Inaba, M, Ando, M, Hatanaka, A, Nomoto, A, Matsuzawa, K, Tasaka, A, ...
Downregulation of natural killer cell-activating ligand CD155 by human cytomegalovirus UL141 (2005)
Tomasec, Peter, Wang, Edward Chung Yern, Davison, Andrew J., Vojtesek, Borivoj, Armstrong, Melanie, Griffin, Cora, ...
Natural killer (NK) cells are crucial in the control of cytomegalovirus infections in mice and humans. Here we show that the viral UL141 gene product has an immunomodulatory function that is...
Antigenic variation and resistance to neutralization in poliovirus type 1. (1985)
Diamond, D.C., Jameson, B.A., Bonin, J., Kohara, M., Abe, S., Itoh, H., ...
Transgenic mice susceptible to poliovirus.
Koike, S, Taya, C, Kurata, T, Abe, S, Ise, I, Yonekawa, H, ...
Poliovirus-sensitive transgenic mice were produced by introducing the human gene encoding cellular receptors for poliovirus into the mouse genome. Expression of the receptor mRNAs in tissues of the...
Functional domains of the poliovirus receptor.
A number of mutant cDNAs of the human poliovirus receptor were constructed to identify essential regions of the molecule as the receptor. All mutant cDNAs carrying the sequence coding for the entire...
Shiroki, K, Ishii, T, Aoki, T, Kobashi, M, Ohka, S, Nomoto, A
Mouse cells expressing the human poliovirus receptor (PVR-mouse cells) as well as human HeLa cells are susceptible to poliovirus type 1 Mahoney strains and produce a large amount of progeny virus at...
Host range phenotype induced by mutations in the internal ribosomal entry site of poliovirus RNA.
Shiroki, K, Ishii, T, Aoki, T, Ota, Y, Yang, W X, Komatsu, T, ...
Most poliovirus strains infect only primates. The host range (HR) of poliovirus is thought to be primarily determined by a cell surface molecule that functions as poliovirus receptor (PVR), since it...
Tsuchihara, K, Tanaka, T, Hijikata, M, Kuge, S, Toyoda, H, Nomoto, A, ...
We previously identified a highly conserved 98-nucleotide (nt) sequence, the 3'X, as the extreme 3'-terminal structure of the hepatitis C virus (HCV) genome (T. Tanaka, N. Kato, M.-J. Cho, and K....
Temperature-sensitive mouse cell factors for strand-specific initiation of poliovirus RNA synthesis.
Shiroki, K, Kato, H, Koike, S, Odaka, T, Nomoto, A
Two cell lines, TgSVA and TgSVB, were established from the kidneys of transgenic mice carrying the human gene encoding poliovirus receptor. The cells were highly susceptible to poliovirus infection,...
Koike, S, Ise, I, Sato, Y, Yonekawa, H, Gotoh, O, Nomoto, A
Using cDNA of the human poliovirus receptor (PVR) as a probe, two types of cDNA clones of the monkey homologs were isolated from a cDNA library prepared from an African green monkey kidney cell line....
Internal ribosome entry site within hepatitis C virus RNA.
Tsukiyama-Kohara, K, Iizuka, N, Kohara, M, Nomoto, A
The mechanism of initiation of translation on hepatitis C virus (HCV) RNA was investigated in vitro. HCV RNA was transcribed from the cDNA that corresponded to nucleotide positions 9 to 1772 of the...
Adenovirus E1A proteins stimulate inositol phospholipid metabolism in PC12 cells.
Shiroki, K, Yamakawa, A, Shibata, M, Takenawa, T, Sugano, S, Nomoto, A
To study the influence of nuclear oncogenes on inositol phospholipid metabolism, we examined the various parameters of inositol phospholipid metabolism in PC12 cells expressing adenovirus type 12 or...
Genetic variation occurring on the genome of an in vitro insertion mutant of poliovirus type 1.
Kuge, S, Kawamura, N, Nomoto, A
An insertion sequence of 72 nucleotides prepared from a polylinker sequence of plasmid pUC18 was introduced at nucleotide position 702 of the 5' noncoding sequence (742 nucleotides long) of the...
Kawamura, N, Kohara, M, Abe, S, Komatsu, T, Tago, K, Arita, M, ...
A number of recombinants between the virulent Mahoney and attenuated Sabin strains of type 1 poliovirus were constructed by using infectious cDNA clones of the two strains. To identify a strong...
Nucleotide sequences important for translation initiation of enterovirus RNA.
Iizuka, N, Yonekawa, H, Nomoto, A
An infectious cDNA clone was constructed from the genome of coxsackievirus B1 strain. A number of RNA transcripts that have mutations in the 5' noncoding region were synthesized in vitro from the...
Iizuka, N, Kohara, M, Hagino-Yamagishi, K, Abe, S, Komatsu, T, Tago, K, ...
Viral attenuation may be due to lowered efficiency of certain steps essential for viral multiplication. For the construction of less neurovirulent strains of poliovirus in vitro, we introduced...
In vitro construction of poliovirus defective interfering particles.
Hagino-Yamagishi, K, Nomoto, A
To construct poliovirus defective interfering (DI) particles in vitro, we synthesized an RNA from a cloned poliovirus cDNA, pSM1(T7)1, which carried a deletion in the genome region corresponding to...
Kohara, M, Abe, S, Komatsu, T, Tago, K, Arita, M, Nomoto, A
Biological tests including the monkey neurovirulence test performed on recombinants between the virulent Mahoney and attenuated Sabin 1 strains of type 1 poliovirus indicated that the genome region...
A number of deletion and insertion sequences were introduced into the 5' noncoding sequence (742 nucleotides long) of the genome of the Sabin strain of type 1 poliovirus by using an infectious cDNA...
Kohara, M, Omata, T, Kameda, A, Semler, B L, Itoh, H, Wimmer, E, ...
Infectious cDNA corresponding to the entire genome of the attenuated Sabin strain of type 1 poliovirus has been inserted into EcoRI site of bacterial plasmid pBR325. Two consecutive PstI fragments...
Analysis of RNA synthesis of type 1 poliovirus by using an in vitro molecular genetic approach.
Toyoda, H, Yang, C F, Takeda, N, Nomoto, A, Wimmer, E
Membranous crude replication complexes (CRC) were isolated from poliovirus-infected HeLa cells as recently described (N. Takeda, R.J. Kuhn, C.-F. Yang, T. Takegami, and E. Wimmer, J. Virol. 60:43-53,...
Okada, Y, Richardson, M A, Ikegami, N, Nomoto, A, Furuichi, Y
The complete nucleotide sequence of human rotavirus (Wa strain) genome segment 10 was determined by using a cloned DNA copy. The sequence data indicated that segment 10 is A + T rich (65%) and...
Richardson, M A, Iwamoto, A, Ikegami, N, Nomoto, A, Furuichi, Y
The nucleotide sequence of human (Wa) rotavirus genome segment 9, which encodes the serotype-specific antigen VP7, has been determined. Comparison of the deduced amino acid sequence of Wa VP7 protein...
Murray, M G, Kuhn, R J, Arita, M, Kawamura, N, Nomoto, A, Wimmer, E
Poliovirus exists as three stable serotypes (PV-1, PV-2, and PV-3). These viruses display three antigenic sites each, designated N-AgI, N-AgII, and N-AgIII. When mice are immunized with poliovirus,...
Chiba, H, Muramatsu, M, Nomoto, A, Kato, H
A set of genes (SWI1, SWI2/SNF2, SWI3, SNF5 and SNF6) in Saccharomyces cerevisiae are required for transcription of a variety of yeast genes. It was recently reported that the mammalian...
Hidaka, Y, Kanda, T, Iwasaki, K, Nomoto, A, Shioda, T, Shibuta, H
We determined the sequence of the 2,138 nucleotides in the Sendai virus genome just following the 3' proximal 3,686 nucleotides which we had previously reported (Nucleic Acids Res. 11, 7317-7330,...
Shioda, T, Hidaka, Y, Kanda, T, Shibuta, H, Nomoto, A, Iwasaki, K
The sequence of 3,687 nucleotides from the 3' end of the Sendai virus genome (Z strain) was determined by a molecular cloning technique followed by rapid sequence analysis. Two large open reading...
The 5' end of poliovirus mRNA is not capped with m7G(5')ppp(5')Np.
Nomoto, A, Lee, Y F, Wimmer, E
Poliovirus was grown in HeLa cells in the presence of phosphorus-32 and actinomycin D. Three to four hours after infection, viral mRNA was recovered from polyribosomes and its identity verified by...
O4-(5'-uridylyl)tyrosine is the bond between the genome-linked protein and the RNA of poliovirus.
Rothberg, P G, Harris, T J, Nomoto, A, Wimmer, E
Virion RNA of poliovirus type 1 has been analyzed for the linkage between genome-protein VPg and the polyribonucleotide chain. Hydrolysis of the linkage with acid or alkali and enzymatic degradation...
A convenient sequencing method for 5' protein-linked RNAs.
A convenient nucleotide sequencing method for 5' end protein-linked RNAs was developed. Genome of LSc, 2ab poliovirus, which has a protein (VPg) covalently linked to the 5' terminus, was labelled...
Complete nucleotide sequence of the attenuated poliovirus Sabin 1 strain genome.
Nomoto, A, Omata, T, Toyoda, H, Kuge, S, Horie, H, Kataoka, Y, ...
The complete nucleotide sequence of the genome of the type 1 poliovirus vaccine strain (LSc,2ab) was determined by using molecular cloning and rapid sequence analysis techniques. The restriction...
Yoshida, M, Yamashita, M, Nomoto, A
Transformation-defective (td) mutants with different sizes of genomic RNA were isolated from the Prague strain of Rous sarcoma virus, subgroup C(PR-C). All six td viruses (tdTYPR-C) isolated from a...
A protein covalently linked to poliovirus genome RNA.
Lee, Y F, Nomoto, A, Detjen, B M, Wimmer, E
Poliovirion [32P]RNA, after digestion with RNase T2, yields mononucleotides and a labeled compound "X," which is not negatively charged at pH 5. X contains, relative to the label in virion RNA, one...
Nomoto, A, Kitamura, N, Golini, F, Wimmer, E
The 5'-terminal, RNase T1-resistant oligonucleotide of poliovirus mRNA has been isolated. Its sequence is pU-U-A-A-A-A-C-A-Gp, which is identical to that of virion RNA except that the genome-linked...
The poliovirus receptor protein is produced both as membrane-bound and secreted forms.
Koike, S, Horie, H, Ise, I, Okitsu, A, Yoshida, M, Iizuka, N, ...
Both genomic and complementary DNA clones encoding poliovirus receptors were isolated from genomic and complementary DNA libraries prepared from HeLa S3 cells, respectively. Nucleotide sequence...
Regulation of yAP-1 nuclear localization in response to oxidative stress.
The YAP1 gene of Saccharomyces cerevisiae encodes a bZIP-containing transcription factor that is essential for the normal response of cells to oxidative stress. Under stress conditions, the activity...
Determination of functional domains in polypyrimidine-tract-binding protein.
Oh, Y L, Hahm, B, Kim, Y K, Lee, H K, Lee, J W, Song, O, ...
Polypyrimidine-tract-binding protein (PTB) is involved in pre-mRNA splicing and internal-ribosomal-entry-site-dependent translation. The biochemical properties of various segments of PTB were...
Transgenic mice susceptible to poliovirus.
Koike, S, Taya, C, Kurata, T, Abe, S, Ise, I, Yonekawa, H, ...
Poliovirus-sensitive transgenic mice were produced by introducing the human gene encoding cellular receptors for poliovirus into the mouse genome. Expression of the receptor mRNAs in tissues of the...
Functional domains of the poliovirus receptor.
A number of mutant cDNAs of the human poliovirus receptor were constructed to identify essential regions of the molecule as the receptor. All mutant cDNAs carrying the sequence coding for the entire...
Shiroki, K, Ishii, T, Aoki, T, Kobashi, M, Ohka, S, Nomoto, A
Mouse cells expressing the human poliovirus receptor (PVR-mouse cells) as well as human HeLa cells are susceptible to poliovirus type 1 Mahoney strains and produce a large amount of progeny virus at...
Host range phenotype induced by mutations in the internal ribosomal entry site of poliovirus RNA.
Shiroki, K, Ishii, T, Aoki, T, Ota, Y, Yang, W X, Komatsu, T, ...
Most poliovirus strains infect only primates. The host range (HR) of poliovirus is thought to be primarily determined by a cell surface molecule that functions as poliovirus receptor (PVR), since it...
Tsuchihara, K, Tanaka, T, Hijikata, M, Kuge, S, Toyoda, H, Nomoto, A, ...
We previously identified a highly conserved 98-nucleotide (nt) sequence, the 3'X, as the extreme 3'-terminal structure of the hepatitis C virus (HCV) genome (T. Tanaka, N. Kato, M.-J. Cho, and K....
Temperature-sensitive mouse cell factors for strand-specific initiation of poliovirus RNA synthesis.
Shiroki, K, Kato, H, Koike, S, Odaka, T, Nomoto, A
Two cell lines, TgSVA and TgSVB, were established from the kidneys of transgenic mice carrying the human gene encoding poliovirus receptor. The cells were highly susceptible to poliovirus infection,...
Koike, S, Ise, I, Sato, Y, Yonekawa, H, Gotoh, O, Nomoto, A
Using cDNA of the human poliovirus receptor (PVR) as a probe, two types of cDNA clones of the monkey homologs were isolated from a cDNA library prepared from an African green monkey kidney cell line....
Internal ribosome entry site within hepatitis C virus RNA.
Tsukiyama-Kohara, K, Iizuka, N, Kohara, M, Nomoto, A
The mechanism of initiation of translation on hepatitis C virus (HCV) RNA was investigated in vitro. HCV RNA was transcribed from the cDNA that corresponded to nucleotide positions 9 to 1772 of the...
Adenovirus E1A proteins stimulate inositol phospholipid metabolism in PC12 cells.
Shiroki, K, Yamakawa, A, Shibata, M, Takenawa, T, Sugano, S, Nomoto, A
To study the influence of nuclear oncogenes on inositol phospholipid metabolism, we examined the various parameters of inositol phospholipid metabolism in PC12 cells expressing adenovirus type 12 or...
Genetic variation occurring on the genome of an in vitro insertion mutant of poliovirus type 1.
Kuge, S, Kawamura, N, Nomoto, A
An insertion sequence of 72 nucleotides prepared from a polylinker sequence of plasmid pUC18 was introduced at nucleotide position 702 of the 5' noncoding sequence (742 nucleotides long) of the...
Kawamura, N, Kohara, M, Abe, S, Komatsu, T, Tago, K, Arita, M, ...
A number of recombinants between the virulent Mahoney and attenuated Sabin strains of type 1 poliovirus were constructed by using infectious cDNA clones of the two strains. To identify a strong...
Nucleotide sequences important for translation initiation of enterovirus RNA.
Iizuka, N, Yonekawa, H, Nomoto, A
An infectious cDNA clone was constructed from the genome of coxsackievirus B1 strain. A number of RNA transcripts that have mutations in the 5' noncoding region were synthesized in vitro from the...
Iizuka, N, Kohara, M, Hagino-Yamagishi, K, Abe, S, Komatsu, T, Tago, K, ...
Viral attenuation may be due to lowered efficiency of certain steps essential for viral multiplication. For the construction of less neurovirulent strains of poliovirus in vitro, we introduced...
In vitro construction of poliovirus defective interfering particles.
Hagino-Yamagishi, K, Nomoto, A
To construct poliovirus defective interfering (DI) particles in vitro, we synthesized an RNA from a cloned poliovirus cDNA, pSM1(T7)1, which carried a deletion in the genome region corresponding to...
Kohara, M, Abe, S, Komatsu, T, Tago, K, Arita, M, Nomoto, A
Biological tests including the monkey neurovirulence test performed on recombinants between the virulent Mahoney and attenuated Sabin 1 strains of type 1 poliovirus indicated that the genome region...
A number of deletion and insertion sequences were introduced into the 5' noncoding sequence (742 nucleotides long) of the genome of the Sabin strain of type 1 poliovirus by using an infectious cDNA...
Kohara, M, Omata, T, Kameda, A, Semler, B L, Itoh, H, Wimmer, E, ...
Infectious cDNA corresponding to the entire genome of the attenuated Sabin strain of type 1 poliovirus has been inserted into EcoRI site of bacterial plasmid pBR325. Two consecutive PstI fragments...
Analysis of RNA synthesis of type 1 poliovirus by using an in vitro molecular genetic approach.
Toyoda, H, Yang, C F, Takeda, N, Nomoto, A, Wimmer, E
Membranous crude replication complexes (CRC) were isolated from poliovirus-infected HeLa cells as recently described (N. Takeda, R.J. Kuhn, C.-F. Yang, T. Takegami, and E. Wimmer, J. Virol. 60:43-53,...
Okada, Y, Richardson, M A, Ikegami, N, Nomoto, A, Furuichi, Y
The complete nucleotide sequence of human rotavirus (Wa strain) genome segment 10 was determined by using a cloned DNA copy. The sequence data indicated that segment 10 is A + T rich (65%) and...
Richardson, M A, Iwamoto, A, Ikegami, N, Nomoto, A, Furuichi, Y
The nucleotide sequence of human (Wa) rotavirus genome segment 9, which encodes the serotype-specific antigen VP7, has been determined. Comparison of the deduced amino acid sequence of Wa VP7 protein...
Murray, M G, Kuhn, R J, Arita, M, Kawamura, N, Nomoto, A, Wimmer, E
Poliovirus exists as three stable serotypes (PV-1, PV-2, and PV-3). These viruses display three antigenic sites each, designated N-AgI, N-AgII, and N-AgIII. When mice are immunized with poliovirus,...
Chiba, H, Muramatsu, M, Nomoto, A, Kato, H
A set of genes (SWI1, SWI2/SNF2, SWI3, SNF5 and SNF6) in Saccharomyces cerevisiae are required for transcription of a variety of yeast genes. It was recently reported that the mammalian...
Hidaka, Y, Kanda, T, Iwasaki, K, Nomoto, A, Shioda, T, Shibuta, H
We determined the sequence of the 2,138 nucleotides in the Sendai virus genome just following the 3' proximal 3,686 nucleotides which we had previously reported (Nucleic Acids Res. 11, 7317-7330,...
Shioda, T, Hidaka, Y, Kanda, T, Shibuta, H, Nomoto, A, Iwasaki, K
The sequence of 3,687 nucleotides from the 3' end of the Sendai virus genome (Z strain) was determined by a molecular cloning technique followed by rapid sequence analysis. Two large open reading...
The 5' end of poliovirus mRNA is not capped with m7G(5')ppp(5')Np.
Nomoto, A, Lee, Y F, Wimmer, E
Poliovirus was grown in HeLa cells in the presence of phosphorus-32 and actinomycin D. Three to four hours after infection, viral mRNA was recovered from polyribosomes and its identity verified by...
O4-(5'-uridylyl)tyrosine is the bond between the genome-linked protein and the RNA of poliovirus.
Rothberg, P G, Harris, T J, Nomoto, A, Wimmer, E
Virion RNA of poliovirus type 1 has been analyzed for the linkage between genome-protein VPg and the polyribonucleotide chain. Hydrolysis of the linkage with acid or alkali and enzymatic degradation...
A convenient sequencing method for 5' protein-linked RNAs.
A convenient nucleotide sequencing method for 5' end protein-linked RNAs was developed. Genome of LSc, 2ab poliovirus, which has a protein (VPg) covalently linked to the 5' terminus, was labelled...
Complete nucleotide sequence of the attenuated poliovirus Sabin 1 strain genome.
Nomoto, A, Omata, T, Toyoda, H, Kuge, S, Horie, H, Kataoka, Y, ...
The complete nucleotide sequence of the genome of the type 1 poliovirus vaccine strain (LSc,2ab) was determined by using molecular cloning and rapid sequence analysis techniques. The restriction...
Yoshida, M, Yamashita, M, Nomoto, A
Transformation-defective (td) mutants with different sizes of genomic RNA were isolated from the Prague strain of Rous sarcoma virus, subgroup C(PR-C). All six td viruses (tdTYPR-C) isolated from a...
A protein covalently linked to poliovirus genome RNA.
Lee, Y F, Nomoto, A, Detjen, B M, Wimmer, E
Poliovirion [32P]RNA, after digestion with RNase T2, yields mononucleotides and a labeled compound "X," which is not negatively charged at pH 5. X contains, relative to the label in virion RNA, one...
Nomoto, A, Kitamura, N, Golini, F, Wimmer, E
The 5'-terminal, RNase T1-resistant oligonucleotide of poliovirus mRNA has been isolated. Its sequence is pU-U-A-A-A-A-C-A-Gp, which is identical to that of virion RNA except that the genome-linked...
The poliovirus receptor protein is produced both as membrane-bound and secreted forms.
Koike, S, Horie, H, Ise, I, Okitsu, A, Yoshida, M, Iizuka, N, ...
Both genomic and complementary DNA clones encoding poliovirus receptors were isolated from genomic and complementary DNA libraries prepared from HeLa S3 cells, respectively. Nucleotide sequence...
Regulation of yAP-1 nuclear localization in response to oxidative stress.
The YAP1 gene of Saccharomyces cerevisiae encodes a bZIP-containing transcription factor that is essential for the normal response of cells to oxidative stress. Under stress conditions, the activity...
Determination of functional domains in polypyrimidine-tract-binding protein.
Oh, Y L, Hahm, B, Kim, Y K, Lee, H K, Lee, J W, Song, O, ...
Polypyrimidine-tract-binding protein (PTB) is involved in pre-mRNA splicing and internal-ribosomal-entry-site-dependent translation. The biochemical properties of various segments of PTB were...