A. J. Ulmer

Hemoglobin Enhances the Biological Activity of Synthetic and Natural Bacterial (Endotoxic) Virulence Factors: A General Principle (2008)

J. Howe, W. Richter, L. Hawkins, M. Roessle, C. Alexander, ...

Journal article

Structural investigations into the interaction of hemoglobin and part structures with bacterial endotoxins (2008)

J. Howe, P. Garidel, M. Roessle, W. Richter, C. Alexander, ...

Journal article

Physicochemical and biological analysis of synthetic bacterial lipopeptides: Validity of the concept of ëndotoxic conformation". (2007)

A.B. Schromm, J. Howe, A.J. Ulmer, K.H. Wiesmüller, T. Seyberth, ...

Journal article

Biophysical Characterization of the Interaction of Endotoxins with Hemoglobins. (2006)

J. Howe, M. Hammer, C. Alexander, M. Roessle, K. Fournier, ...

Journal article

Abstract (1992)

Strohscheer, I., Kleinert, R., Westphal, C., Schüller, J., Werner, K., Anders, O., ...

Peer Reviewed

The Internalization Time Course of a Given Lipopolysaccharide Chemotype Does Not Correspond to Its Activation Kinetics in Monocytes

Lentschat, A., El-Samalouti, V. T., Schletter, J., Kusumoto, S., Brade, L., Rietschel, E. T., ...

The prerequisites for the initiation of pathophysiological effects of endotoxin (lipopolysaccharide [LPS]) include binding to and possibly internalization by target cells. Monocytes/macrophages are...

Binding of lipopolysaccharide (LPS) to an 80-kilodalton membrane protein of human cells is mediated by soluble CD14 and LPS-binding protein.

Schletter, J, Brade, H, Brade, L, Krüger, C, Loppnow, H, Kusumoto, S, ...

Activation of cells by bacterial lipopolysaccharide (LPS) plays a key role in the pathogenesis of gram-negative septic shock. The 55-kDa glycoprotein CD14 is known to bind LPS and initiate cell...

Glycosphingolipids from Sphingomonas paucimobilis induce monokine production in human mononuclear cells.

Krziwon, C, Zähringer, U, Kawahara, K, Weidemann, B, Kusumoto, S, Rietschel, E T, ...

Glycosphingolipids (GSL) isolated from the gram-negative lipopolysaccharide (LPS)-free bacterium Sphingomonas paucimobilis have remarkable structural similarities with LPS and its hydrophobic part,...

Specific binding of soluble peptidoglycan and muramyldipeptide to CD14 on human monocytes.

Weidemann, B, Schletter, J, Dziarski, R, Kusumoto, S, Stelter, F, Rietschel, E T, ...

Previously, we were able to show that soluble peptidoglycan (sPG)-induced monokine production in human peripheral monocytes is inhibited by anti-CD14 monoclonal antibodies and by lipid A partial...

Biological activity of synthetic phosphonooxyethyl analogs of lipid A and lipid A partial structures.

Ulmer, A J, Heine, H, Feist, W, Kusumoto, S, Kusama, T, Brade, H, ...

We investigated the biological activity of four new synthetic analogs of lipid A, termed PE-1, PE-2, PE-3, and PE-4. All compounds contain an alpha-oxyethyl-linked (-O-CH2-CH2-) phosphoryl group in...

Modulation of endotoxin-induced monokine release in human monocytes by lipid A partial structures that inhibit binding of 125I-lipopolysaccharide.

Ulmer, A J, Feist, W, Heine, H, Kirikae, T, Kirikae, F, Kusumoto, S, ...

We have previously shown that the synthetic tetraacyl precursor Ia (compound 406, LA-14-PP, or lipid IVa) was not able to induce the production of tumor necrosis factor, interleukin-1, and...

Inhibitory and stimulatory effects of Pseudomonas aeruginosa pyocyanine on human T and B lymphocytes and human monocytes.

Ulmer, A J, Pryjma, J, Tarnok, Z, Ernst, M, Flad, H D

Pyocyanine, a pigment produced by Pseudomonas aeruginosa, has dual dose-dependent stimulatory as well as inhibitory effects on immune responses in vitro as measured by DNA synthesis of human T and B...

Inhibition of endotoxin-induced interleukin-6 production by synthetic lipid A partial structures in human peripheral blood mononuclear cells.

Wang, M H, Flad, H D, Feist, W, Brade, H, Kusumoto, S, Rietschel, E T, ...

The effect of two synthetic lipid A partial structures, compound 406 (or LA-14-PP, identical in structure to the lipid A precursor, known as Ia or IVa) and compound 401 (lipid X), on the in vitro...

Soluble peptidoglycan-induced monokine production can be blocked by anti-CD14 monoclonal antibodies and by lipid A partial structures.

Weidemann, B, Brade, H, Rietschel, E T, Dziarski, R, Bazil, V, Kusumoto, S, ...

We have investigated the interaction of soluble peptidoglycan (sPG), in comparison with lipopolysaccharide (LPS), with human mononuclear cells (MNC) by determining the capacity of sPG to induce...

The Internalization Time Course of a Given Lipopolysaccharide Chemotype Does Not Correspond to Its Activation Kinetics in Monocytes

Lentschat, A., El-Samalouti, V. T., Schletter, J., Kusumoto, S., Brade, L., Rietschel, E. T., ...

The prerequisites for the initiation of pathophysiological effects of endotoxin (lipopolysaccharide [LPS]) include binding to and possibly internalization by target cells. Monocytes/macrophages are...

Binding of lipopolysaccharide (LPS) to an 80-kilodalton membrane protein of human cells is mediated by soluble CD14 and LPS-binding protein.

Schletter, J, Brade, H, Brade, L, Krüger, C, Loppnow, H, Kusumoto, S, ...

Activation of cells by bacterial lipopolysaccharide (LPS) plays a key role in the pathogenesis of gram-negative septic shock. The 55-kDa glycoprotein CD14 is known to bind LPS and initiate cell...

Glycosphingolipids from Sphingomonas paucimobilis induce monokine production in human mononuclear cells.

Krziwon, C, Zähringer, U, Kawahara, K, Weidemann, B, Kusumoto, S, Rietschel, E T, ...

Glycosphingolipids (GSL) isolated from the gram-negative lipopolysaccharide (LPS)-free bacterium Sphingomonas paucimobilis have remarkable structural similarities with LPS and its hydrophobic part,...

Specific binding of soluble peptidoglycan and muramyldipeptide to CD14 on human monocytes.

Weidemann, B, Schletter, J, Dziarski, R, Kusumoto, S, Stelter, F, Rietschel, E T, ...

Previously, we were able to show that soluble peptidoglycan (sPG)-induced monokine production in human peripheral monocytes is inhibited by anti-CD14 monoclonal antibodies and by lipid A partial...

Biological activity of synthetic phosphonooxyethyl analogs of lipid A and lipid A partial structures.

Ulmer, A J, Heine, H, Feist, W, Kusumoto, S, Kusama, T, Brade, H, ...

We investigated the biological activity of four new synthetic analogs of lipid A, termed PE-1, PE-2, PE-3, and PE-4. All compounds contain an alpha-oxyethyl-linked (-O-CH2-CH2-) phosphoryl group in...

Modulation of endotoxin-induced monokine release in human monocytes by lipid A partial structures that inhibit binding of 125I-lipopolysaccharide.

Ulmer, A J, Feist, W, Heine, H, Kirikae, T, Kirikae, F, Kusumoto, S, ...

We have previously shown that the synthetic tetraacyl precursor Ia (compound 406, LA-14-PP, or lipid IVa) was not able to induce the production of tumor necrosis factor, interleukin-1, and...

Inhibitory and stimulatory effects of Pseudomonas aeruginosa pyocyanine on human T and B lymphocytes and human monocytes.

Ulmer, A J, Pryjma, J, Tarnok, Z, Ernst, M, Flad, H D

Pyocyanine, a pigment produced by Pseudomonas aeruginosa, has dual dose-dependent stimulatory as well as inhibitory effects on immune responses in vitro as measured by DNA synthesis of human T and B...

Inhibition of endotoxin-induced interleukin-6 production by synthetic lipid A partial structures in human peripheral blood mononuclear cells.

Wang, M H, Flad, H D, Feist, W, Brade, H, Kusumoto, S, Rietschel, E T, ...

The effect of two synthetic lipid A partial structures, compound 406 (or LA-14-PP, identical in structure to the lipid A precursor, known as Ia or IVa) and compound 401 (lipid X), on the in vitro...

Soluble peptidoglycan-induced monokine production can be blocked by anti-CD14 monoclonal antibodies and by lipid A partial structures.

Weidemann, B, Brade, H, Rietschel, E T, Dziarski, R, Bazil, V, Kusumoto, S, ...

We have investigated the interaction of soluble peptidoglycan (sPG), in comparison with lipopolysaccharide (LPS), with human mononuclear cells (MNC) by determining the capacity of sPG to induce...

Antibody-induced modulation of CD26 surface expression.

Mattern, T, Reich, C, Duchrow, M, Ansorge, S, Ulmer, A J, Flad, H D

The ability of different anti-CD26 monoclonal antibodies to modulate the expression of CD26 on human T lymphocytes was investigated. By means of a new non-radioactive method using fluorescein...

Pentoxifylline: a potent inhibitor of IL-2 and IFN-gamma biosynthesis and BCG-induced cytotoxicity.

Thanhäuser, A, Reiling, N, Böhle, A, Toellner, K M, Duchrow, M, Scheel, D, ...

In the present study we investigated the influence of pentoxifylline (POF) on bacillus Calmette-Guérin (BCG)- and phytohaemagglutinin (PHA)-induced DNA synthesis and cytokine release, and...

Immunoglobulin production of human lymphocytes stimulated by Staphylococcus aureus Cowan I and pokeweed mitogen: differential effects of recombinant interleukin-2.

Pryjma, J, Flad, H D, Ernst, M, Brandt, E, Ulmer, A J

The number of immunoglobulin-secreting cells (ISC) upon stimulation with pokeweed mitogen (PWM) or Staphylococcus aureus Cowan I (SAC) in recombinant interleukin-2 (rIL-2)-supplemented cultures of...

One-stage stimulation of human T-lymphocyte colony-forming units (TL-CFU) in a micro agar culture in glass capillaries.

Ulmer, A J, Flad, H D

A one-stage micro-culture assay in glass capillary tubes was developed for colony growth of human T lymphocytes. Colony growth by this method did not depend on the addition of growth factors, human...

Stimulation of colony formation and growth factor production of human T lymphocytes by wheat germ lectin.

Ulmer, A J, Scholz, W, Flad, H D

Wheat germ lectin (WGL) induced proliferation of T-lymphocyte colony-forming units (TL-CFU) from human peripheral blood mononuclear cells (PBMC) in a one stage agar culture needing higher cell...

Suppression of Staphylococcus aureus Cowan I-induced immunoglobulin synthesis in vitro: discrimination between the presence of suppressor T cell precursors and effectors.

Pryjma, J, Pituch-Noworolska, A, Flad, H D, Ulmer, A J, Ernst, M

In co-cultures with control cells lymphocytes obtained from some patients with hypogammaglobulinaemia can suppress PWM but not S. aureus Cowan I-induced polyclonal immunoglobulin production. When...