Role of antibody and T cells in the long-term inhibition of IgE synthesis.
We have shown that the long-term inhibition of IgE synthesis associated with perinatal inoculation of syngeneic IgE is accompanied by the synthesis of autoantibodies to IgE. Synthesis of IgE can also...
IgE-secreting cells in the thymus: correlation with induction of tolerance to IgE.
We have shown previously that normal mice become tolerant to endogenous IgE when they are approximately 2 weeks old and that this corresponds closely with the initial appearance of IgE in serum....
Lascombe, M B, Alzari, P M, Poljak, R J, Nisonoff, A
The three-dimensional structure of FabR19.9 from a well-characterized anti-p-azobenzenearsonate monoclonal antibody has been determined by x-ray diffraction techniques in two crystalline forms (I and...
Inoculation of syngeneic IgE into 2- to 12-day-old mice results in prolonged synthesis of anti-IgE antibodies without further challenge. These anti-IgE antibodies may be largely responsible for the...
Induction and properties of syngeneic murine anti-immunoglobulin D.
Haba, S, Thorbecke, G J, Nisonoff, A
High titers of autoantibodies directed to isotypic determinants of IgD were produced by inoculation of syngeneic monoclonal IgD, conjugated covalently to keyhole limpet hemocyanin, into adult or...
Wang, A. C., Wilson, S. K., Hopper, J. E., Fudenberg, H. H., Nisonoff, A.
Previous work indicated that the light chains of a monotypic immunoglobulins G2-K and M-K from a single patient (Ti1) are identical. Our present data show that the monotypic immunoglobulins G and M...
Lascombe, M B, Alzari, P M, Boulot, G, Saludjian, P, Tougard, P, Berek, C, ...
The crystal structure of Fab R19.9, derived from an anti-p-azobenzenearsonate monoclonal antibody, has been determined and refined to 2.8-A resolution by x-ray crystallographic techniques. Monoclonal...
Isotype switching by a microinjected mu immunoglobulin heavy chain gene in transgenic mice.
Durdik, J, Gerstein, R M, Rath, S, Robbins, P F, Nisonoff, A, Selsing, E
Immunization of transgenic mice carrying an immunoglobulin mu heavy chain resulted in a response dominated by expression of the transgene variable region. Unexpectedly, in a large proportion of the...
Identity of the V kappa 10-Ars-A gene segments of the A/J and BALB/c strains.
Meek, K, Sanz, I, Rathbun, G, Nisonoff, A, Capra, J D
To characterize the light chain gene segments involved in the murine immune response to keyhole limpet hemocyanin p-azophenylarsonate (Ars), we have determined the amino acid and/or nucleotide...
Induction of high titers of anti-IgE by immunization of inbred mice with syngeneic IgE.
We have generated high titers (up to 1 mg/ml) of antibodies to isotypic determinants of IgE by immunization of A/J mice with syngeneic monoclonal IgE conjugated to keyhole limpet hemocyanin. As much...
Sy, M S, Brown, A, Bach, B A, Benacerraf, B, Gottlieb, P D, Nisonoff, A, ...
Data are reported on the genetic control and structure of antigen-specific suppressive molecules obtained from azobenzenearsonate (ABA)-specific suppressor T cells (Ts). Ts-derived suppressor factors...
Influence of helper T cells on the expression of a murine intrastrain crossreactive idiotype.
Hathcock, K S, Gurish, M F, Nisonoff, A, Conger, J D, Hodes, R J
The requirement for idiotype-specific helper T (Th) cells in the generation of a major intrastrain crossreactive idiotype was investigated. This idiotype, designated CRIA, is associated with a large...
Robbins, P F, Rosen, E M, Haba, S, Nisonoff, A
We describe here an intrastrain, cross-reactive idiotype (CRI), CRID, associated with anti-p-azobenzenearsonate antibodies of the A/J strain of mouse and distinguishable, by some but not by all of...
Romano, T J, Lerman, S P, Bangasser, S, Thorbecke, G J, Nisonoff, A
Adoptive anti-trinitrophenyl (Tnp) responses were elicited from Tnp-hemocyanin (Tnp-KLH)-primed cells by challenge with an immune complex of KLH and a Tnp conjugate of the Fab fragment of rabbit...
Levin, A. S., Fudenberg, H. H., Hopper, J. E., Wilson, S. K., Nisonoff, A.
Two distinct paraproteins (IgG2-K and IgM-K) from one patient shared identical light chains and significant portions of the variable regions of the heavy chains. Idiotypic determinants on the IgG and...
Owen, F L, Ju, S T, Nisonoff, A
All A/J mice immunized with a conjugate of p-azophenylarsonate groups to keyhole limpet hemocyanin produce antibodies against azophenylarsonate, some of which share a crossreactive idiotype. The...
Laskin, J. A., Gray, A., Nisonoff, A., Klinman, N. R., Gottlieb, P. D.
Previous investigations have demonstrated close genetic linkage between loci governing expression of strain-specific idiotypes and immunoglobulin heavy (H) chain allotype (i.e., the CH locus). This...
Friedenson, B, Tung, A S, Nisonoff, A
Amino-terminal amino-acid sequences were determined for the heavy (H) chains of anti-p-azophenylarsonate antibodies with shared idiotype, isolated from nine individual A/J mice. Mice chosen for...
Role of antibody and T cells in the long-term inhibition of IgE synthesis.
We have shown that the long-term inhibition of IgE synthesis associated with perinatal inoculation of syngeneic IgE is accompanied by the synthesis of autoantibodies to IgE. Synthesis of IgE can also...
IgE-secreting cells in the thymus: correlation with induction of tolerance to IgE.
We have shown previously that normal mice become tolerant to endogenous IgE when they are approximately 2 weeks old and that this corresponds closely with the initial appearance of IgE in serum....
Lascombe, M B, Alzari, P M, Poljak, R J, Nisonoff, A
The three-dimensional structure of FabR19.9 from a well-characterized anti-p-azobenzenearsonate monoclonal antibody has been determined by x-ray diffraction techniques in two crystalline forms (I and...
Inoculation of syngeneic IgE into 2- to 12-day-old mice results in prolonged synthesis of anti-IgE antibodies without further challenge. These anti-IgE antibodies may be largely responsible for the...
Induction and properties of syngeneic murine anti-immunoglobulin D.
Haba, S, Thorbecke, G J, Nisonoff, A
High titers of autoantibodies directed to isotypic determinants of IgD were produced by inoculation of syngeneic monoclonal IgD, conjugated covalently to keyhole limpet hemocyanin, into adult or...
Wang, A. C., Wilson, S. K., Hopper, J. E., Fudenberg, H. H., Nisonoff, A.
Previous work indicated that the light chains of a monotypic immunoglobulins G2-K and M-K from a single patient (Ti1) are identical. Our present data show that the monotypic immunoglobulins G and M...
Lascombe, M B, Alzari, P M, Boulot, G, Saludjian, P, Tougard, P, Berek, C, ...
The crystal structure of Fab R19.9, derived from an anti-p-azobenzenearsonate monoclonal antibody, has been determined and refined to 2.8-A resolution by x-ray crystallographic techniques. Monoclonal...
Isotype switching by a microinjected mu immunoglobulin heavy chain gene in transgenic mice.
Durdik, J, Gerstein, R M, Rath, S, Robbins, P F, Nisonoff, A, Selsing, E
Immunization of transgenic mice carrying an immunoglobulin mu heavy chain resulted in a response dominated by expression of the transgene variable region. Unexpectedly, in a large proportion of the...
Identity of the V kappa 10-Ars-A gene segments of the A/J and BALB/c strains.
Meek, K, Sanz, I, Rathbun, G, Nisonoff, A, Capra, J D
To characterize the light chain gene segments involved in the murine immune response to keyhole limpet hemocyanin p-azophenylarsonate (Ars), we have determined the amino acid and/or nucleotide...
Induction of high titers of anti-IgE by immunization of inbred mice with syngeneic IgE.
We have generated high titers (up to 1 mg/ml) of antibodies to isotypic determinants of IgE by immunization of A/J mice with syngeneic monoclonal IgE conjugated to keyhole limpet hemocyanin. As much...
Sy, M S, Brown, A, Bach, B A, Benacerraf, B, Gottlieb, P D, Nisonoff, A, ...
Data are reported on the genetic control and structure of antigen-specific suppressive molecules obtained from azobenzenearsonate (ABA)-specific suppressor T cells (Ts). Ts-derived suppressor factors...
Influence of helper T cells on the expression of a murine intrastrain crossreactive idiotype.
Hathcock, K S, Gurish, M F, Nisonoff, A, Conger, J D, Hodes, R J
The requirement for idiotype-specific helper T (Th) cells in the generation of a major intrastrain crossreactive idiotype was investigated. This idiotype, designated CRIA, is associated with a large...
Robbins, P F, Rosen, E M, Haba, S, Nisonoff, A
We describe here an intrastrain, cross-reactive idiotype (CRI), CRID, associated with anti-p-azobenzenearsonate antibodies of the A/J strain of mouse and distinguishable, by some but not by all of...
Romano, T J, Lerman, S P, Bangasser, S, Thorbecke, G J, Nisonoff, A
Adoptive anti-trinitrophenyl (Tnp) responses were elicited from Tnp-hemocyanin (Tnp-KLH)-primed cells by challenge with an immune complex of KLH and a Tnp conjugate of the Fab fragment of rabbit...
Levin, A. S., Fudenberg, H. H., Hopper, J. E., Wilson, S. K., Nisonoff, A.
Two distinct paraproteins (IgG2-K and IgM-K) from one patient shared identical light chains and significant portions of the variable regions of the heavy chains. Idiotypic determinants on the IgG and...
Owen, F L, Ju, S T, Nisonoff, A
All A/J mice immunized with a conjugate of p-azophenylarsonate groups to keyhole limpet hemocyanin produce antibodies against azophenylarsonate, some of which share a crossreactive idiotype. The...
Laskin, J. A., Gray, A., Nisonoff, A., Klinman, N. R., Gottlieb, P. D.
Previous investigations have demonstrated close genetic linkage between loci governing expression of strain-specific idiotypes and immunoglobulin heavy (H) chain allotype (i.e., the CH locus). This...
Friedenson, B, Tung, A S, Nisonoff, A
Amino-terminal amino-acid sequences were determined for the heavy (H) chains of anti-p-azophenylarsonate antibodies with shared idiotype, isolated from nine individual A/J mice. Mice chosen for...
SEROLOGIC DEMONSTRATION OF DUAL SPECIFICITY OF RABBIT BIVALENT HYBRID ANTIBODY
Fudenberg, H. H., Drews, Genevieve, Nisonoff, A.
Hybrid, bivalent antibody molecules bearing specific combining sites for both ovalbumin and bovine gamma globulin were produced by reoxidation of a mixture of the 3.5S fragments of the two...
Sy, M-S, Dietz, MH, Nisonoff, A, Germain, RN, Benacerraf, B, Greene, MI
A/J anti-p-azobenzenearsonate (ABA) antibodies bearing cross-reactive idiotypic (CRI) determinants, when coupled to spleen cells and then injected intravenously into naive animals, stimulate...
Electron Microscopic Observations of Rabbit Antibodies
Hall, C. E., Nisonoff, A., Slayter, H. S.
Electron micrographs were obtained showing the individual, shadow-cast macromolecules from solutions of purified anti-p-azobenzoate rabbit antibody and of normal γ-globulin. The two materials look...