of alpha 1-antitrypsin (alpha 1 AT) are associated with certain alpha 1 AT genotypes and increased susceptibility to emphysema. Unfortunately, the amounts of alpha 1 AT...
Sandler, S J, Satin, L H, Samra, H S, Clark, A J
The process of homologous recombination has been documented in bacterial and eucaryotic organisms. The Escherichia coli RecA and Saccharomyces cerevisiae Rad51 proteins are the archetypal members of...
Mammalian cDNA and prokaryotic reporter sequences silence adjacent transgenes in transgenic mice.
Clark, A J, Harold, G, Yull, F E
The ovine beta-lactoglobulin gene is expressed efficiently and at high levels in the mammary gland of transgenic mice. In contrast, when this gene is linked to a second gene construct comprising a...
Linkage of the angiotensinogen gene locus to human essential hypertension in African Caribbeans.
Caulfield, M, Lavender, P, Newell-Price, J, Farrall, M, Kamdar, S, Daniel, H, ...
The renin-angiotensin system regulates blood pressure and sodium balance. The angiotensinogen gene which encodes the key substrate within this system has been linked to essential hypertension in...
Transcription of the Escherichia coli recE gene from a promoter in Tn5 and IS50.
Clark, A J, Satin, L, Chu, C C
Six sbc::Tn5 insertions and one sbc::IS50 insertion, which cause recE expression in Escherichia coli, have been cloned, and their DNA sequences have been determined. The sites of insertion are found...
RecOR suppression of recF mutant phenotypes in Escherichia coli K-12.
The recF, recO, and recR genes form the recFOR epistasis group for DNA repair. recF mutants are sensitive to UV irradiation and fail to properly induce the SOS response. Using plasmid derivatives...
Mutational analysis of sequences in the recF gene of Escherichia coli K-12 that affect expression.
The level of translation of recF-lacZ fusions is reduced 20-fold by nucleotides 49 to 146 of recF. In this region of recF, we found a previously described ribosome-interactive sequence called epsilon...
Clark, A J, Sharma, V, Brenowitz, S, Chu, C C, Sandler, S, Satin, L, ...
The nucleotide sequence of the C-terminal region of the recE gene of the Rac prophage of Escherichia coli K-12 reveals the presence of a partially overlapping reading frame we call recT. Deletion...
Evidence for ATP binding and double-stranded DNA binding by Escherichia coli RecF protein.
RecF protein is one of the important proteins involved in DNA recombination and repair. RecF protein has been shown to bind single-stranded DNA (ssDNA) in the absence of ATP (T. J. Griffin IV and R....
Chu, C C, Templin, A, Clark, A J
The nucleotide sequences of a small gene, racC, and the adjacent N-terminal half of the wild-type recE gene are presented. A frameshift mutation, recE939, inactivating recE and preventing synthesis...
Luisi-DeLuca, C, Clark, A J, Kolodner, R D
Exonuclease VIII (exoVIII) of Escherichia coli has been purified from a strain carrying a plasmid-encoded recE gene by using a new procedure. This procedure yielded 30 times more protein per gram of...
Synthesis of linear plasmid multimers in Escherichia coli K-12.
Linear plasmid multimers were identified in extracts of recB21 recC22 strains containing derivatives of the ColE1-type plasmids pACYC184 and pBR322. A mutation in sbcB increases the proportion of...
Two-component suppression of recF143 by recA441 in Escherichia coli K-12.
Volkert, M R, Margossian, L J, Clark, A J
Sensitivity to UV irradiation conferred by recF143 was partially suppressed by recA441 (also known as tif-1). A temperature-conditional component depended on uvrA function and is thought to involve...
Genetic analysis of the recJ gene of Escherichia coli K-12.
An insertion of transposon Tn10 was isolated in a gene essential for the RecF pathway of recombination. This mutation and six other previously isolated rec mutations, including one in the recJ gene,...
Willis, D K, Satin, L H, Clark, A J
Using pBR322 as a vector, we cloned a 5.95-kilobase fragment of the Rac prophage together with 1.70 kilobases of a flanking Escherichia coli chromosome sequence. The resulting plasmid (pRAC1) was...
Overproduction of the Escherichia coli recA protein without stimulation of its proteolytic activity.
Plasmid pBEU14, which carries the Escherichia coli recA+ gene and which can be amplified by manipulation of growth temperature, was constructed. When pBEU14 deoxyribonucleic acid was amplified, a...
Genetic analysis of the RecE pathway of genetic recombination in Escherichia coli K-12.
Gillen, J R, Willis, D K, Clark, A J
The RecE pathway of genetic recombination in Escherichia coli K-12 was defined to be the pathway that is utilized in deoxyribonucleic acid exonuclease V (ExoV)-defective cells which express...
Fouts, K E, Wasie-Gilbert, T, Willis, D K, Clark, A J, Barbour, S D
Fourteen mitomycin-resistant revertants of a recB21 recC22 strain were isolated after Tn5 mutagenesis. Eight of the mutations (type I) were essentially inseparable from aphA+ (Kanr) of Tn5; six (type...
Willis, D K, Fouts, K E, Barbour, S D, Clark, A J
Fourteen Tn5-generated mutations of the Rac prophage, called sbc because they suppress recB21 recC22, were found to fall into two distinct types: type I mutations, which were insertions of Tn5, and...
Cloning of the Escherichia coli recJ chromosomal region and identification of its encoded proteins.
A 9.6-kilobase BamHI-SalI fragment carrying recJ+ was cloned into vector pBR322. Deletion and transposon mutagenesis were used to map the recJ gene on this fragment. The maxicell protein-labeling...
Assignment of tra cistrons to EcoRI fragments of F sex factor DNA.
Achtman, M, Skurray, R A, Thompson, R, Helmuth, R, Hall, S, Beutin, L, ...
We describe here the cloning of single EcoRI fragments from the tra region of F DNA using ColE1::Tn3 as vector. These plasmids, as well as the series of Skurray et al. (Proc. Natl. Acad. Sci. U.S.A....
Suppressibility of recA, recB, and recC mutations by nonsense suppressors.
Templin, A, Margossian, L, Clark, A J
Mutations in the recA, recB, and recC genes of Escherichia coli K-12 were surveyed to ascertain whether or not they are suppressed by nonsense suppressors. Several mutations which map in or near the...
Purification and properties of two deoxyribonucleases of Pseudomonas aeruginosa.
A survey of the major deoxyribonucleases in Pseudomonas aeruginosa strain PAO was undertaken. Two activities predominated in Brij-58 lysates of this organism. These have been purified from...
cis-Dominant, transfer-deficient mutants of the Escherichia coli K-12 F sex factor.
Rare conjugational progeny formed by crossing each of five Hfr strains with a recA-F- strain have been characterized. Selection was made for a proximal Hfr marker, taking strict precautions to...
Heteroduplex analysis of tra delta f' plasmids and the mechanism of their formation.
Guyer, M S, Davidson, N, Clark, A J
Four tra delta FargG+ plasmids, derived from matings between Hfr AB312 and a recA recipient, have been shown to have deletions of at least 50% of the F genome, including the region in which the tra...
Ohnishi, Y, Iguma, H, Ono, T, Nagaishi, H, Clark, A J
F+ Escherichi coli cells that contain an srnA mutant allele degrade their stable ribonucleic acid (RNA) extensively after RNA synthesis is blocked at 42 degrees C. The relevant gene promoting...
Madiraju, M V, Templin, A, Clark, A J
A mutation partially suppressing the UV sensitivity caused by recF143 in a uvrA6 background was located at codon 37 of recA where GTG (valine) became ATG (methionine). This mutation, originally named...
Transposon-mediated conjugational transmission of nonconjugative plasmids.
Crisona, N J, Nowak, J A, Nagaishi, H, Clark, A J
When coresident with conjugative plasmid pNC21, the nonconjugative deletion F-prime pJC59, which retains the F transfer origin oriT, was transmitted to transconjugants at a frequency comparable to...
Ray, D W, Littlewood, A C, Clark, A J, Davis, J R, White, A
Some human small cell lung carcinomas (SCLC) secrete proopiomelanocortin (POMC) derived peptides, but in contrast to the pituitary, glucocorticoids fail to inhibit this hormone production. We have...
Strain JC10240 (Hfr PO45 srlC300::Tn10 recA56 thr-300 ilv-318 rpsE300) was constructed. On account of the close linkage of Tn10 to recA56, the latter can be moved to other Escherichia coli (and...
Buzzetti, R, McLoughlin, L, Lavender, P M, Clark, A J, Rees, L H
Using Northern blotting with a human genomic DNA probe for the pro-opiomelanocortin (POMC) gene, we have shown specific mRNA in normal human peripheral mononuclear cells (PBMC); the presence of...
Sandler, S J, Chackerian, B, Li, J T, Clark, A J
We have compared the recF genes from Escherichia coli K-12, Salmonella typhimurium, Pseudomonas putida, and Bacillus subtilis at the DNA and amino acid sequence levels. To do this we determined the...
Characteristics of Some Multiply Recombination-Deficient Strains of Escherichia coli
Strains of Escherichia coli have been made carrying lesions in more than one gene determining recombination. The following genotypes were constructed and verified: recC22 recB21 recA+, recC22 recB21...
Evidence that rnmB is the operator of the Escherichia coli recA gene.
Volkert, M R, Margossian, L J, Clark, A J
rnmB281 leads to high constitutive levels of recA protein such that no increase after UV-inducing treatment occurs. The mutation maps in or near the portion of recA corresponding to the NH2-terminal...
Effect of RecF protein on reactions catalyzed by RecA protein.
RecF protein is one of at least three single strand DNA (ssDNA) binding proteins which act in recombination and repair in Escherichia coli. In this paper we show that our RecF protein preparation...
Watson, C J, Gordon, K E, Robertson, M, Clark, A J
The minimal 5' regulatory region of the sheep beta-lactoglobulin gene (BLG), as defined in transgenic mice, was used to identify nuclear factors which may be involved in milk protein gene expression...
Farrell, W E, Clark, A J, Stewart, M F, Crosby, S R, White, A
We have previously reported that a human small cell lung cancer (SCLC) cell line (COR L103) that expresses the proopiomelanocortin (POMC) gene and secretes ACTH precursor peptides is relatively...
Molecular cloning of DNA from F sex factor of Escherichia coli K-12.
Skurray, R A, Nagaishi, H, Clark, A J
We describe the molecular cloning of various DNA segments generated by partial EcoRI endonuclease digestion of the sex factor F. These segments have been analyzed by agarose gel electrophoresis of...
Sequence-specific recombination of plasmid ColE1.
The conjugal transmission of plasmid ColE1 and its derivatives can lead to recA-independent recombination. The recombination is not observed during vegetative growth and takes place specifically...
The 45-kb unit of major urinary protein gene organization is a gigantic imperfect palindrome.
Bishop, J O, Selman, G G, Hickman, J, Black, L, Saunders, R D, Clark, A J
The multigene family which codes for the mouse major urinary proteins consists of about 35 genes. Most of these are members of two distinct groups, group 1 and group 2. The group 1 and group 2 genes...
Merlino, G T, Ishii, S, Whang-Peng, J, Knutsen, T, Xu, Y H, Clark, A J, ...
A431 cells have an amplification of the epidermal growth factor (EGF) receptor gene, the cellular homolog of the v-erb B oncogene, and overproduce an aberrant 2.9-kilobase RNA that encodes a portion...
Epidermal growth factor regulates the expression of its own receptor.
Clark, A J, Ishii, S, Richert, N, Merlino, G T, Pastan, I
The epidermal growth factor (EGF) receptor gene is the cellular homolog of the avian erythroblastosis virus erbB oncogene. Control of EGF receptor expression determines cellular responsiveness to EGF...
Molecular analysis of the recF gene of Escherichia coli.
Blanar, M A, Sandler, S J, Armengod, M E, Ream, L W, Clark, A J
We analyzed the nucleotide sequence of a 1.325-kilobase region of wild-type Escherichia coli containing a functional recF gene and six Tn3 mutations that inactivate recF. The analysis shows a...
Evolutionary amplification of a pseudogene.
Ghazal, P, Clark, A J, Bishop, J O
The family of mouse major urinary protein (MUP) genes has about 35 members, clustered together on chromosome 4. Most of the genes belong to two major subfamilies (group 1 and group 2) each with 12-15...
Merlino, G T, Xu, Y H, Richert, N, Clark, A J, Ishii, S, Banks-Schlegel, S, ...
The human epidermal growth factor (EGF) receptor is known to be homologous to the v-erb B oncogene protein of the avian erythroblastosis virus. Overexpression of the EGF receptor gene in A431...
Isolation of Exonuclease VIII: The Enzyme Associated with the sbcA Indirect Suppressor
Kushner, Sidney R., Nagaishi, Haruko, Clark, A. J.
recB and/or recC deficiency in Escherichia coli K-12 is indirectly suppressed by the presence of sbcA- mutations. sbcA- strains contain an increased level of an ATP-independent nuclease. Genetic and...
Two main groups of mouse major urinary protein genes, both largely located on chromosome 4.
Bishop, J O, Clark, A J, Clissold, P M, Hainey, S, Francke, U
Fourteen different major urinary protein (MUP) genomic clones from BALB/c mice were isolated. By restriction site mapping, six of these form two sets of three overlapping clones. By the criterion of...
Sequence structures of a mouse major urinary protein gene and pseudogene compared.
Clark, A J, Ghazal, P, Bingham, R W, Barrett, D, Bishop, J O
Laboratory mouse strains carry approximately 35 major urinary protein (MUP) genes per haploid genome, tightly clustered together on chromosome 4. Most belong to two main groups (Groups 1 and 2). The...
Clark, A J, Chave-Cox, A, Ma, X, Bishop, J O
Here we compare the exonic sequences of four Group 1 mouse major urinary protein (MUP) genes and four Group 1 cDNA sequences. These define seven different nucleotide sequences which differ from each...
Clark, A J, Clissold, P M, Al Shawi, R, Beattie, P, Bishop, J
The multigene family which codes for the mouse major urinary proteins (MUPs) consists of approximately 35 genes. Most of these are members of two different groups, Group 1 and Group 2, which can be...
Clark, A J, Hickman, J, Bishop, J
The multigene family which codes for the mouse major urinary proteins (MUPs) consists of approximately 35 genes. Most of these are members of two different groups, Group 1 and Group 2, which can be...
Wilde, C J, Clark, A J, Kerr, M A, Knight, C H, McClenaghan, M, Simons, J P
Mammary development and milk secretion were studied in transgenic mice which exhibited mammary tissue-specific expression of the sheep beta-lactoglobulin gene, and secreted significant quantities of...
Position-independent expression of the ovine beta-lactoglobulin gene in transgenic mice.
Whitelaw, C B, Harris, S, McClenaghan, M, Simons, J P, Clark, A J
The major milk whey protein of sheep, beta-lactoglobulin (BLG), is expressed specifically in the mammary gland in a developmentally regulated pattern. To identify the cis-acting DNA regions involved...
Secretory proteins compete for production in the mammary gland of transgenic mice.
McClenaghan, M, Springbett, A, Wallace, R M, Wilde, C J, Clark, A J
To explore the possibility that genes might compete for expression, we have studied transgenic mice producing high levels of the sheep milk protein, beta-lactoglobulin (BLG), in the mammary gland....
1. The membrane potential in intact cells of Rhodopseudomonas capsulata during photosynthesis and during dark respiration has been measured by two independent methods. 2. The light-induced and...
Mahajan, S. K., Chu, C. C., Willis, D. K., Templin, A., Clark, A. J.
We have mapped the extents of two deletion sbcA mutations which result in production of DNA exonuclease VIII (ExoVIII). One mutation, sbcA8, deletes about 140 kb of DNA which includes most of the Rac...
We showed that sufficient overexpression of the wild-type recF gene interfered with three normal cell functions: (1) UV induction of transcription from the LexA-protein-repressed sulA promoter, (2)...
Sandler, S. J., Samra, H. S., Clark, A. J.
First identified as an essential component of the φX174 in vitro DNA replication system, PriA has ATPase, helicase, translocase, and primosome-assembly activities. priA1::kan strains of Escherichia...
A Proposal for a Uniform Nomenclature in Bacterial Genetics
Demerec, M., Adelberg, E. A., Clark, A. J., Hartman, Philip E.
Genetic Analysis of a Double Male Strain of ESCHERICHIA COLI K12
Falkinham, Joseph O., Clark, A. J.
The behavior of a double male strain of Escherichia coli K12 has been compared to that of its parents and a primary F' strain carrying F14 in order to determine whether the genome of the double male,...
Diabetic impotence: studies of nocturnal erection during REM sleep.
Hosking, D J, Bennet, T, Hampton, J R, Evans, D F, Clark, A J, Robertson, G
Nocturnal erections were studied in 30 diabetic patients who complained of impotence and in 11 healthy volunteers. The maximum increase in penile circumference was measured by a penile strain-gauge...
Winn, P., Stone, T. W., Latimer, M., Hastings, M. H., Clark, A. J.
1. It has been suggested that an NMDA1 receptor subtype might be activated by N-methyl-D-aspartate (NMDA) and ibotenate and an NMDA2 subtype by NMDA or quinolinate, and that the NMDA2 site might be...
Chronic Salmonella eimsbuettel septicaemia presenting with intermittent sweats and haematuria.
Lowes, J. A., Clark, A. J., Britten, K. E.
An unusual presentation is reported of salmonellosis, frank haematuria and chronic septicaemia arising during an outbreak of food poisoning caused by Salmonella eimsbuettel. The patient whose gut was...