Hayashi, K, Nakazawa, M, Ishizaki, Y, Hiraoka, N, Obayashi, A
Polyethylene glycol (PEG) stimulates ligation with T4 DNA ligase. In 10% (w/v) PEG 6,000 solutions, only intermolecular ligation is enhanced by monovalent cations, while both inter- and...
AccIII, a new restriction endonuclease from Acinetobacter calcoaceticus.
Kita, K, Hiraoka, N, Oshima, A, Kadonishi, S, Obayashi, A
A new site-specific restriction endonuclease, AccIII, was isolated from Acinetobacter calcoaceticus. AccIII recognizes T/CCGGA and cleaves at the position shown by the arrow. AccIII activity was...
Interaction of the restriction endonuclease ScaI with its substrates.
Kita, K, Hiraoka, N, Kimizuka, F, Obayashi, A, Kojima, H, Takahashi, H, ...
The kinetic constants of the site-specific endonuclease, ScaI, for various substrates were determined. We estimated Vmax and Km for octa-, deca-, dodeca-, and hexadecanucleotides and for plasmid...
Hayashi, K, Nakazawa, M, Ishizaki, Y, Hiraoka, N, Obayashi, A
In the presence of high concentrations of the nonspecific polymer polyethylene glycol (PEG), intermolecular cohesive-end ligation with the DNA ligase from Escherichia coli was stimulated by high salt...
A new restriction endonuclease from Spirulina platensis.
Kawamura, M, Sakakibara, M, Watanabe, T, Kita, K, Hiraoka, N, Obayashi, A, ...
Three restriction endonucleases, Sp1I, Sp1II and Sp1III have been purified partially from Spirulina platensis subspecies siamese and named. Sp1I cleaves bacteriophage lambda DNA at one site, phi X...
Nucleotide sequence and expression of the cloned gene of bacteriophage SP6 RNA polymerase.
Kotani, H, Ishizaki, Y, Hiraoka, N, Obayashi, A
The coding region of the gene for bacteriophage SP6 RNA polymerase was cloned into pBR322, and its entire nucleotide sequence was deduced. The predicted amino acid sequence for the polymerase...
Hayashi, K, Nakazawa, M, Ishizaki, Y, Obayashi, A
Monovalent cations such as Na+ and K+ inhibit the activity of T4 DNA ligase. However, the extent of inhibition varies with the terminal sequence of the duplex DNA used as substrate; in many cases,...
Hayashi, K, Nakazawa, M, Ishizaki, Y, Hiraoka, N, Obayashi, A
Polyethylene glycol (PEG) stimulates ligation with T4 DNA ligase. In 10% (w/v) PEG 6,000 solutions, only intermolecular ligation is enhanced by monovalent cations, while both inter- and...
AccIII, a new restriction endonuclease from Acinetobacter calcoaceticus.
Kita, K, Hiraoka, N, Oshima, A, Kadonishi, S, Obayashi, A
A new site-specific restriction endonuclease, AccIII, was isolated from Acinetobacter calcoaceticus. AccIII recognizes T/CCGGA and cleaves at the position shown by the arrow. AccIII activity was...
Interaction of the restriction endonuclease ScaI with its substrates.
Kita, K, Hiraoka, N, Kimizuka, F, Obayashi, A, Kojima, H, Takahashi, H, ...
The kinetic constants of the site-specific endonuclease, ScaI, for various substrates were determined. We estimated Vmax and Km for octa-, deca-, dodeca-, and hexadecanucleotides and for plasmid...
Hayashi, K, Nakazawa, M, Ishizaki, Y, Hiraoka, N, Obayashi, A
In the presence of high concentrations of the nonspecific polymer polyethylene glycol (PEG), intermolecular cohesive-end ligation with the DNA ligase from Escherichia coli was stimulated by high salt...
A new restriction endonuclease from Spirulina platensis.
Kawamura, M, Sakakibara, M, Watanabe, T, Kita, K, Hiraoka, N, Obayashi, A, ...
Three restriction endonucleases, Sp1I, Sp1II and Sp1III have been purified partially from Spirulina platensis subspecies siamese and named. Sp1I cleaves bacteriophage lambda DNA at one site, phi X...
Nucleotide sequence and expression of the cloned gene of bacteriophage SP6 RNA polymerase.
Kotani, H, Ishizaki, Y, Hiraoka, N, Obayashi, A
The coding region of the gene for bacteriophage SP6 RNA polymerase was cloned into pBR322, and its entire nucleotide sequence was deduced. The predicted amino acid sequence for the polymerase...
Hayashi, K, Nakazawa, M, Ishizaki, Y, Obayashi, A
Monovalent cations such as Na+ and K+ inhibit the activity of T4 DNA ligase. However, the extent of inhibition varies with the terminal sequence of the duplex DNA used as substrate; in many cases,...