Evans, A, Clements, K, Maxwell, A, Bishop, H, Hanby, A, Lawrence, G, ...
No abstract available.
The phytotoxin albicidin is a novel inhibitor of DNA gyrase (2007)
Hashimi, SM, Wall, MK, Smith, AB, Maxwell, A, Birch, RG
Xanthomonas albilineans produces a family of polyketide-peptide compounds called albicidins which are highly potent antibiotics and phytotoxins as a result of their inhibition of prokaryotic DNA...
The phytotoxin albicidin is a novel inhibitor of DNA gyrase (2007)
Hashimi, S. M., Wall, M. K., Smith, A. B., Maxwell, A., Birch, R. G.
Evans, A, Clements, K, Maxwell, A, Dobson, H, Wallis, M, Lawrence, G, ...
No abstract available.
THE DEVELOPMENT AND OPERATION OF A DYNAMIC SPECTRUM ANALYZER (2005)
A survey is presented of the research work directed towards the study of radio burst emissions from the sun with a dynamic spectrum analyzer consisting of a series of sweep-fre-q ency receivers....
Teaching Computer Vision to Computer Scientists: (2003)
Computer vision is a broad-based field of computer science that requires students to understand and integrate knowledge from numerous disciplines. Computer science [CS] majors, however, do not...
E27/CS27: Computer Vision, Fall 1999 (1999)
Introduction . What is computer vision (tree digram) . Difference with image processing: for human v. computer consumption . history of computer vision . 60's first work with images . 70's...
RADIO EVIDENCE FOR SOLAR CORPUSCULAR EMISSION, (1998)
Maxwell, A., Defouw, R. J., Cummings, P.
This paper reviews the relation of solar radio bursts of spectral type IV to the ejection of relativistic and sub-relativistic solar particles; the relation of bursts of types II and IV to the...
Crystal structure of the breakage-reunion domain of DNA gyrase. (1997)
Morais-Cabral, J, Jackson, A, Smith, C, Shikotra, N, Maxwell, A, Liddington, R
Interaction of CO with Pd Clusters Supported on a Thin Alumina Film (1996)
Sandell,A., Libuda,J., Brühwiler,P., Andersson,S., Bäumer,M., Maxwell,A., ...
Electron Spectroscopy Studies of Small Deposited Metal Particles (1995)
Sandell,A., Libuda,J., Brühwiler,P., Andersson,S., Bäumer,M., Maxwell,A., ...
A 1152 channel timing system for an electromagnetic calorimeter readout (1990)
Bonesini, M, Bonvin, E, Booth, P S L, Carroll, L J, Cass, A J, Cavalli, D, ...
Prompt photon production by pi- and pi+ on protons at 280 GeV (1990)
Bonesini, M, Bonvin, E, Bortoletto, Daniela, Booth, P S L, Carroll, L J, Cass, A J, ...
High transverse momentum prompt photon production by pi- and pi+ on protons at 280 GeV/c (1990)
Bonesini, M, Bonvin, E, Booth, P S L, Bortoletto, Daniela, Carroll, L J, Cass, A J, ...
Study of the performance of a large sample of Philips photomultipliers type XP2972 (1990)
Bonesini, M, Bonvin, E, Booth, P S L, Carroll, L J, Cavalli, D, Cecchet, G, ...
High p$_{T}$ trigger electronics for a large orthogonal readout electromagnetic calorimeter (1990)
Béné, P, Bonesini, M, Bonvin, E, Bovier, J, Carroll, L J, Cass, A J, ...
High transverse momentum pi0 production by pi- and pi+ on protons at 280 GeV/c (1990)
Bonesini, M, Bonvin, E, Booth, P S L, Bortoletto, Daniela, Carroll, L J, Cass, A J, ...
Calibration of an electromagnetic calorimeter for direct photon physics (1990)
Bonesini, M, Bonvin, E, Carroll, L J, Cass, A J, Cavalli, D, Costa, G, ...
Bonesini, M, Bonvin, E, Booth, P S L, Bopp, R, Carroll, L J, Cass, A J, ...
Identifying the catalytic residue of the ATPase reaction of DNA gyrase.
We propose a mechanism for the hydrolysis of ATP by the DNA gyrase B protein in which Glu42 acts as a general base and His38 has a role in aligning and polarizing the glutamate residue. We have...
Probing the role of the ATP-operated clamp in the strand-passage reaction of DNA gyrase.
The high-resolution structure of the 43 kDa N-terminal fragment of the DNA gyrase B protein shows a large cavity within the protein dimer. The approximate size of this cavity is 20 A, suggesting it...
DNA gyrase can cleave short DNA fragments in the presence of quinolone drugs.
Cove, M E, Tingey, A P, Maxwell, A
We have analysed the DNA cleavage reaction of DNA gyrase using oligonucleotides annealed to a single-stranded M13 derivative containing a preferred gyrase cleavage site. We find that gyrase can...
Mode of action of GR122222X, a novel inhibitor of bacterial DNA gyrase.
Oram, M, Dosanjh, B, Gormley, N A, Smith, C V, Fisher, L M, Maxwell, A, ...
GR122222X is a potent inhibitor of the supercoiling reaction of bacterial DNA gyrase. We show that this compound binds stoichiometrically to inactivate the ATPase activity of a 43-kDa N-terminal...
Binding of the quinolone drug norfloxacin to gyrase and DNA has been investigated. We have detected binding to gyrase-DNA complex but find no significant binding to either gyrase or DNA alone. Enzyme...
Using the techniques of gap misrepair mutagenesis and site-directed mutagenesis, we have generated two novel quinolone resistance mutations of the Escherichia coli DNA gyrase A protein. DNA...
Maxwell, A, Craigie, R, Mizuuchi, K
A DNA strand-transfer reaction is an early step in the transposition of phage Mu. It has been shown that an efficient reaction in vitro requires, in addition to buffer and salt, only the Mu A...
We have used the technique of hydroxyl radical footprinting to probe the complex between DNA gyrase and a 198 bp DNA fragment containing the preferred gyrase cleavage site from plasmid pBR322. We...
The C-terminal domain of the Escherichia coli DNA gyrase A subunit is a DNA-binding protein.
We have constructed a clone which over-produces a 33 kDa protein representing the C-terminal portion of the Escherichia coli DNA gyrase A subunit. This protein has no enzymic activity of its own, but...
The cleavage of DNA at phosphorothioate internucleotidic linkages by DNA gyrase.
Dobbs, S T, Cullis, P M, Maxwell, A
We have constructed a plasmid which contains 22 copies of a 147 bp DNA fragment which contains the major DNA gyrase cleavage site from plasmid pBR322 (located at base-pair 990). We have found that...
DNA gyrase can supercoil DNA circles as small as 174 base pairs.
DNA gyrase introduces negative supercoils into closed-circular DNA using the free energy of ATP hydrolysis. Consideration of steric and thermodynamic aspects of the supercoiling reaction indicates...
Lewis, R J, Singh, O M, Smith, C V, Skarzynski, T, Maxwell, A, Wonacott, A J, ...
This study describes the first crystal structures of a complex between a DNA topoisomerase and a drug. We present the structures of a 24 kDa N-terminal fragment of the Escherichia coli DNA gyrase B...
Pernicious anaemia autoantibody to gastric parietal cells: Immunofluorescence test with rat stomach
Rat stomach provided an excellent substrate for immunofluorescence testing of gastric parietal cell autoantibodies in 65 human sera. The results of similar tests using human stomach corresponded...
The SalGI restriction endonuclease. Mechanism of DNA cleavage.
The cleavage of supercoiled DNA of plasmid pMB9 by restriction endonuclease SalGI has been studied. Under the optimal conditions for this reaction, the only product is the linear form of the DNA, in...
The SalGI restriction endonuclease. Enzyme specificity.
We have analysed the kinetics of DNA cleavage in the reaction between the SalGI restriction endonuclease and plasmid pMB9. This reaction is subject to competitive inhibition by DNA sequences outside...
Identifying the catalytic residue of the ATPase reaction of DNA gyrase.
We propose a mechanism for the hydrolysis of ATP by the DNA gyrase B protein in which Glu42 acts as a general base and His38 has a role in aligning and polarizing the glutamate residue. We have...
Probing the role of the ATP-operated clamp in the strand-passage reaction of DNA gyrase.
The high-resolution structure of the 43 kDa N-terminal fragment of the DNA gyrase B protein shows a large cavity within the protein dimer. The approximate size of this cavity is 20 A, suggesting it...
DNA gyrase can cleave short DNA fragments in the presence of quinolone drugs.
Cove, M E, Tingey, A P, Maxwell, A
We have analysed the DNA cleavage reaction of DNA gyrase using oligonucleotides annealed to a single-stranded M13 derivative containing a preferred gyrase cleavage site. We find that gyrase can...
Mode of action of GR122222X, a novel inhibitor of bacterial DNA gyrase.
Oram, M, Dosanjh, B, Gormley, N A, Smith, C V, Fisher, L M, Maxwell, A, ...
GR122222X is a potent inhibitor of the supercoiling reaction of bacterial DNA gyrase. We show that this compound binds stoichiometrically to inactivate the ATPase activity of a 43-kDa N-terminal...
Binding of the quinolone drug norfloxacin to gyrase and DNA has been investigated. We have detected binding to gyrase-DNA complex but find no significant binding to either gyrase or DNA alone. Enzyme...
Using the techniques of gap misrepair mutagenesis and site-directed mutagenesis, we have generated two novel quinolone resistance mutations of the Escherichia coli DNA gyrase A protein. DNA...
Maxwell, A, Craigie, R, Mizuuchi, K
A DNA strand-transfer reaction is an early step in the transposition of phage Mu. It has been shown that an efficient reaction in vitro requires, in addition to buffer and salt, only the Mu A...
We have used the technique of hydroxyl radical footprinting to probe the complex between DNA gyrase and a 198 bp DNA fragment containing the preferred gyrase cleavage site from plasmid pBR322. We...
The C-terminal domain of the Escherichia coli DNA gyrase A subunit is a DNA-binding protein.
We have constructed a clone which over-produces a 33 kDa protein representing the C-terminal portion of the Escherichia coli DNA gyrase A subunit. This protein has no enzymic activity of its own, but...
The cleavage of DNA at phosphorothioate internucleotidic linkages by DNA gyrase.
Dobbs, S T, Cullis, P M, Maxwell, A
We have constructed a plasmid which contains 22 copies of a 147 bp DNA fragment which contains the major DNA gyrase cleavage site from plasmid pBR322 (located at base-pair 990). We have found that...
DNA gyrase can supercoil DNA circles as small as 174 base pairs.
DNA gyrase introduces negative supercoils into closed-circular DNA using the free energy of ATP hydrolysis. Consideration of steric and thermodynamic aspects of the supercoiling reaction indicates...
Lewis, R J, Singh, O M, Smith, C V, Skarzynski, T, Maxwell, A, Wonacott, A J, ...
This study describes the first crystal structures of a complex between a DNA topoisomerase and a drug. We present the structures of a 24 kDa N-terminal fragment of the Escherichia coli DNA gyrase B...
Pernicious anaemia autoantibody to gastric parietal cells: Immunofluorescence test with rat stomach
Rat stomach provided an excellent substrate for immunofluorescence testing of gastric parietal cell autoantibodies in 65 human sera. The results of similar tests using human stomach corresponded...
The SalGI restriction endonuclease. Mechanism of DNA cleavage.
The cleavage of supercoiled DNA of plasmid pMB9 by restriction endonuclease SalGI has been studied. Under the optimal conditions for this reaction, the only product is the linear form of the DNA, in...
The SalGI restriction endonuclease. Enzyme specificity.
We have analysed the kinetics of DNA cleavage in the reaction between the SalGI restriction endonuclease and plasmid pMB9. This reaction is subject to competitive inhibition by DNA sequences outside...
Temperature-sensitive suppressor mutations of the Escherichia coli DNA gyrase B protein.
Blance, S. J., Williams, N. L., Preston, Z. A., Bishara, J., Smyth, M. S., Maxwell, A.
Escherichia coli strain LE316 contains a mutation in gyrB that results in the substitution of Val164 to Gly and confers both chlorobiocin resistance and temperature sensitivity. Selection for...
Retransplantation in a patient with cystic fibrosis
McCloskey, M, Maxwell, A, Hall, V, Redmond, A, Elborn, J
A patient with cystic fibrosis is described who requested a third lung transplant. The medical and ethical issues involved are discussed.
Improved prognosis for congenital nephrotic syndrome of the Finnish type in Irish families
Savage, J, Jefferson, J, Maxwell, A, Hughes, A, Shanks, J, Gill, D
Congenital nephrotic syndrome of the Finnish type is a rare autosomal recessive disease with a high infant mortality without aggressive treatment. The biochemical basis of the disease is not...
Late referral for assessment of renal failure
Kee, F., Reaney, E., Maxwell, A, Fogarty, D., Savage, G., Patterson, C, ...
It has been recommended that adult patients with a serum creatinine above 150 µmol/l should be referred to a nephrologist for specialist assessment. This study ascertained all patients in Northern...