Carothers, A M, Zhen, W, Mucha, J, Zhang, Y J, Santella, R M, Grunberger, D, ...
We evaluated the formation and removal of (+-)-3 alpha,4 beta-dihydroxy-1 alpha,2 alpha-epoxy-1,2,3,4- tetrahydrobenzo[c]phenanthrene (BcPHDE)-DNA adducts in two Chinese hamster ovary (CHO) cell...
Carothers, A M, Mucha, J, Grunberger, D
We previously showed that the preferred mutation induced by (+/-)-3 alpha,4 beta-dihydroxy-1 alpha,2 alpha-epoxy- 1,2,3,4-tetrahydrobenzo[c]phenanthrene (BcPHDE) in the dihydrofolate reductase gene...
Carothers, A M, Urlaub, G, Mucha, J, Harvey, R G, Chasin, L A, Grunberger, D
Cultured Chinese hamster ovary (CHO) cells were treated with the polycyclic aromatic hydrocarbon racemic 3 alpha,4 beta-dihydroxy-1 alpha,2 alpha-epoxy-1,2,3,4- tetrahydrobenzo[c]phenanthrene....
Palchaudhuri, S, McFall, E, Carothers, A M
We have obtained two classes of double lysogens that on induction yield higher titers of lambda-d dsd transducing phage than of helper phage. One class was obtained by lysogenization of strain EM6116...
Positive control in the D-serine deaminase system of Escherichia coli K-12.
Bloom, F R, McFall, E, Young, M C, Carothers, A M
Two new types of D-serine deaminase (Dsdase)-negative mutants have been isolated and characterized. The first fails to synthesize a functional dsdC gene product as a result of dsdC- (regulator...
Carothers, A M, McFall, E, Palchaudhuri, S
The genes dsdA, dsdO, and dsdC have been located on a 3.0-kilobase pair (kb) fragment of the Escherichia coli chromosome by a combination of techniques. The loci were first cloned onto lambda and...
Position effect on expression of dsd genes cloned onto multicopy plasmids.
Carothers, A M, Heincz, M C, McFall, E
In the D-serine deaminase system of Escherichia coli, which is regulated by positive control, we have fouand a complete lack of trans activation in vivo with multicopy dsd hybrid plasmids. A PLASmid...
Banerjee, R, Carothers, A M, Grunberger, D
It has been demonstrated that certain alternating purine and pyrimidine sequences may assume a left-handed Z-DNA conformation. In order to evaluate the possibility that Z-DNA is involved in the...
Structure of the dihydrofolate reductase gene in Chinese hamster ovary cells.
Carothers, A M, Urlaub, G, Ellis, N, Chasin, L A
Overlapping recombinant lambda 1059 phages carrying regions of the dhfr locus from the amplified Chinese hamster ovary (CHO) cell clone MK42 have been isolated. In addition, dhfr cDNAs from this cell...
Carothers, A M, Urlaub, G, Grunberger, D, Chasin, L A
Point mutants induced with a variety of mutagens at the dihydrofolate reductase (dhfr) locus in Chinese hamster ovary (CHO) cells were screened for aberrantly spliced dhfr mRNA by RNase protection...
Mitchell, P J, Carothers, A M, Han, J H, Harding, J D, Kas, E, Venolia, L, ...
Transcription of the 26-kilobase (kb) dihydrofolate reductase (dhfr) gene in CHO cells is initiated at two sites: a major site (approximately 85% of the dhfr mRNA) at -63 relative to the translation...
Carothers, A M, Urlaub, G, Steigerwalt, R W, Chasin, L A, Grunberger, D
To determine the types of alterations in gene structure that are induced by the carcinogen 2-(N-acetoxy-N-acetyl)aminofluorene, we used this compound to generate mutations at the dihydrofolate...
Urlaub, G, Carothers, A M, Chasin, L A
A method for the efficient cloning of single-copy genes from restriction digests of mammalian DNA is described. The method is illustrated by the cloning of several mutant genes as well as the...
Carothers, A M, Zhen, W, Mucha, J, Zhang, Y J, Santella, R M, Grunberger, D, ...
We evaluated the formation and removal of (+-)-3 alpha,4 beta-dihydroxy-1 alpha,2 alpha-epoxy-1,2,3,4- tetrahydrobenzo[c]phenanthrene (BcPHDE)-DNA adducts in two Chinese hamster ovary (CHO) cell...
Carothers, A M, Mucha, J, Grunberger, D
We previously showed that the preferred mutation induced by (+/-)-3 alpha,4 beta-dihydroxy-1 alpha,2 alpha-epoxy- 1,2,3,4-tetrahydrobenzo[c]phenanthrene (BcPHDE) in the dihydrofolate reductase gene...
Carothers, A M, Urlaub, G, Mucha, J, Harvey, R G, Chasin, L A, Grunberger, D
Cultured Chinese hamster ovary (CHO) cells were treated with the polycyclic aromatic hydrocarbon racemic 3 alpha,4 beta-dihydroxy-1 alpha,2 alpha-epoxy-1,2,3,4- tetrahydrobenzo[c]phenanthrene....
Palchaudhuri, S, McFall, E, Carothers, A M
We have obtained two classes of double lysogens that on induction yield higher titers of lambda-d dsd transducing phage than of helper phage. One class was obtained by lysogenization of strain EM6116...
Positive control in the D-serine deaminase system of Escherichia coli K-12.
Bloom, F R, McFall, E, Young, M C, Carothers, A M
Two new types of D-serine deaminase (Dsdase)-negative mutants have been isolated and characterized. The first fails to synthesize a functional dsdC gene product as a result of dsdC- (regulator...
Carothers, A M, McFall, E, Palchaudhuri, S
The genes dsdA, dsdO, and dsdC have been located on a 3.0-kilobase pair (kb) fragment of the Escherichia coli chromosome by a combination of techniques. The loci were first cloned onto lambda and...
Position effect on expression of dsd genes cloned onto multicopy plasmids.
Carothers, A M, Heincz, M C, McFall, E
In the D-serine deaminase system of Escherichia coli, which is regulated by positive control, we have fouand a complete lack of trans activation in vivo with multicopy dsd hybrid plasmids. A PLASmid...
Banerjee, R, Carothers, A M, Grunberger, D
It has been demonstrated that certain alternating purine and pyrimidine sequences may assume a left-handed Z-DNA conformation. In order to evaluate the possibility that Z-DNA is involved in the...
Structure of the dihydrofolate reductase gene in Chinese hamster ovary cells.
Carothers, A M, Urlaub, G, Ellis, N, Chasin, L A
Overlapping recombinant lambda 1059 phages carrying regions of the dhfr locus from the amplified Chinese hamster ovary (CHO) cell clone MK42 have been isolated. In addition, dhfr cDNAs from this cell...
Carothers, A M, Urlaub, G, Grunberger, D, Chasin, L A
Point mutants induced with a variety of mutagens at the dihydrofolate reductase (dhfr) locus in Chinese hamster ovary (CHO) cells were screened for aberrantly spliced dhfr mRNA by RNase protection...
Mitchell, P J, Carothers, A M, Han, J H, Harding, J D, Kas, E, Venolia, L, ...
Transcription of the 26-kilobase (kb) dihydrofolate reductase (dhfr) gene in CHO cells is initiated at two sites: a major site (approximately 85% of the dhfr mRNA) at -63 relative to the translation...
Carothers, A M, Urlaub, G, Steigerwalt, R W, Chasin, L A, Grunberger, D
To determine the types of alterations in gene structure that are induced by the carcinogen 2-(N-acetoxy-N-acetyl)aminofluorene, we used this compound to generate mutations at the dihydrofolate...
Urlaub, G, Carothers, A M, Chasin, L A
A method for the efficient cloning of single-copy genes from restriction digests of mammalian DNA is described. The method is illustrated by the cloning of several mutant genes as well as the...