A. Ohta

Publication List Details

Period

1990 - 2008

Number

57

Co-Authors

Effects of ultrasound on the proliferation and differentiation of cementoblast lineage cells (2008)

Inubushi, T., Tanaka, E., Rego, E.B., Kitagawa, M., Kawazoe, A., Ohta, A., ...

Background: The purpose of this study was to investigate the effects of low-intensity pulsed ultrasound (LIPUS) stimulation on the proliferation and differentiation of cementoblast lineage cells....

Cutting edge: hypoxia-inducible factor 1alpha and its activation-inducible short isoform I.1 negatively regulate functions of CD4+ and CD8+ T lymphocytes. (2006)

Lukashev, D, Klebanov, B, Kojima, H, Grinberg, A, Ohta, A, Berenfeld, L, ...

To evaluate the role of hypoxia-inducible factor 1alpha (HIF-1alpha) and its TCR activation-inducible short isoform I.1 in T cell functions, we genetically engineered unique mice with: 1) knockout of...

Ex vivo CD4 + T-cell cytokine expression from patients with Sjogren's syndrome following in vitro stimulation to induce proliferation (2006)

Koarada, S., Haruta, Y., Mitamura, M., Morito, F., Tada, Y., Ohta, A., ...

Objective. To assess ex vivo CD4+ T-cell cytokine expression from patients with primary Sjögren's syndrome (SS) following in vitro stimulation to induce proliferation, as proliferation is...

Ex vivo CD4+ T-cell cytokine expression from patients with Sjogren's syndrome following in vitro stimulation to induce proliferation (2005)

Koarada, S., Haruta, Y., Mitamura, M., Morito, F., Tada, Y., Ohta, A., ...

Objective. To assess ex vivo CD4+ T-cell cytokine expression from patients with primary Sjögren's syndrome (SS) following in vitro stimulation to induce proliferation, as proliferation is closely...

Increased entry of CD4+ T cells into the Th1 cytokine effector pathway during T-cell division following stimulation in Behcet's disease (2004)

Koarada, S., Haruta, Y., Tada, Y., Ushiyama, O., Morito, F., Ohta, A., ...

Objectives. To investigate the relationship between the production of Th1/Th2 cytokines and cell kinetics, cell division and proliferation in patients with Behçet's disease (BD). Methods. Peripheral...

Increased entry of CD4+ T cells into the Th1 cytokine effector pathway during T-cell division following stimulation in Behcet's disease (2004)

Koarada, S., Haruta, Y., Tada, Y., Ushiyama, O., Morito, F., Ohta, A., ...

Objectives. To investigate the relationship between the production of Th1/Th2 cytokines and cell kinetics, cell division and proliferation in patients with Behçet's disease (BD). Methods....

Increased entry of CD4+ T cells into the Th1 cytokine effector pathway during T-cell division following stimulation in Behcet's disease (2004)

Koarada, S., Haruta, Y., Tada, Y., Ushiyama, O., Morito, F., Ohta, A., ...

Objectives. To investigate the relationship between the production of Th1/Th2 cytokines and cell kinetics, cell division and proliferation in patients with Behçet's disease (BD). Methods. Peripheral...

Inducible expression of a gene encoding an L41 ribosomal protein responsible for the cycloheximide-resistant phenotype in the yeast Candida maltosa.

Mutoh, E, Mochizuki, M, Ohta, A, Takagi, M

In a previous paper (S. Kawai, S. Murao, M. Mochizuki, I. Shibuya, K. Yano, and M. Takagi, J. Bacteriol. 174:254-262, 1992), we showed that in each genome of several yeast species, there is one of...

Evidence that part of a centromeric DNA region induces pseudohyphal growth in a dimorphic yeast, Candida maltosa.

Nakazawa, T, Motoyama, T, Horiuchi, H, Ohta, A, Takagi, M

We observed that a YCp-type vector having the centromeric DNA (CEN) sequence previously isolated from the genome, but not a YRp-type vector lacking the CEN sequence, induced pseudohyphal growth in a...

Purification of two chitinases from Rhizopus oligosporus and isolation and sequencing of the encoding genes.

Yanai, K, Takaya, N, Kojima, N, Horiuchi, H, Ohta, A, Takagi, M

Two chitinases were purified from Rhizopus oligosporus, a filamentous fungus belonging to the class Zygomycetes, and designated chitinase I and chitinase II. Their N-terminal amino acid sequences...

Suppression of the lethal effect of acidic-phospholipid deficiency by defective formation of the major outer membrane lipoprotein in Escherichia coli.

Asai, Y, Katayose, Y, Hikita, C, Ohta, A, Shibuya, I

The Escherichia coli pgsA3 allele encoding a defective phosphatidylglycerophosphate synthase is lethal for all but certain strains. Genetic analysis of such strains has revealed that the lethal...

Disruption of the Escherichia coli cls gene responsible for cardiolipin synthesis.

Nishijima, S, Asami, Y, Uetake, N, Yamagoe, S, Ohta, A, Shibuya, I

The cls gene of Escherichia coli is responsible for the synthesis of a major membrane phospholipid, cardiolipin, and has been proposed to encode cardiolipin synthase. This gene cloned on a pBR322...

Biosynthesis of novel acidic phospholipid analogs in Escherichia coli.

Shibuya, I, Yamagoe, S, Miyazaki, C, Matsuzaki, H, Ohta, A

When cultured in the presence of 600 mM D-mannitol, Escherichia coli K-12 cells synthesized two novel phospholipids. The identities of these compounds are postulated to be phosphatidylmannitol and...

Alteration of phospholipid composition by combined defects in phosphatidylserine and cardiolipin synthases and physiological consequences in Escherichia coli.

Shibuya, I, Miyazaki, C, Ohta, A

Escherichia coli K-12 derivatives with a common genetic background carrying, either alone or in combination, the pss-1 allele coding for a temperature-sensitive phosphatidylserine synthase (A. Ohta...

Cloning of genes involved in membrane lipid synthesis: effects of amplification of phosphatidylglycerophosphate synthase in Escherichia coli.

Ohta, A, Waggoner, K, Radominska-Pyrek, A, Dowhan, W

The structural gene (pgsA) for the CDP-diacylglycerol:sn-glycero-3-phosphate phosphatidyltransferase (EC 2.7.8.5, phosphatidylglycerophosphate synthase) from Escherichia coli has been cloned, using...

Molecular cloning of the cls gene responsible for cardiolipin synthesis in Escherichia coli and phenotypic consequences of its amplification.

Ohta, A, Obara, T, Asami, Y, Shibuya, I

The cls gene responsible for cardiolipin synthesis in Escherichia coli K-12 was cloned in a 5-kilobase-pair DNA fragment inserted in a mini-F vector, pML31, and then subcloned into a...

Membrane phospholipid synthesis and phenotypic correlation of an Escherichia coli pss mutant.

Ohta, A, Shibuya, I

A pair of putatively isogenic pss(Ts) and pss+ (phosphatidylserine synthetase structural gene) strains was constructed and analyzed, together with the revertants, for the physiological consequences...

In vitro and in vivo antifungal activities of BMY-28864, a water-soluble pradimicin derivative.

Kakushima, M, Masuyoshi, S, Hirano, M, Shinoda, M, Ohta, A, Kamei, H, ...

BMY-28864, a water-soluble pradimicin derivative, had potent in vitro activity against a wide variety of fungi, including those associated with deep-seated mycosis; it inhibited the growth of...

Cloning of the uvrC gene of Escherichia coli: expression of a DNA repair gene.

Sharma, S, Ohta, A, Dowhan, W, Moses, R E

We have cloned the uvrC gene of Escherichia coli, using an F' plasmid carrying the uvrC region as a source of DNA. Two plasmids, pSC101 and pBR322, were used as cloning vectors. The recombinant...

Genetic manipulation of membrane phospholipid composition in Escherichia coli: pgsA mutants defective in phosphatidylglycerol synthesis.

Miyazaki, C, Kuroda, M, Ohta, A, Shibuya, I

Unique mutants of Escherichia coli K-12, defective in phosphatidylglycerol synthesis, have been isolated from a temperature-sensitive strain incubated at its nonpermissive temperature. The parent...

Identification of the 32 kDa components of bovine lens EDTA-extractable protein as endonexins I and II.

Kobayashi, R, Nakayama, R, Ohta, A, Sakai, F, Sakuragi, S, Tashima, Y

EDTA-extractable protein (EEP) is a mixture of major lens membrane proteins with molecular masses ranging from 32 kDa to 40 kDa. These bind to the lens membrane in a Ca2(+)-dependent manner. In the...

Observing responses maintained by conditional discriminative stimuli.

Ohta, A

In a conditional discrimination procedure, pigeons' observing responses were analyzed to examine whether two color stimuli (blue or red), conditionally related to whether each of two line stimuli...

Conditioned reinforcement by conditional discriminative stimuli.

Ohta, A

A concurrent-chains schedule was used to examine how a delay to conditional discriminative stimuli affects conditioned reinforcement strength. Pigeons' key-peck responses in the initial link produced...

Inducible expression of a gene encoding an L41 ribosomal protein responsible for the cycloheximide-resistant phenotype in the yeast Candida maltosa.

Mutoh, E, Mochizuki, M, Ohta, A, Takagi, M

In a previous paper (S. Kawai, S. Murao, M. Mochizuki, I. Shibuya, K. Yano, and M. Takagi, J. Bacteriol. 174:254-262, 1992), we showed that in each genome of several yeast species, there is one of...

Evidence that part of a centromeric DNA region induces pseudohyphal growth in a dimorphic yeast, Candida maltosa.

Nakazawa, T, Motoyama, T, Horiuchi, H, Ohta, A, Takagi, M

We observed that a YCp-type vector having the centromeric DNA (CEN) sequence previously isolated from the genome, but not a YRp-type vector lacking the CEN sequence, induced pseudohyphal growth in a...

Purification of two chitinases from Rhizopus oligosporus and isolation and sequencing of the encoding genes.

Yanai, K, Takaya, N, Kojima, N, Horiuchi, H, Ohta, A, Takagi, M

Two chitinases were purified from Rhizopus oligosporus, a filamentous fungus belonging to the class Zygomycetes, and designated chitinase I and chitinase II. Their N-terminal amino acid sequences...

Suppression of the lethal effect of acidic-phospholipid deficiency by defective formation of the major outer membrane lipoprotein in Escherichia coli.

Asai, Y, Katayose, Y, Hikita, C, Ohta, A, Shibuya, I

The Escherichia coli pgsA3 allele encoding a defective phosphatidylglycerophosphate synthase is lethal for all but certain strains. Genetic analysis of such strains has revealed that the lethal...

Disruption of the Escherichia coli cls gene responsible for cardiolipin synthesis.

Nishijima, S, Asami, Y, Uetake, N, Yamagoe, S, Ohta, A, Shibuya, I

The cls gene of Escherichia coli is responsible for the synthesis of a major membrane phospholipid, cardiolipin, and has been proposed to encode cardiolipin synthase. This gene cloned on a pBR322...

Biosynthesis of novel acidic phospholipid analogs in Escherichia coli.

Shibuya, I, Yamagoe, S, Miyazaki, C, Matsuzaki, H, Ohta, A

When cultured in the presence of 600 mM D-mannitol, Escherichia coli K-12 cells synthesized two novel phospholipids. The identities of these compounds are postulated to be phosphatidylmannitol and...

Alteration of phospholipid composition by combined defects in phosphatidylserine and cardiolipin synthases and physiological consequences in Escherichia coli.

Shibuya, I, Miyazaki, C, Ohta, A

Escherichia coli K-12 derivatives with a common genetic background carrying, either alone or in combination, the pss-1 allele coding for a temperature-sensitive phosphatidylserine synthase (A. Ohta...

Cloning of genes involved in membrane lipid synthesis: effects of amplification of phosphatidylglycerophosphate synthase in Escherichia coli.

Ohta, A, Waggoner, K, Radominska-Pyrek, A, Dowhan, W

The structural gene (pgsA) for the CDP-diacylglycerol:sn-glycero-3-phosphate phosphatidyltransferase (EC 2.7.8.5, phosphatidylglycerophosphate synthase) from Escherichia coli has been cloned, using...

Molecular cloning of the cls gene responsible for cardiolipin synthesis in Escherichia coli and phenotypic consequences of its amplification.

Ohta, A, Obara, T, Asami, Y, Shibuya, I

The cls gene responsible for cardiolipin synthesis in Escherichia coli K-12 was cloned in a 5-kilobase-pair DNA fragment inserted in a mini-F vector, pML31, and then subcloned into a...

Membrane phospholipid synthesis and phenotypic correlation of an Escherichia coli pss mutant.

Ohta, A, Shibuya, I

A pair of putatively isogenic pss(Ts) and pss+ (phosphatidylserine synthetase structural gene) strains was constructed and analyzed, together with the revertants, for the physiological consequences...

In vitro and in vivo antifungal activities of BMY-28864, a water-soluble pradimicin derivative.

Kakushima, M, Masuyoshi, S, Hirano, M, Shinoda, M, Ohta, A, Kamei, H, ...

BMY-28864, a water-soluble pradimicin derivative, had potent in vitro activity against a wide variety of fungi, including those associated with deep-seated mycosis; it inhibited the growth of...

Cloning of the uvrC gene of Escherichia coli: expression of a DNA repair gene.

Sharma, S, Ohta, A, Dowhan, W, Moses, R E

We have cloned the uvrC gene of Escherichia coli, using an F' plasmid carrying the uvrC region as a source of DNA. Two plasmids, pSC101 and pBR322, were used as cloning vectors. The recombinant...

Genetic manipulation of membrane phospholipid composition in Escherichia coli: pgsA mutants defective in phosphatidylglycerol synthesis.

Miyazaki, C, Kuroda, M, Ohta, A, Shibuya, I

Unique mutants of Escherichia coli K-12, defective in phosphatidylglycerol synthesis, have been isolated from a temperature-sensitive strain incubated at its nonpermissive temperature. The parent...

Identification of the 32 kDa components of bovine lens EDTA-extractable protein as endonexins I and II.

Kobayashi, R, Nakayama, R, Ohta, A, Sakai, F, Sakuragi, S, Tashima, Y

EDTA-extractable protein (EEP) is a mixture of major lens membrane proteins with molecular masses ranging from 32 kDa to 40 kDa. These bind to the lens membrane in a Ca2(+)-dependent manner. In the...

Observing responses maintained by conditional discriminative stimuli.

Ohta, A

In a conditional discrimination procedure, pigeons' observing responses were analyzed to examine whether two color stimuli (blue or red), conditionally related to whether each of two line stimuli...

Conditioned reinforcement by conditional discriminative stimuli.

Ohta, A

A concurrent-chains schedule was used to examine how a delay to conditional discriminative stimuli affects conditioned reinforcement strength. Pigeons' key-peck responses in the initial link produced...

Collagenase production by human mononuclear cells in culture: inhibition by gold containing compounds and other antirheumatic agents.

Ohta, A, Louie, J S, Uitto, J

Human peripheral blood mononuclear cells in adherent cultures have been shown to synthesise and secrete collagenase. In the present study we have examined the modulation of collagenase production in...

Retinoid modulation of collagenase production by adherent human mononuclear cells in culture.

Ohta, A, Louie, J S, Uitto, J

Previous observations have suggested that retinoids might be useful for the treatment of rheumatoid arthritis. In this study we examined the effects of various retinoids on collagenase production by...

Retinal disease in patients with systemic lupus erythematosus

Ushiyama, O., Ushiyama, K., Koarada, S., Tada, Y., Suzuki, N., Ohta, A., ...

OBJECTIVE—To investigate the incidence of retinopathy in systemic lupus erythematosus (SLE) and to clarify its significance in relation to other clinical manifestations.
METHODS—A cross...

Difference in B cell activation between dermatomyositis and polymyositis: analysis of the expression of RP105 on peripheral blood B cells

Kikuchi, Y, Koarada, S, Tada, Y, Ushiyama, O, Morito, F, Suzuki, N, ...

BACKGROUND—It has previously been shown that RP105, a new B cell surface protein, is lost in activated human B cells.
OBJECTIVE—To investigate whether there is a difference in B cell activation...

Retinal findings in systemic sclerosis: a comparison with nailfold capillaroscopic patterns

Ushiyama, O, Ushiyama, K, Yamada, T, Koarada, S, Tada, Y, Suzuki, N, ...

Objective: To determine the prevalence of retinal disease in systemic sclerosis (SSc) and to characterise the findings of retinopathy. Additionally, to analyse the association of retinal disease with...

Adenosine A2A receptor antagonists: blockade of adenosinergic effects and T regulatory cells

Sitkovsky, M, Lukashev, D, Deaglio, S, Dwyer, K, Robson, S C, Ohta, A

The intensity and duration of host responses are determined by protective mechanisms that control tissue injury by dampening down inflammation. Adenosine generation and consequent effects, mediated...