Metabolism and growth yields in Bacteroides ruminicola strain b14.
Howlett, M R, Mountfort, D O, Turner, K W, Roberton, A M
Metabolism of D-glucose by Bacteroides ruminicola subsp. brevis, strain B14, has been examined. Growth yield studies gave molar growth yields, corrected for storage polysaccharide, of approximately...
Fructose 6-phosphate phosphorylation in Bacteroides species.
6-Phosphofructokinase (6-PFK) activities have been measured in cell extracts from a number of Bacteroides species. Two main types of 6-PFK were found: an ATP-linked 6-PFK and a PPi-linked 6-PFK. In...
Degradation of pig gastric and colonic mucins by bacteria isolated from the pig colon.
Stanley, R A, Ram, S P, Wilkinson, R K, Roberton, A M
Mucin degradation was studied with one Clostridium (RS42) and two Bacteroides (RS2 and RS13) strains isolated from the pig colon mucosa. Mucins from pig colon and stomach were prepared in their...
In vitro utilization of mucin by Bacteroides fragilis.
A method for isolating pig colon mucin in a soluble high-molecular-weight form, suitable for addition to bacterial growth media, is described. This preparation was utilized as a sole carbohydrate...
Drumm, B, Roberton, A M, Sherman, P M
Intestinal mucus is postulated to play a role in preventing colonization of the gastrointestinal tract by microbial pathogens. To evaluate the ability of both crude mucus and purified mucin, a...
Adenosine Triphosphate Pools in Methanobacterium
Certain aspects of adenosine triphosphate (ATP) metabolism in the strict anaerobe Methanobacterium strain M.o.H. have been investigated. Results of growth yield studies suggest that ATP conservation...
A glycosulphatase that removes sulphate from mucus glycoprotein.
Roberton, A M, McKenzie, C G, Sharfe, N, Stubbs, L B
A novel glycosulphatase has been purified from a mucus glycopeptide-degrading Prevotella from the colon. The purified enzyme removed inorganic [35S]sulphate from 35S-labelled native rat gastric mucus...
Roberton, A M, Mantle, M, Fahim, R E, Specian, R D, Bennick, A, Kawagishi, S, ...
The existence of a discrete 'link' peptide in epithelial mucins has been debated for many years. There is evidence that at least some mucins contain a specific 'link' peptide (or glycopeptide) that...
Blackmore, R, Roberton, A M, Brittain, T
The bacterium Wolinella succinogenes produces a nitrite reductase enzyme that can be purified to homogeneity in high yield by a combination of detergent extraction, hydroxyapatite chromatography and...
The chemical nature of the site of action of dicyclohexylcarbodi-imide in mitochondria
Knight, I. G., Holloway, Caroline T., Roberton, A. M., Beechey, R. B.
Roberton, A. M., Holloway, Caroline T., Knight, I. G., Beechey, R. B.
1. The effects of dicyclohexylcarbodi-imide, oligomycin A and aurovertin on enzyme systems related to respiratory-chain phosphorylation were compared. Dicyclohexylcarbodi-imide and oligomycin A have...
Metabolism and growth yields in Bacteroides ruminicola strain b14.
Howlett, M R, Mountfort, D O, Turner, K W, Roberton, A M
Metabolism of D-glucose by Bacteroides ruminicola subsp. brevis, strain B14, has been examined. Growth yield studies gave molar growth yields, corrected for storage polysaccharide, of approximately...
Fructose 6-phosphate phosphorylation in Bacteroides species.
6-Phosphofructokinase (6-PFK) activities have been measured in cell extracts from a number of Bacteroides species. Two main types of 6-PFK were found: an ATP-linked 6-PFK and a PPi-linked 6-PFK. In...
Degradation of pig gastric and colonic mucins by bacteria isolated from the pig colon.
Stanley, R A, Ram, S P, Wilkinson, R K, Roberton, A M
Mucin degradation was studied with one Clostridium (RS42) and two Bacteroides (RS2 and RS13) strains isolated from the pig colon mucosa. Mucins from pig colon and stomach were prepared in their...
In vitro utilization of mucin by Bacteroides fragilis.
A method for isolating pig colon mucin in a soluble high-molecular-weight form, suitable for addition to bacterial growth media, is described. This preparation was utilized as a sole carbohydrate...
Drumm, B, Roberton, A M, Sherman, P M
Intestinal mucus is postulated to play a role in preventing colonization of the gastrointestinal tract by microbial pathogens. To evaluate the ability of both crude mucus and purified mucin, a...
Adenosine Triphosphate Pools in Methanobacterium
Certain aspects of adenosine triphosphate (ATP) metabolism in the strict anaerobe Methanobacterium strain M.o.H. have been investigated. Results of growth yield studies suggest that ATP conservation...
A glycosulphatase that removes sulphate from mucus glycoprotein.
Roberton, A M, McKenzie, C G, Sharfe, N, Stubbs, L B
A novel glycosulphatase has been purified from a mucus glycopeptide-degrading Prevotella from the colon. The purified enzyme removed inorganic [35S]sulphate from 35S-labelled native rat gastric mucus...
Roberton, A M, Mantle, M, Fahim, R E, Specian, R D, Bennick, A, Kawagishi, S, ...
The existence of a discrete 'link' peptide in epithelial mucins has been debated for many years. There is evidence that at least some mucins contain a specific 'link' peptide (or glycopeptide) that...
Blackmore, R, Roberton, A M, Brittain, T
The bacterium Wolinella succinogenes produces a nitrite reductase enzyme that can be purified to homogeneity in high yield by a combination of detergent extraction, hydroxyapatite chromatography and...
The chemical nature of the site of action of dicyclohexylcarbodi-imide in mitochondria
Knight, I. G., Holloway, Caroline T., Roberton, A. M., Beechey, R. B.
Roberton, A. M., Holloway, Caroline T., Knight, I. G., Beechey, R. B.
1. The effects of dicyclohexylcarbodi-imide, oligomycin A and aurovertin on enzyme systems related to respiratory-chain phosphorylation were compared. Dicyclohexylcarbodi-imide and oligomycin A have...